1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 14240 2 2: KS I ISO 14240 2:2009 2009 12 18 http:/www.kats.go.krKS I ISO 14240 2:2009 : ( ) ( ) () ( ) : (ISO/TC190) () ( ) () : (http:/www.standard.go.kr) : :1999 12 28 :2009 12 18 2009-0830 : : (ISO/TC190) ( 02-509-7266) (http:/www.kats.go.kr). 10
2、 5 , . KS I ISO 14240 2:2009 2: Soil quality Determination of soil microbial biomass Part 2: Fumigation-extraction method 1997 1 ISO 14240 2, Soil quality Determination of soil microbial biomass Part 2: Fumigation-extraction method . 1 . , . (FE) pH ( ) . . . 5 % . 2 . . ( ) . KS I ISO 10381 6, ( ,
3、) KS I ISO 10694, ( ) KS I ISO 11465, ( ) 3 . 3.1 KS I ISO 14240 2:2009 2 ( ) . , . 4 ( 1 ) . . 5 5.1 , , (KS I ISO 10381 6) . 40 % (Water Holding Capacity: WHC) . A . 30 % . (smearing) . . 5.2 . 5.2.1 ( ) 5.2.2 (COCl2) . 5.2.3 c(K2SO4) 0.5 mol/L( 87.135 g/L) 5.2.4 6 6.1 (252) 6.2 (Implosion-protect
4、ed) KS I ISO 14240 2:2009 3 6.3 1)6.4 6.5 6.6 250 mL . 6.7 ( ) 6.8 (horizontal or overhead shaker) 6.9 15 20 . 6.10 (Antibumping granules) 7 7.1 (6.2) (6.3) . 3 (5.1) 25 g 50 g , (6.4) (6.5) . (5.2.2) 25 mL (6.10) 1 (5.2.4) 1 . 2 . 22 24 (252) (6.1) . , . (1: 4). 20 % (KS I ISO 10694 ) 1: 4 ( 95 % ,
5、 L 1: 30 ). . . (2 6 ) . 3 ( 50 g) (6.6) 7.2 (5.2.3) 200 mL . 7.2 (6.6) (5.2.3) 200 mL1) Whatman No. 42, Schleicher & Schll 595 1/2 Machery & Nagel 261 G 1/4 . , KS . KS I ISO 14240 2:2009 4 30 200 rpm (horizontal shaker)(6.8) 45 60 rpm (overhead shaker) (6.3) . . ( 15 20) (6.9) . . 1 ( ) (CaSO4) .
6、, CaSO4 . 2 . B . 8 , . , . . (8.1) (8.2) . 8.1 8.1.1 6 3 . . 8.1.2 8.1.2.1 c(K2Cr2O7) 0.066 7 mol/L( 1 L 19.612 5 g) . 8.1.2.2 (H3PO4) 1.71 g/mL 8.1.2.3 (H2SO4) 1.84 g/mL 8.1.2.4 2 , c(NH4)2Fe(SO4)2 6H2O 0.040 mol/L KS I ISO 14240 2:2009 5 2 (15.69 g) (8.1.2.3) 20 mL , 1 000 mL . 8.1.2.5 1,10- 0.02
7、5 mol/L 8.1.2.6 (8.1.2.3) 2 (8.1.2.2) 1 . 8.1.3 8.1.3.1 8.1.3.2 250 mL 8.1.3.3 0.05 mL 10 mL . 8.1.3.4 2 mL . 8.1.4 250 mL (8.1.3.2) (7.1)(PS) 8 mL (8.1.3.4) (8.1.2.1)(PD) 2 mL (8.1.2.6) 15 mL . 30 (8.1.3.1) , 20 mL 25 mL . (5.2.3) 8 mL 2 . . 1,10- 2 (8.1.2.5) 2 (8.1.2.4) (8.1.3.3) . 8.1.5 (1) (2) .
8、 SDCSH0001g/mL)(PEMPVVVC (1) VS : (8.1.2.4) (mL) VH : (8.1.2.4) (mL) VC : (8.1.2.4) (mL) M : (mol/L) PD : (mL) KS I ISO 14240 2:2009 6 PS : (mL) E : 3 (C) (C4+) C(g/g )WWKg/mL)( SDPC (2) PK : (g) DW : (KS I ISO 11465 )(g) SW : (KS I ISO 11465) )(g- /g- ) (3) BC . ECCCkEB (3) EC : ( ) ( ) kEC : 0.38
9、(12 ) kEC . 8.2 8.2.1 (K2S2O8) , (IR) (UV) . 8.2.2 8.2.2.1 (K2S2O8) 8.2.2.2 (8.1.2.2 ) 8.2.2.3 (NaPO3)n . 8.2.2.4 (8.2.2.1) 20 g 900 mL (8.2.2.2) pH 2 1 000 mL . 8.2.2.5 (8.2.2.3) 50 g 900 mL (8.2.2.2) pH 2 1 000 mL . 8.2.3 KS I ISO 14240 2:2009 7 8.2.3.1 2) 3) . 8.2.4 (7.1) 5 mL (8.2.2.5) 5 mL . .
10、(8.2.2.4) (chamber) . . 8.2.5 (4) (C) . C(g/g ) WWKBV)()( SDPDBDV (4) V : (g/mL) B : (g/mL) DV : (mL) DB : (mL) PK : (2) DW : (2) SW : (2) (5) BC . ECCCkEB (5) EC : ( ) ( ) kEC : 0.45 1 (23 ) kEC . 2 (14C) . 9 C . . 2) Dohrman DC 80, Maihak Tocor 4 . , KS . 3) Skalar, Perstorp Alpkem . , KS . KS I I
11、SO 14240 2:2009 8 A ( ) A.1 (WHC) . A.2 . 105 . A.3 A.3.1 50 mm 150 mm, 50 mm 100 mm . A.3.2 ( ) A.3.3 20 mm 50 mm . A.3.4 (1052) . A.3.5 0.01 g . A.4 (A.3.1) . . 1 2 . (A.3.3) 2 24 . 105 . A.5 WHC(%) . KS I ISO 14240 2:2009 9 WHCDDTS 100 S : (g) T : ( )(g) D : (g) A.6 (WHC) . KS I ISO 14240 2:2009
12、10 B ( ) B.1 B.1.1 c(K2SO4) 0.05 mol/L( 8.714 g/L) B.2 B.2.1 B.3 ( 25 g 50 g) 250 mL 100 mL . 200 rpm 20 , ( 2 mm , 3 mm ) . ( ) (B.1.1) 75 mL . 15 500 g (B.2.1). . (5.2.2) 3 . 7.1 . B.4 . . . . KS I ISO 14240 2:2009 11 C ( ) 11 . 2 , . C.1 EC . EC . EC kEC . C.1 g/g CVa% SEb (loess soil) ECc71 207
13、136 21 15 23 6.7 7.4 9.2 (loam soil) ECc85 265 180 18 11 15 5.4 7.1 8.6 aCV bSE (m 4) c KS I ISO 14240 2:2009 12 D ( ) 1 KS I ISO 10390, pH 2 KS I ISO 11274, ( ) 2: 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KS I ISO 14240 2:2009 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Soil qualityDetermination of soil microbial biomass Part 2:Fumigation-extraction method ICS 13.080 Korean Agency for Technology and Standards http:/www.kats.go.kr
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