GOST R EN 14130-2010 Foodstuffs Determination of vitamin C by HPLC《食品 维生素C的HPLC测定法》.pdf

1、 EN 14130:2003Foodstuffs Determination of vitamin C by HPLC(IDT) 141302010 27 2002 . 184- , 1.02004 . 1 - ( ) - , 42 93 , 3 26 2010 . 542-4 14130:2003 - . . (EN 14130:2003 Foodstuffs Determination of vitamin C by HPLC). - 1.5 ( 3.5). - , 5 - , - . () . - , , 2012 , - - II 1413020101 12 13 14 15 .36

2、37 58 .59 .6 () .7 B () .8 () 9 10 141302010III Foodstuffs. Determination of vitamin C by HPLC 201201011 - (). - L(+)- L(+)- .2 : 3696 . ( 3696:1987)3 , - L(+)- L(+)- - L(+)- () 265 1, 2.4 4.1 , , - - 3696 , .4.2 4.2.1 (HPO3)n.4.2.2 () w (Na3PO4c215 12H2O) c179 98,0 %.4.2.3 w (KH2PO4) c179 98,0 %.4.

3、2.4 L- w (C3H7NO2S) c17999,0 % - .4.2.5 N-N,N,N- () w (C19H42BrN) c17999,0 %.4.2.6 w (CH4O) c179 99,9 %.1 1413020104.2.7 c114 (HPO3)n) = 200 /3200 (. 4.2.1) 1 3, . 1 4 .4.2.8 c114 (HPO3)n) = 20 /350 3 (. 4.2.7) 500 3, . .4.2.9 c114 (Na3PO4c215 12H2O) = 200 /3200 (. 4.2.2) 1 3, .4.2.10 L- c114 (C3H7N

4、O2S) = 40 /320 (. 4.2.4) 500 3, - . .4.2.11 13,6 (. 4.2.3) 900 3. 0,45 . 100 3 (. 4.2.5) 100 3 (. 4.2.6). - 0,45 . , 900 3 100 3 . - .4.2.12 ( ), 1 /100 3.4.2.13 ( ), (I2) = 0,05 /3.4.2.14 , (H2SO4) = 0,1 /3.4.3 4.3.1 (L(+)-) w (C6H8O6) c179 99,0 %(R)-5-(S)-1,2-3,4-5-2-.4.3.2 (D()-) = w(C6H8O6) c179

5、 99,0 %(R)-5-(R)-1,2-3,4-5-2-.4.4 4.4.1 c114 (C6H8O6) = 1 /3100 (. 4.3.1), 0,1 , - (, 100 3) (. 4.2.8). . L-.4.4.2 - c114 (C6H8O6) = 1 /3100 (. 4.3.2), 0,1 , - (, 100 ) (. 4.2.8). .4.4.3 ( ) 150 , 10 3 (. 4.2.14) 80 3, , - . (. 4.2.12) (. 4.2.13) . , 1 3 , , 8,81 .2 141302010 wst, %, wst=Vm1881 100c

6、215c215, (1) V1 , , 3;8,81 ;100 ;m , .4.5 4.5.1 c114 (C6H8O6)=550/3 0,5 5,0 3 (. 4.4.1) - 100 3, - (. 4.2.8). .4.5.2 c114 (C6H8O6) = 10 /313 (. 4.4.2) 100 3, (. 4.2.8). .5 5.1 , - , .5.2 , - .5.3 , , , -, 265 , , .5.4 -5.4.1 L- 3.5.4.2 4,0 , 250 , - - 5 . , , .5.5 , , 0,2 0,4 , .6 6.1 - . 25 . - 8 .

7、3 1413020106.2 . -, . . (. 6.3.1).6.3 6.3.1 , , 3 ( 50 /100 ), - 1 , 100 3, 80 3- (. 4.2.8), , . , . - . 2 10 100 3, (. 4.2.8). , - 100 3, . . , , - 125 3 L- (. 4.2.10), - . , . -.6.3.2 (. 6.3.1) 20 3 - 50 3, 10 3 L(+)-. 7,07,2 - (. 4.2.9) . 5 . - 2,52,8 ( -) - (. 4.2.7) . 50 3, -, , , , . , . (. 5.5), . - - - . 4 3 1 3 (. 4.2.6), (. 5.5). .6.4 L- , . - . ,