KS H ISO 2881-2009 Tobacco and tobacco products－Determination of alkaloid content－Spectrometric method《烟草和烟草制品 碱含量测定 光谱法》.pdf

1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS H ISO 2881 KS H ISO 2881 :2009 2009 10 29 http:/www.kats.go.krKS H ISO 2881:2009 : ( ) ( ) () () () ( ) : () KT&G ( ) () () KT&G () () ( ) KT&G : (http:/www.standard.go.kr) ： ： 2004 9 7 ： 2009 10 29 2009-0664 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10

2、 5 , . KS H ISO 2881:2009 Tobacco and tobacco productsDetermination of alkaloid contentSpectrometric method 1992 3 ISO 2881, Tobacco and tobacco productsDetermination of alkaloid contentSpectrometric method , . 1 (alkaloid) . , . 2 . . ( ) . KS H ISO 3401, KS H ISO 4874, KS H ISO 6488, KS H ISO 8243

3、, ISO 565, Test sievesMetal wire cloth, perforated metal plate and electroformed sheetNominal sizes of openings 3 , (%) . 4 . KS H ISO 2881:2009 2 4.1 4.2 , c(NaOH)8 mol/L 4.3 , c(H 2 SO 4 )1 mol/L 4.4 , c(H 2 SO 4 )0.025 mol/L 4.5 , 98 % 5 . 5.1 KS H ISO 3401 (4.5) (7. ) . 98 % . . (4.5) (nicotine

4、hydrogen tartrate) . 5.2 (spectrometer) (230290) nm . 5.3 (matched quartz cells) (optical path length) 1 cm . 5.4 ISO 565 500 m . 5.5 . 5.6 5.7 250 mL , . KS H ISO 2881:2009 3 5.8 100 mL , . 5.9 (onemark pipettes) 5 mL, 10 mL, 25 mL 5.10 55 mm . 5.11 6 KS H ISO 4874, KS H ISO 8243 . 7 7.1 , (6.) . .

5、 . . (5.6) 40 , 500 m (5.4) . 2 . . . 7.2 KS H ISO 3401 . . 7.2.1 KS H ISO 2881:2009 4 . 7.2.2 (12) g( ) 0.001 g . 7.2.3 KS H ISO 6488 . 7.2.4 7.2.4.1 . (7.2.2) (chamber) (515) mL . (2040) g (4.1) (4.2) 5 mL (inlet funnel) . 1 . 10 mL (4.3) 250 mL . (1012) mL . . . (220 230) mL (delivery tube) . . (

6、 V 1 ) . , . . 2 , 150 mL . 7.2.4.2 ( ) ( V 2 , 25 mL) ( V 3 , 100 mL) (4.4) . (5.2) 236 nm, 259 nm 282 nm (4.3) 10 mL 250 mL . 259 nm 0.7 , ( V 2 ) (V 3 ) (4.4) , . KS H ISO 2881:2009 5 8 8.1 A . ) 2 ( 059 . 1 282 236 259 A A A A (1) A 236 , A 259 A 282 ：236 nm, 259 nm 282 nm 9. g , ( ) , w nic . )

7、 100 100 ( 100 2 3 1 nic b lm aV V AV w (2) a ： 0.025 mol/L (decadic extinction coefficient). , 259 nm 34.3 A ： B ： , (7.2.3 ). l ： (cm) m ： (mg) V 1 ： (mL) V 2 ：2 (mL) V 3 ： (mL) . al A c (3) cl A a (4) a 259 34.3 Q mg , . l aV V AV Q 2 3 1 (5) c , mg/mL , (2) . 8.2 (repeatability) 0.05 % , . KS H

8、ISO 2881:2009 6 9 . a) b) c) d) e) f) , 0.5 % . g) ( ). 0.01 % . h) . 0.01 % . 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KS H ISO 2881 :2009 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Tobacco and tobacco products Determination of alkaloid content Spectrometric method ICS 65.160 Korean Agency for Technology and Standards http:/www.kats.go.kr