GOST ISO TS 10272-2-2013 Microbiology of food and animal feeding stuffs Methods for detection and enumeration of Campylobacter spp Part 2 Colony-count technique《食品和动物饲料的微生物学 弯曲杆菌属检.pdf

1、 Campylobacter spp. 2 (ISO/TS 10272-2:2006, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC) ISO/TS10272-22013, - 1.092 . 1.22009 . -, . , , 1 - ( )2 ( 335)3 , ( 7 2013 . 43) : ( 3166) 00497 ( 3166) 00497 KZKGRUTJUZ 4 27 2013 . 228- ISO/TS 10272-22013 - 1 2014 .5 ISO

2、/TS 10272-2:2006 Microbiologyof food and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp. Part 2: Colony-count technique ( . - Campylobacter spp. 2. ) SC 9 - ISO/TC 34 (ISO). (en). , - , , , (-) . (IDT). 539932010/ISO/TS 10272-2:20066 II ISO/TS 10272-22013

3、-, - . () . , - - , 2014 -, III ISO/TS 10272-220131 .12 13 24 .24.1 24.2 25 26 .47 .58 59 .510 610.1 Campylobacter 610.2 .610.3 8 A () .9 () 1011IV ISO/TS 10272-22013 Campylobacter spp. 2 Microbiology of food and animal feeding stuffs. Methods for detection and enumeration of Campylobacter spp. Part

4、 2.Colony-count technique1 Campylobacter spp. :- ;- .2 :ISO 6887 ( ) Microbiology of food and animal feeding stuffs Preparation of test samples,initial suspension and decimal dilutions for microbiological examination ( . , - )ISO 7218 Microbiology of food and animal feeding stuffs General requiremen

5、ts and guidance formicrobiological examinations ( . - )ISO 8261 Milk and milk products. General guidance for the preparation of tests samples, initialsuspensions and decimal dilutions for microbiological examination ( . , )ISO/TS 11133-1 Microbiology of food and animal feeding stuffs Guidelines on p

6、reparation andproduction of culture media Part 1: General guidelines on quality assurance for the preparation of culturemedia in the laboratory ( . - . 1. )ISO/TS 11133-2 Microbiology of food and animal feeding stuffs Guidelines on preparation andproduction of culture media Part 2: Practical guideli

7、nes on performance testing of culture media (- . - . 2: )1 ISO/TS 10272-22013 201407013 :3.1 Campylobacter(Campylobacter):,- , 41,5 C, - 25 C, , , . Campylobacter jejuni Campylobacter coli. , (Campylobacter lari, Campylobacter upsaliensis - ).3.2 Campylobacter (count of Campylobacter): Campylobacter

8、, - , .4 4.1 ISO 6887 ISO 8261.4.2 , , ( mCCD), , - . , - . 41,5 C 4048 ., Campylobacter, , , - . Campylobacter 1 3 - .5 5.1 , Campylobacter ISO 7218, ISO/TS 11133-1 ISO/TS 11133-2.5.2 ISO 6887.5.3 , ( mCCD)5.3.1 5.3.1.1 : 10,0 ; 10,0 ; 5,0 ; 4,0 ; 3,0 ; 1,0 ; (II) 0,25 ; 0,25 ; 8,018,0 1); 1000 3.1

9、) .2 ISO/TS 10272-220135.3.1.2 , . pH , (7,4 c177 0,2) - 25 C. . (6.1) 121 C 15 .5.3.2 5.3.2.1 : 0,032 ; B 0,01 ; 5 3.5.3.2.2 . .5.3.3 5.3.3.1 : (5.3.1) 1000 3; (5.3.2) 5 3.5.3.3.2 , (47 c177 2) C, . 15 3 (6.8). . , , (6.2) 30 , . -, 4 - (5 c177 3) C .5.3.3.3 ISO 11133-1. IS 11133-2, B.5.5.4 5.4.1 5

10、.4.1.1 : 23,0 ; 1,0 ; 5,0 ; 8,018,0 1); 1 000 3.5.4.1.2 , . -pH,(7,3 c177 0,2).pH25 C. . (6.1) 121 C 15 .5.4.2 .5.4.3 5.4.3.1 : (5.4.1) 1000 3; (5.4.2) 50 3.5.4.3.2 , , (47 c177 2) C, . 15 3 (6.8). . , , (6.2) 30 , . -, 4 (5c177 3) C.1) .3 ISO/TS 10272-220135.4.3.3 ISO/TS 11133-1. ISO/TS 11133-2. C.

11、 coli ATCC 43478 C. jejuniATCC 33291 24 37 C.5.5 5.5.1 : 10,0 ; 10,0 ; 1,0 ; 2,0 ; 5,0 ; 0,1 ; 1 000 3.5.5.2 , - . pH , (7,0 c177 0,2).pH25 C.103. (6.1) 121 C 15 .5.5.3 ISO/TS11133-1. - ISO/TS 11133-2, B.4.5.6 5.6.1 :N,N,Nc162,Nc162-1,4-, 1,0 ; 100 3.5.6.2 .6 7218 , , -.6.1 ( ) (-) ISO 7218.6.2 , , 37 C 55 C.6.3 , (41,5 c177 1) C.6.4 , (25 c177 1) C.6.5 , 44 C47 C.6.6 pH- 0,1 . pH 25 C.6.7 , , , - .6.8 , , , 90100 .6.9 , , - 1 3103, 0,1 3.6.10 , .6.11 -, - - 3 . - (. 9.4.3).6.12 , .4 ISO/TS 10272-220136.13 , ( - Campylobacter).6.14 (5 c177 2) %, (10 c177 3) %, c16310