KS I ISO 16000-17-2012 Indoor air－Part 17：Detection and enumeration of moulds－Culture-based method《室内空气 第17部 霉的检测与计数 培养法》.pdf

1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 16000 17 17： KS I ISO 16000 17:2012 2012 12 27 http:/www.kats.go.krKS I ISO 16000 17:2012 : ( ) ( ) ( ) : (ISO/TC146) () ( ) : (http:/www.standard.go.kr) ： ：2012 12 27 2012-0779 ： ： (ISO/TC146) ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I I

2、SO 16000 17:2012 i . ii 1 1 2 1 3 .1 4 2 5 3 6 .3 6.1 (dicloran) 18 % (DG18 ) 3 6.2 (malt-extract agar) 4 6.3 (potato dextrose agar).5 6.4 .5 6.5 80(polysorbate 80) 5 7 6 7.1 .6 7.2 6 7.3 .7 7.4 7 7.5 .8 7.6 .8 8 .11 A( ) 12 B( ) 16 21 KS I ISO 16000 17:2012 ii 2008 1 ISO 16000 17, Indoor air Part 1

3、7： Detection and enumeration of moulds Culture-based method , . KS I ISO 16000 17:2012 17： Indoor air Part 17： Detection and enumeration of mouldsCulture-based method , . . . 1 ISO 16000 18 KS I ISO 16000 16 . . 2 . . ( ) . KS I ISO 8199, KS I ISO 16000 16, 16： ISO 16000 18, Indoor air Part 18： Dete

4、ction and enumeration of moulds Sampling by impaction 3 . 3.1 (filamentous fungus) 1 . 2 “ ” . 3.2 (filtration) KS I ISO 16000 17:2012 2 . 3.3 (indirect mehtod) , 3.4 (colony forming unit) cfu 1 (cfu) , , . 2 . 3.5 (cultivation) . 3.6 (microorganism) 3.7 (moisture indicator) . 3.8 2 (secondary colon

5、y) “1 ” 3.9 (mould) , , , . 4 DG18 (malt-extract agar) (potato dextrose agar) (253) . 0.01 % 80(polysorbate 80)1) 1) 80(ploysorbate 80) (polyoxyethylenesorbitan monooleate) KS I ISO 16000 17:2012 3 (0.9 % NaCl) . 10 1 DG18 (malt-extract agar) (potato dextrose agar)( ) . (253) . (362) ( Aspergillus s

6、pp. ) (452) (Aspergillus fumigatus) . , . . 5 . 5.1 , , (253) 5.2 , , (362) 5.3 , (452) 5.4 , (53) 5.5 pH , 0.1 pH 5.6 , II 5.7 , 35 40 5.8 , (Vortex) 2) 5.9 (Petri dishes), 90 mm 5.10 , (1153) (1213) 6 ( ) , . . . 6.1 (dicloran) 18 % (DG18 ) 1 . (polyehtylene glycol sorbitan monooleate) . Tween . K

7、S . 2) . KS . . KS I ISO 16000 17:2012 4 1 (dicloran) 18 % (DG18 ) 3)(peptone) 5.0 g (glucose) 10.0 g (KH2PO4) 1.0 g (MgSO4 7H2O) 0.5 g 0.2 % (2,6-dichloro-4-nitroaniline) 1.0 mLa (chloramphenicol) 0.1 g (glycerol) 220 gb (agar) 15.0 g 1 000 mL a ： 0.002 g/L b 1 220 g 18 % 220 g 800 mL . 1 000 mL 22

8、0 g . (151) (1213) . pH 25 5.60.2 . (Petri dishes) 20 mL . (bags) DG18 (53) 1 . DG18 . . DG18 ( , ) . aH2O 0.95 . (chloramphenicol) . (dicloran) (colonies) (colonies) . 6.2 (malt-extract agar) 2 . 2 (malt-extract agar) (malt extract) 30.0 g (peptone from soy) 3.0 g (agar) 15.0 g 1 000 mL (chloramphe

