KS H ISO 10520-2008 Native starch－Determination of starch content－Ewers polarimetric method《本地淀粉 淀粉含量的测定 旋光法》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS H ISO 10520 Ewers KS H ISO 10520:2008 2008 11 7 http:/www.kats.go.krKS H ISO 10520:2008 : ( ) ( ) KT&G ( ) : (http:/www.standard.go.kr) ： ：2003 11 28 ：2008 11 7 2008-0741 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS H ISO 10520:2008 Ewers Native s

2、tarch Determination of starch content Ewers polarimetric method 1997 1 ISO 10520, Native Starch Determination of starch contentEwers polarimetric method , . ISO 10520 ISO/TC 93 . A B . 1 ( ) . ( ) . 2 . . ( ) . KS H ISO 1666, KS M ISO 3696, 3 . 3.1 . 3.2 40 %(V/V) . 3.1 . 3.1 3.2 . , . KS H ISO 1052

3、0:2008 2 . 4 , KS M ISO 3696 2 . 4.1 c(HCl) 7.7 mol/L 100 mL (20 1.19 g/mL) 63.7 mL . 4.2 c(HCl) 0.309 mol/L 1 000 mL (20 1.19 g/mL) 25.6 mL . . 10 mL HCl 0.1 mol/L 30.94 mL NaOH . 4.3 40 %(V/V) (20 0.948 g/mL) 4.4 Carrez potassium hexacyanoferrate(II) trihydrateK4Fe(CN)6 3H2O 10.6 g . 100 mL . 4.5

4、Carrez zinc acetate dihydrate Zn(CH3COO)2 2H2O 21.9 g 3 g . 100 mL . 5 . 5.1 100 mL . 5.2 5.3 589.3 nm 200 mm . KS H ISO 10520:2008 3 5.4 0.001 g . 6 0.5 mm 0.5 mm . . 7 0.001g (5.4 ). 7.1 7.1.1 (2.50.05) g (m1) (5.1) . (4.2) 25 mL . (4.2) 25 mL . 7.1.2 (5.2) . 7.1.3 15 5 . 30 mL (202) . 7.1.4 Carre

5、z I(4.4) 5 mL 1 . 7.1.5 Carrez II(4.5) 5 mL 1 . 7.1.6 . . Carrez 10 mL . 7.1.7 (5.3) 200 mm (1) . 7.2 40 %(V/V) 7.2.1 (50.1) g (m2) 100 mL (5.1) . (4.3) 80 mL . 1 . 10 . (4.3) 100 mL . 7.2.2 (5.1) ( 2.5 g ) 50 mL . (4.1) 2.1 mL . . 15 5 . KS H ISO 10520:2008 4 (202 ) . 7.2.3 7.1.4, 7.1.5 Carrez I II

6、 7.1.6 . 7.2.4 7.1.7 (2) . 7.3 KS H ISO 1666 w0 . w1 . w1 100 w08 w . 12211201005520002wmm.wD= 1 ： 7.1 () 2 ： 7.2 () m1 ： 7.1.1 (g) m2 ： 7.2.1 (g) w1 ： 7.3 ( ) 20D ： 589.3 nm (pure starch) ()( 1 ) 1 () 20D () 185.9 185.7 184.6 182.7 181.5 181.3 184.0 . 9 1990 KS A ISO 57251 ISO/TC 93/WG 1, . 12 . ,

7、. A . KS H ISO 10520:2008 5 9.1 2 2 . 9.2 2 2 . 2 r %(m/m) R %(m/m) 2.2 1.0 2.0 1.4 4.8 7.7 3.5 8.2 10 . . KS H ISO 10520:2008 6 A ( ) Ewers 1990 A.1 (RSDR) Pocklington4 . 3 000 ( ) Horwitz . RSDR 2(1 0.5 lg c) c： c 1 , RSDR 2 % RSDR 1 4 , . RSDr RSDR 1/2 2/3 . A.1 RSDR 1.25 % 3.24 % . A.1 95/ 5 90/

8、 10 85/ 15 12 11 12 11 11 12 12 1 1 1 24 22 24 22 22 24 24 % (m/m) 98.2 100.0 99.0 100.0 92.7 88.9 82.2 % (m/m) 98.9 99.7 99.0 99.7 93.3 89.1 85.0 , sr % (m/m) 0.79 0.35 0.71 0.48 0.63 0.55 1.54 (%) 0.80 0.30 0.71 0.48 0.68 0.62 1.87 , r 2.8sr % (m/m) 2.22 0.98 2.00 1.37 1.78 1.57 4.34 , sR % (m/m)

9、1.69 2.73 1.24 2.90 1.17 1.95 2.66 (%) 1.71 2.73 1.25 2.90 1.27 2.19 3.24 , R 2.8sR % (m/m) 4.77 7.72 3.50 8.20 3.33 5.52 7.53 KS H ISO 10520:2008 7 B ( ) 1 KS A ISO 5725( ), ( ) 2 KS A ISO 5725 1, ( ) 1： 3 KS A ISO 5725 2, ( ) 2： 4 International Union of Pure and Applied Chemistry, Harmonized proto

10、cols for the adoption of standardized analytical methods and for the presentation of their performance characteristics. J. Pure Appl. Chem., 62, No 1, 1990, p.152 Ewers 135513 7017 (02)60094114 (02)600948878 http:/ KS H ISO 10520:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Native starch Determination of starch content Ewers polarimetric method ICS 67.180.20 Korean Agency for Technology and Standards http:/www.kats.go.kr