GOST R 54946-2012 Honey Method for determination of ethanol content《蜂蜜 乙醇的测定方法》.pdf

1、 549462012 DIN 10762:2004-06(NEQ) 27 2002 . 184- , 1.02004 . 1 - ( )2 432 3 31 2012 . 208-4 DIN 10762:2004-06 . . - (DIN 10762:2004-06 Analysis of honey Determination of ethanol content Enzymaticmethod) ( . 4709/DIN 31.05.2010 .)5 , - . () . - , , 2013 , - - II 5494620121 .12 13 24 .25 26 .27 .28 ,

2、, 39 410 411 512 .6III 549462012 Honey. Method for determination of ethanol content 201307011 - 4,00 155,00 /. 52451, 54644.2 : 5725-12002 ( ) . 1. 5725-62002 ( ) . 6. 5156899 ( 3310-190) . 520012002 . 524512005 . 525012005 ( 3696:1987) . 532282008 . 1. . 546442011 . 12.1.00491 . . 12.1.00776 . . 12

3、.1.01979 . . - 12.4.00983 . . . 12.4.02175 . . 34277 . 10-. 177074 ( 104283, 478880) ., , , . 311877 ( 427683) . . 376978 . . 417477 . 7-. 420775 . 3-. 1 549462012 1202676 . 2533682 . , 2849890 . . 2922791 ( 835-181) . . 1. - - , 1 , , . (), - () . , , , , .3 52001, 5725-1.4 12.1.004, 12.1.019, 12.1

4、.007, 12.4.009.5 , -, , 1- 2- , .12.4.021. : 1525; 25 80 %; 97101(730760 . .).6 200 54644.8.3 40 . . 8.6. - 8.7. . ( ) . 3 . - , .7 , , - (), - .2 549462012 7.1 - () - (ADH) + +ADHc190c174c190c190 + + +. .7.2 (Al-DH) + +H2OAl DHc45c190c174c190c190 + + +7.3 .8 , , 8.1 , 340 10 .8.2 53228 c177 0,01 .8

5、.3 , , 40 .8.4 pH-/ pX (pH) c177 0,02 . pX (pH).8.5 -26-2-000.8.6 , 0,5 51568.8.7 .8.8 0 100 1 28498.8.9 1(2)-100-2() 1770.8.10 -1(2,3)-25(250)-14/23(29/32) 25336.8.11 1-2-1-1(5, 10) 29227.8.12 (550) .8.13 3-100-2 1770.8.14 -1-100 25336.8.15 -75-110 25336.8.16 12026, - ( ).8.17 15 20 .8.18 3- 4207,

6、. .8.19 7- 4174, . .8.20 10- 342, . . .8.21 3118, . ., 1,0 /3.8.22 3769, ., 3,2 /3.8.23 c98- 4- 98,0 %.8.24 , 15 / .8.25 , 300 / .8.26 52501, 2. -, . .3 5494620129 9.1 I 4207 (3,60 c177 0,01) 8.26 100 3 1770. ,. 4 1 .9.2 II 4174 (7,20 c177 0,01) 8.26 100 3 1770. , . 6 .9.3 342 (4,50 c177 0,01) 60 3

7、8.26 100 3 1770. - 1,0 /3 8.21 , pH- 8.4 pH (9,0 c177 0,1) . pH. , . 4 2 .9.4 c98- 4- 8.23 (0,1140 c177 0,0001) 8.24 (0,0033 c177 0,0001) , 9.3, 100 3 1770. , . 4 .9.5 8.25 (0,7500 c177 0,0001) 3,2 /3 8.22 25 3 1770. , -. 4 4 .9.6 100 3 25336 (20,000 c177 0,001) , 6. 1020 3 8.26, 100 3 1770. - - , - 8.26, , 2/3 . 1 3 I, - 9.1, II, 9.2, . - 8.26, 24 . - 250 3 25336. . .10 10.1 25 3 25336 3 3, 9.4. 0,50 3 , 9.6, 0,50 3 8.26. , . .4 54946201210.2 3 (D1) (. 10.1) 8.1 - c108= 340 .10.3 0,05 3 , 9.5, , . 510 (D2)