KS H ISO 6800-2003 Animal and vegetable fats and oils－Determination of the composition of fatty acids in the 2－position of the triglyceride molecules 《动植物油脂 两种状态三酸甘油酯中脂肪酸成分的测定》.pdf

1、 2003 6 30 H ISO 6800 2003 ( ) ( ) ( ) ( ) F&B ( ) ( ) 2003 6 30 03 742 ( ) ( 02 509 7262 5) . 7 5 , . ICS 67.200.10 KS H ISO 6800 2003 Animal and vegetable fats and oils Determination of the composition of fatty acids in the 2 position of the triglyceride molecules 1997 ISO 6800 Animal and vegetabl

2、e fats and oils Determination of the composition of fatty acids in the 2 position of the triglyceride molecules . ISO 6800 ISO/TC 34 (SC 11) . , . 2 ( ) . 45 . . 12 ( , , ) 20 ( , ) 2 (n 16) (n 11) (petroselinic acid) . . . KS H ISO 660 2002 KS H ISO 661 2002 KS H ISO 5508 2003 KS H ISO 5509 2003 IS

3、O 3696 1987 Water for analytical laboratory use Specification and test methods . 2 . (TLC) . H ISO 6800 2003 . ISO 3696 2 . 95 %(V/V) 100 g 1 g , Brockmann I, 2 260 . . 6 mol/L 1 g/L 220 g/L 1 mol/L, 2 2 ( ) 1,3 ( 1 ) 2 amino 2 (hydroxymethyl) propan 1.3 diol, pH ( ) pH 8 . 0 4 14 . ( 1 ) . ( ) tris

4、(hydroxymethyl)methylamine ( ) tris(hydroxymethyl)aminomethane. 8 20 units/mg . . . . . ( ) 70 mL 3 0 mL 98%(V/V) 1 mL , 2 g/L, 100 mL 1 mol/L . KS H ISO 5508 KS H ISO 5509 . 2 H ISO 6800 2003 . 30 40 . 13 mm 400 mm . 250 mL . 500 mL . 100 mL . 10 mL . . 40 0.5 . 1 mL . , 200 mm 200 mm . . 200 mm 20

5、0 mm 3 4L . 103 2 . , 254 nm . 25 mL 1 mL . 250 mL . 5 0 mL ( ). P 40(16 40 m) ( ). . KS H ISO 5508 KS H ISO 5509 . . , KS H ISO 5555 1 . KS H ISO 661 . 3 H ISO 6800 2003 ( ) 2 . KS H ISO 660 . 3 %(m/m) . 3 %(m/m) . 10 g 100 mL ( ) ( ) ( ) . 50 mL 2 ( ) ( ) 0.5 % . 1 50 mL . ( ) . 2 ( ) . ( ) . 30 4

6、0 . 50 mL ( ) ( ) 15 g ( ) . 1 2 mm . 5 g 25 mL ( ) ( ) 100 mL . 30 40 . 10 mL ( ) ( ) 0.1 g . 60 65 . 10 . . 20 mg ( ) 2 mL ( ) . 0.5 mL ( ) 0.2 mL ( ) . 60 2 40 . 30 . ( ) 120 2 40 . 1 mL ( ) 1 mL ( ) . (organic phase) ( ) . 1 mL . . ( ) ( ), ( ) ( ) . 30 g ( ) 250 mL ( ) . 60 mL . 4 H ISO 6800 20

7、03 1 . ( ) . 0.25 mm . 1 . 103 1 . ( ) . (acyl) . TLC . . . 15 mm ( ) ( ) . ( ) . 10 mm . 20 . 20 ( ) ( ) . ( R f 0.035) ( ) . . ( ) 15 mL 50 mL ( ) . ( ) 15 mL 50 mL ( ) . 15 mL 3 . 4 5 mL 25 mL ( ) . 10 30 % (m/m) . ( ) ( ) . (boron trifluoride method) KS H ISO 5509 . KS H ISO 5508 . 2 2 . . . . 2 . 5 %(m/m) 0.2 %(m/m) 5 %(m/m) 1 %(m/m) 3 %(m/m) 5 H ISO 6800 2003 2 . 5 %(m/m) 0.5 %(m/m) 5%(m/m)