GOST ISO 15163-2014 Milk and milk products Calf rennet and adult bovine rennet Determination of chymosin and bovine pepsin contents by chromatography《乳和乳制品 牛犊皱胃酶和成年牛皱胃酶 采用色谱法测定凝乳酶和.pdf

1、 (ISO 15163:2012, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 151632014, - 1.092 . - 1.22009 . , . -, , , 1 - - , 52 ()3 , (- 30 2014 . 67-) : ( 3166) 00497 ( 3166) 00497 AMBYKGMDRUTJ -4 9 2014 . 718- ISO 151632014 1 2016 .5 ISO 15163:2012 Milk and milkproduc

2、ts Calf rennet and adult bovine rennet Determination by chromatography of chymosin and bovinepepsin contents ( . . ). SC 5 - ISO/TC 34 (ISO) (IDF). (en). , , . - . (IDT)6 II ISO 151632014 -, - . () . , - - , 2015 -, III ISO 1516320141 .12 13 .14 25 .36 .37 47.1 .47.2 Fractogel .47.3 Fractogel .47.4

3、Fractogel .47.5 .47.6 .57.7 78 .78.1 , 78.2 , 378.3 89 89.1 89.2 89.3 .910 9() - .10B() .14() 1516IV ISO 151632014 () : -. ( ) . - . , 5, 6. , - . , , pH, , , , . ( ) 6, 7.V ISO 151632014 Milk and milk products. Calf rennet and adult bovine rennet.Determination of chymosin and bovine pepsin contents

4、 by chromatography1 - . , , .2 - . . ( ).ISO 11815|IDF 157:2007 Milk Determination of total milk-clotting activity of bovine rennets (. )3 , - 8, 9. ISO 11815|IDF 157 ( 6,5). - - (), 1 3 - 1 3 . 1000 /. - (. ISO 11815|IDF 157). , . , , . - (. 10).1 ISO 151632014 201601014 , - , .4.1 , FractogelEMD D

5、EAE (M) (Merck cat. no. 1.16883)1) Mono Q13 (HR 5/5 5/50 GL GE Healthcare)2) .1 FractogelEMD DEAE (M) , Mono Q - .2 Fractogel Mono Q , 4.12, .4.2 (C4H10N2c215 6H2O).4.3 (NaCl).4.4 , .4.5 (NaOH).4.6 , c(HCl) = 1 /3.4.7 (C2H5OH), 96 %.4.8 (C2H5OH), 20 %. 105 396 %- (4.7) 400 3 . - , .4.9 , c(N2H4CO) =

6、 8 /3 48 100 3.4.10 , 1 (Union Carbide)3) (). .4.11 , Bio-Rad-Econopac 10DG (cat. no. 732-2010)4) (). (4.10), .4.12 4.12.1 I, (CH2)4(NH)2, c(CH2)4(NH)2 = 0,025 /3 4,85 (4.2) 42,8 (4.6) . - 1000 3(5.5), . - (5,30 c177 0,05) . . . 4.12.5.4.12.2 II, c(NaCl) = 0,25 /3 1000 3(5.5) 14,6 NaCl. I (4.12.1) .

7、 (pH) . II . - 4.12.5.1)FractogelEMD DEAE (M) . - ISO IDF.2)Mono Q13 . - ISO IDF.3)Union Carbide . ISO IDF.4)Bio-Rad Econopac 10DG . - ISO IDF.2 ISO 1516320144.12.3 III, c(NaCl) = 0,50 /3 1000 3(5.5) 29,2 NaCl. I (4.12.1) . (pH) . III . 4.12.5.4.12.4 IV, c(NaCl) = 1,0 /3 1000 3(5.5) 58,4 NaCl. I (4.12.1) . (pH) . III . 4.12.5.4.12.5 I IV (4.12.14.12.4) . I IV , , , 0,2 . I IV .5 5.1 ( ).5.2 pH-, () 0,01 . .5.3 , 1,0 10 , , 5 ( ).5.4 .5.5 , 2.5.6 FPLC1), c63KTA2) HPLC , , .5.7 ,(. ISO11815|IDF157).6 , . - 1. 1, 9, () 13. . . . 0 C 5 C.1)FPLC . ISO IDF.2)c63KTA .