1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) ISO 22118 2013 () (ISO 22118:2011, IDT) 2014 ISO 22118-2013 II , 1.0 92 . 1.22009 . , . , , , 1 - ( ) 2 3 , ( 14 2013 . 44-2013) : ( 3166) 00497 ( 3166) 00497 AM BY KG MD RU UZ - 4 ISO 22118:2011 Microbiology of food and
2、 animal feeding stuff. Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens. Performance characteristics ( . () . ). CEN/TC 275 Food analysis - Horizontal methods ISO TC 34/SC 9 Microbiology ISO/TC 34 Food products (ISO). (en). , , . . (IDT) 5 22 2013 . 2074-
3、ISO 22118-2013 1 2015 . 6 ISO 22118-2013 III , . () . , , 2014 , ISO 22118-2013 IV . . , , () , . , , . ISO 22118-2013 1 () Microbiology of food and animal feeding stuff. Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens. Performance characteristics 2015 -
4、07 - 01 1 ( ) . , , (). . . 2 . : ISO 16140:2003 Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods ( - ) ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General req
5、uirements and definitions ( () ) 3 : 3.1 : , . : 1. , . 2. ISO 16140, 3.4. 3.2 : , , . 3.3 : , , ( ) ( , .) . ISO 16140, 3.6. ISO 22118 2013 2 3.4 : ( ) , . 3.5 : ( ) ( ) ( ). 3.6 : ( ) , , . 3.7 : . ISO 22174, 3.6.4 3.8 : . 1, 3.3.3. 3.9 (LOD): , , . ISO 22174, 3.1.8. 3.10 (LOQ): ( ) ( ), , . ISO 1
6、6140, 6.2.2.2.3. 3.11 : , . : 1 . 2 . . 3 . . 1, 3.3.4. 4 4.1 , . , , (, , ). 4.2 , . , , . 4.3 . 4.4 4.4.1 4.4.1.1 . . ISO 22118-2013 34.4.1.2 , . 50 . 4.4.2 4.4.2.1 . , . . 4.4.2.2 - 30 , , , . . , , . - , , 90 % . , , , 106 . , , , , , 4.4.2.3 - 30 . - 90 % . . , , . , , 106. , , , , , . 4.4.2.4 - - , , . , , 106. , , , , , . 4.5 4.5.1 , . 4.5.2 , , 1 10 , , 10 100 , . ( ). . , . , , . , , . . 4.5.3 .
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