GOST ISO 29981-2013 Milk products Enumeration of presumptive bifidobacteria Colony count technique at 37 C《奶制品 假定双歧杆菌的枚举 37 ℃时的菌落计数技术》.pdf

1、 37 (ISO 29981:2010, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)ISO 299812013, - 1.092 . - 1.22009 . , . , , -, 1 - , 42 ()3 , (- 5 2013 . 61-) : ( 3166) 00497 ( 3166) 00497 AZAMKZKGMDRUUZ -4 22 2013 . 1708- ISO 299812013 12015 .5 ISO 29981:2010 Milk products.En

2、umeration of presumptive bifidobacteria Colony count technique at 37 C ( . . 37 ). / 34 - (ISO). (en). , - , , , (-) . IDT6 - , - . () - . , , 2014 -, II ISO 2998120131 12 13 24 25 , 36 47 .58 59 710 811 1112 .11 () .12 () .13 14III ISO 299812013 . 37 Milk products. Enumeration of presumptive bifido

3、bacteria. Colony count technique at 37 C 201507011 - , 37 C . , , , , , - .( 6.), , (7, 8,16):a) Bifidobacterium adolescentis;b) B. animalis subsp. animalis;c) B. animalis subsp. lactis;d) B. bifidum;e) B. breve;f) B. infantis;g) B. longum.2 - . . -, .ISO 6887-1 Microbiology of food and animal feedi

4、ng stuffs - Preparation of test samples, initialsuspension and decimal dilutions for microbiological examination Part 1: General rules for the preparation ofthe initial suspension and decimal dilutions ( . , - . 1. )ISO 6887-5 Microbiology of food and animal feeding stuffs Preparation of test sample

5、s, initialsuspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation ofmilk and milk products ( . , . 5. )1 ISO 299812013 ISO 7218 Microbiology of food and animal feeding stuffs General requirements and guidance formicrobiological examinations ( . - )

6、ISO 7889|IDF 117 Yogurt. Enumeration of characteristic microorganisms. Colony-count technique at37 C (. . 37 C)ISO/TS 11133-1 Microbiology of food and animal feeding stuffs Guidelines on preparation andproduction of culture media Part 1: General guidelines on quality assurance for the preparation of

7、 culturemedia in the laboratory ( . - . 1. )ISO 14461-1|IDF 169-1 Milk and milk products Quality control in microbiological laboratories Part 1:Analyst performance assessment for colony counts ( . - . 1. )ISO 14461-2|IDF 169-2 Milk and milk products Quality control in microbiological laboratories Pa

8、rt 2: Determination of the reliability of colony counts of parallel plates and subsequent dilution steps ( . . 2. )3 :3.1 (bifidobacterium): , , 1 4 , , (TOS-MUP), , .4 4.1 , (MUP), , . MUP - (Streptococcus thermophilus, Lactobacillusdelbrueckii subsp. bulgaricus), (, Lactococcus lactis), Lactobacil

9、lusacidophilus, Lactobacillus casei Lactobacillus rhamnosus . - . TOS- , - 17.1 - 100- - , , , , , , (V- ), -, , - ().2 , , -, - , . -6- , -6- (F6PPK, EC 4.1.2.22) -6- -4-.3 37 C 41 C. 9.4.2 TOS- MUP, - , 37 C 72 .2 ISO 2998120134.3 14, 15.4.4 , , .5 , , , - , .5.1 ISO 6887-5 ISO/TS 11133-1.5.2 ISO

10、6887-5. (), . (Ringers) 1/4 -, ISO 6887-5 . . (22 c177 2) C. , . ISO 6887-1.5.3 ( TOS-MUP) - (TOS-MUP), .5.3.1 ( TOS- 10)5.3.1.1 : 10,0 ; 1,0 ;KH2PO4 3,0 ;K2HPO4 4,8 ;(NH4)2SO4 3,0 ;MgSO4c2157H2O 0,2 ;(R)- c215HCl c215H2O 0,5 ; 15,0 ;TOS (. 5.3.1.2) 10,0 ;- 15,0 ; 950 3.5.3.1.2 TOS , c98- Aspergillu

11、soryceae. TOS (Gal) (Glc), ()Gal Gal Glcn, (1) n =14;x = c98 1,6 c98 1,4 c98 1,3;y = c98 1,4 c98 1,3 c98 1,6. TOS 18, 19. - ( 97 % ) -, -, - .( .)5.3.1.3 950 3 (, - ) .3 ISO 299812013x y 250 3 190 3 . pH (6.6), , pH 6,3 c177 0,2 25 C. 115 C 15 . , . 2 C 4 C , . TOS , . TOS- , - . , - , . , , , , (. ISO/TS 11133-1).5.3.2 MUP 11 , , , 50 MUP 50 3 . (- 0,22 ) 5.3.3.5.3.3 190 3 (5.3.1) . (6.5) - (48 c177 1) C. 10 3 MUP (5.3.2) -, 0,22 (6.11) . , . (6.5) 48 C , . TOS-MUP MUP, 50 /3.6 , , , - , ISO 6887-5, ISO/TS 11133-1