KS I ISO 15952-2007 Soil quality-Effects of pollutants on juvenile land snails(Helicidae)-Determination of the effects on growth by soil contamination《土壤质量 污染物对幼年陆生螺类的影响(庭园大蜗牛) 第2部.pdf

1、 KS I ISO 15952 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS (Helicidae) KS I ISO 15952 : 2007 (2012 ) 2007 11 7 http:/www.kats.go.krKS I ISO 15952：2007 : ( ) ( ) ( ) () ( ) E&M / : (http:/www.standard.go.kr) ： ：2007 11 7 ：2012 12 27 20120781 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO

2、 15952：2007 . iii 1 1 2 1 3 .2 4 3 5 3 6 .4 6.1 .4 6.2 (biological material) .4 6.3 (test substrate)4 6.4 (feed)5 7 .6 7.1 (test containers)6 7.2 (containers for food) .6 7.3 (calliper rule).6 7.4 .6 8 6 8.1 .6 8.2 (waste material)6 9 6 9.1 6 9.2 (distribution of the test mixture) .8 9.3 (introducti

3、on of the feed).9 9.4 (introduction of the biological reagent).9 9.5 9 10 (reference substance).10 11 11 11.1 .11 11.2 12 12 Helix aspersa aspersa 13 13 .13 i KS I ISO 15952：2007 A() (static method) 15 A.1 .15 A.2 .15 A.3 (reference substance) 15 B() (breeding technique).16 B.1 .16 B.2 .17 B.3 .18 B

4、.4 19 C() .22 D() .23 E() Helix aspersa aspersa 24 F() .27 F.1 27 F.2 .27 F.3 .27 F.4 28 F.5 30 F.6 30 G() 31 32 ii KS I ISO 15952：2007 iii . KS M ISO 15952 . A() B() C() D() E() Helix aspersa aspersa F() G() KS I ISO 15952 : 2007 (2012 ) (Helicidae) Soil quality Effects of pollutants on juvenile la

5、nd snails(Helicidae) Determination of the effects on growth by soil contamination 2006 1 ISO 15952, Soil qualityEffects of pollutants on juvenile land snails (Helicidae)Determination of the effects on growth by soil contamination , . 1 (Helix aspersa aspersa Mller) (semi-static) . ( ) . ( .) (). A .

6、 (H) / 1 0.013 3 Pa (25 ) . 28 7 , , . 2 . . ( ) . KS M ISO 103816, 6： , KS I ISO 15952：2007 KS M ISO 10390, pH KS M ISO 10694, () KS M ISO 112681, 1： KS M ISO 112692, 2： KS M ISO 11274, ( ) KS M ISO 11465, () EN 14753, 3 . 3.1 (test substrate) 3.2 (matrix) 3.3 (test mixture) , 3.4 (growth) , ( ) .

7、3.5 (effect concentration) EC x ：x (10, 25, 50). EC 50 50 % . 3.6 (median lethal concentration) LC 50 50 % 3.7 (lowest observed effect concentration) LOEC 2 KS I ISO 15952：2007 (p 0.05) 1 NOEC LOEC . 2 3.5, 3.6, 3.7 3.8 . 4 , 28 . 7 . (6.3.2) (6.3.3) . . ( ) 28 ( 28 7 ). NOEC LOEC EC 50,m (28) EC 50

8、,d(28) EC x 28 50 % . 50 % LC 50 (28) . (EC x , NOEC, LOEC, LC 50 ) 28 (7, 14, 21 ) . 2 . , NOEC . . 1090 % . . 5 18 6 (202) . ( 3 KS I ISO 15952：2007 ) 50 lux 100 lux . 6 6.1 6.2 (biological material) . Helix aspersa aspersa Mller 3 5 (10.3) g (15.51) mm . Helicidae ( G ). , , . B . (35, B ) 1 5 .

