1、 . (ISO 15174:2012, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 151742014, - 1.092 . 1.22009 . -, . , , 1 - , 52 (-)3 , ( 30 2014 . 67-) : ( 3166) 00497 ( 3166) 00497 AMBYKGMDRUTJ -4 9 2014 . 707- ISO 151742014 1 2016 .5 ISO 15174:2012 Milk and milkproducts M
2、icrobial coagulants Determination of total milk-clotting activity ( -. . ). SC 5 - ISO/TC 34 (ISO) (IDF). (en). , , . (IDT)6 II ISO 151742014 -, - . () . , - - , 2015 -, III ISO 151742014 .V1 .12 .13 .14 .35 .36 36.1 36.2 .37 47.1 .47.2 47.3 47.4 .58 .58.1 .58.2 69 69.1 69.2 69.3 .610 7 A () 8 9IV I
3、SO 151742014 , - Rhizomucor miehei ( 3.4.23.23), R. pusillus ( 3.4.23.23) Cryphonectriaparasitica, Endothia parasitica ( 3.4.23.22). , - , , . - , , , . , , - . , R. miehei, . , ISO 11815|IDF 157. 7, A. .V ISO 151742014 . Milk and milk products.Microbial coagulants. Determination of total milk-clott
4、ing activity1 , 0,5 /3(pH = 6,50 .).2 , , 0,5 /3(pH = 6,50 .) - .3 , - , .3.1 , pH 5,5. 1 3 10,0 (CH3COONa c215 3H2O), (4.1) 10,0 3 1 /3(CH3COOH) . -. pH 5,5 . 1 /3 1 /3.3.2 , (CaCl2) = 500 /3. 500 /3 , 1). . (18 C22 C). EDTA( ) .1)Chr. Hansens A/S, Hvidovre, Denmark . ISO IDF.1 ISO 151742014 201601
5、013.3 , c114 (CaCl2)=0,5/3. (3.2) , 0,5 /3. 2,00 3 c114(CaCl2) = 500 /3, 2,70 . (3.2), . 2000 3 2,70 - (3.2) 0,01 , 18 22 . . . 50 /3 .3.4 - , . 1).3.5 (Rhizomucor miehei), - . . - 18 C . , , , . - ; , . (R. miehei) , , , , 1000 IMCU/ (. 6). - . / . , . () , , NIZO2). DSM Food Specialties3).1)Cecala
6、it, Poligny, France . ISO IDF.2)NIZO Food Research BV, Ede, Netherlands . - ISO IDF.3)DSMFoodSpecialties,DairyIngredientsGroup,Delft,Netherlands-. ISO IDF.2 ISO 1517420144 , , .4.1 , 0,5 3/ 0,2 % .4.2 , 1, A. (, ) . 0,4 %.4.3 , 3, A.4.4 , , 20 c176C45c176C, c1770,1 C.4.5 pH-, () - 0,01 . pH.4.6 , c1771.4.7 , 1 .4.8 (. 7.4).4.9 , (32 c177 1) , c1770,2 . -.4.9.1 , - (4.8), 30 . . - 2 4 /.4.9.2 , (4.8). , .5 , . (6.1) 2 ( 9) - (6.2) 2 ( 13). , . 0 C 5 C.6 6.1 , . (7.3) - (18 C22 C). . -. , -. .6.2 . (7.3)
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