9、17 20 ( 12 cm, 4 cm). 2 3 (6.1) . 10 % . ( ) (6.1) (7.1) . (6.3) 50 % 60 % . (23) (6.4) . 6.3 (test substrate) 6.3.1 (6.3.2) (6.3.3) . . () ( .) . 6.3.2 (artificial soil) (KS M ISO 112681 ). 4 KS I ISO 15952：2007 1 (21) mm . 10 % (Kaolinite clay), 30 % 20 % ( 50 % 0.05 mm 0.2 mm) 69 %( CaCO 3 .) pH

10、6.00.5 (CaCO 3 , .) 0.3 % 1.0 % 2 . ( ) . 2 . pH 50 % 60 % 1 2 . pH KS M ISO 10390 . pH . KS M ISO 11274 KS M ISO 112692 A . 6.3.3 (natural soil) 4 mm (mesh) . pH：KS M ISO 10390 . ：KS M ISO 11274 KS M ISO 112692 A . ：KS M ISO 11465 . ：KS M ISO 10694 . KS M ISO 11260 . 6.4 (feed) 5 % 10 % . , , . 1)

11、C . 1) . “Helixal” by tablissements Chays Frres, 6, rue du Collge, BP 21, 25800 Valdahon, France INRA by tablissements Berton SARL, Lieu-dit Berton / Dpartementale 23, 85510 Le Boupre, France UCAAB rue de lglise, BP 19, 02400 Chteau-Thierry Cedex, France . , ISO . 5 KS I ISO 15952：2007 7 . 7.1 (test

12、 containers) 2)1 1.6 L ： 24 cm(), 10.5 cm(), 8 cm() 7.2 (containers for food) 5.5 cm 1 cm 7.3 (calliper rule) 0.1 mm . 7.4 1 mg 0.1 g 1 g 8 8.1 KS M ISO 103816 . 4 mm . (6.3.3) . 8.2 (waste material) EN 14735 (43) 2 . 4 mm . , 4 mm . 9 9.1 9.1.1 9.1.1.1 (preliminary test) 2) 1 Charles River Laborato

13、ries France, BP 0109, 69592 LArbresle Cedex, France E1DBBAC001 , , ISO . 6 KS I ISO 15952：2007 . 4 1 (： 0 mg/kg, 50 mg/kg, 100 mg/kg, 500 mg/kg, 1 000 mg/kg) , 1 1 5 . 4 1 (：0 %, 12,5 %, 25 %, 50 %, 100 %) , 1 1 5 . 9.1.1.2 (final test) , 5 , . 2 . 2 10 % 90 % 2 . 3 . 9.1.2 9.1.2.1 (3.3) , , . 1 cm

14、. ( ) (kg) (mg) . ( ) ( ) . 9.1.2.2 (6.1) . (6.3) . (KS M ISO 11274 KS M ISO 112692 A ) 50 % 60 % . KS M ISO 10390 pH . . 9.2 . 9.1.2.3 (： ) . (6.3) 24 . 7 KS I ISO 15952：2007 (6.1) (KS M ISO 11274 KS M ISO 112692 A ) 50 % 60 % . KS M ISO 10390 pH . . 9.2 . 9.1.2.4 (6.3.2) 10 g( .) . (6.3)( 10 g ) .

15、 (6.1) (KS M ISO 11274 KS M ISO 112692 A ) 50 % 60 % . KS M ISO 10390 pH . . 9.2 . 9.1.2.5 (solid matrixes) (6.3)(：0 %, 12,5 %, 25 %, 50 %, 100 %) . 0 % . (6.3.2) (6.3.3) 100 % . (6.1) (KS M ISO 11274 KS M ISO 112692 A ) 50 % 60 % ( ). 30 . KS M ISO 10390 pH . 9.2 . 9.2 (distribution of the test mix

16、ture) 1 cm (7.1) (9.1.2) . (6.3) 140 g( ) . . 8 KS I ISO 15952：2007 9.3 (introduction of the feed) (7.1) (6.4) (7.2) . . 9.4 (introduction of the biological reagent) (7.1) 5 (6.2) . 9.5 9.5.1 2 (Plexiglas) 26.5 cm13.5 cm . . 2 (7.1) . ( B.2 ). 2 mm 3 4 . (5.) . . 9.5.2 3 3 ( , , ) . . (6.1) . (6.1) . (6.1) (9.1.2) 50 % 60 % . 50 % 60 % . (6.4) . ( ) . . 9.5.3 7 ( , 4. ), . (28) . 7 ： 9 KS I ISO 15952：2007 0.1 g 0.1 mm ( 1 ). (9.1.2) . 1 , ( ). . . . ( , (浮腫) , 11 41 ), ( ) . pH KS M ISO 10390 . pH . ： (28) 48 ( ) . . 10 (reference substance) . (cadmium chloride)