9、nicol 0.05 g/L) . 3) (casein peptone, mycological peptone ) (peptone) . . (peptone) (colony) (positive control) . KS I ISO 16000 17:2012 5 . . (101) (1153) . , pH 25 5.50.2 . (Petri dishes) 20 mL . (bags) (malt-extract agar) (53) 1 . , . 6.3 (potato dextrose agar) 3 . 3 (potato dextrose agar) (potat

10、o extract) 4.0 g (glucose) 20.0 g (agar) 15.0 g 1 000 mL (chloramphenicol 0.05 g/L) . . (101) (1153) . , pH 25 5.60.2 . (Petri dishes) 20 mL . (bags) (potato dextrose agar) (53) 1 . 6.4 4 . 4 (NaCl) 8.5 g 1 000 mL NaCl (151) (1213) . 6.5 80(polysorbate 80) 5 . KS I ISO 16000 17:2012 6 5 80(polysorba

11、te 80) (NaCl) 8.5 g 80(polysorbate 80) 0.1 g 1 000 mLNaCl (151) (1213) . 80(polysorbate 80) . 7 7.1 ISO 16000 18 KS I ISO 16000 16 . . 7.1.1 (7.3 ). 7.1.2 (7.2 ) (7.3 ). 48 . ( 25 ) ( , , ) . . . . 7.2 7.2.1 . , (colonies) . 30 30 . , . 7.2.2 (5.6) 80(polysorbate 80) (6.5 ) 5 mL ( ) . KS I ISO 16000

12、 17:2012 7 35 40 15 (5.8). , . 1 7.2.3 . 7.2.3 . (5.8) 1 . 9 mL (6.4) 1 mL . 1： 10, 1： 100 1： 1 000 . . . 7.2.4 (7.2.3) 0.1 mL DG18 (6.1) (malt-extract agar)(6.2) (potato dextrose agar)(6.3) . 2 (7.3). , 1 mL 4 ( 250 L ) . . 7.3 (253) 7 . DG18 10 . ( , Aspergillus spp.) , (malt-extract agar plates)

13、(potato dextrose agar plates) (362) . Aspergillus fumigatus , (452) . 25 , . (bags) . . 2 (colony) . . 7.4 2 3 , 7 . (36 45 ) 1 3 . KS I ISO 16000 17:2012 8 2 (colony) . 2 (colony) . 90 mm 20 40 (colonies) . , / 10 (colonies) , 100 (colonies) . (dicloran) (colonies) (Rhizopus, Chrysonilia, Mucor, Bo

14、trytis ) (colonies) . ( ) ( ) 7 (colonies) . 7.5 . . DG18 (malt-extract agar) (potato dextrose agar) . (malt-extract agar) (potato dextrose agar) . . (Aspergillus versicolor Chaetomium spp. ) / . (Phialophora, Trichoderma, Acremonium, Chaetomium, Penicillium, Fusarium ) . . . , . . . . 7 15 . , (col

15、ony) . . 7.6 7.6.1 KS I ISO 16000 17:2012 9 DG18 (colony) . DG18 (Chaetomium, Stachybotrys ) (malt-extract agar) (potato dextrose agar) . DG18 (malt-extract agar) (potato dextrose agar) / (colony) . . “ ” . (7.5 ). . (colonies) / . 2 . . . 7.6.2 (impaction) 4 ( ). (cfu) CI (1) . IcfuIVnC = (1) ncfu：

16、 (cfu) VI： (m3) 100 L 200 L . . 15 100 L(0.1 m3) 23 29 200 L(0.2 m3) 35 ncfu 15 23 29 35 102 VI 0.1 0.1 0.2 0.2 0.6 2I107.11706.0102=C KS I ISO 16000 17:2012 10 (colonies) , . . . 80 % . nj ncfu,c . ncfu,c ncfu1.075/(1.052 f)0.438 f 0.95 ncfu： (cfu) f ： ncfu/nj . i) 20 40 (colonies), 100 (colonies). ii) (colonies) 100 / (colonies) . iii) , (colony) (colony) . 7.6.3 (colonies) . 1： 10 . (colonies) (7.4 ). , KS I ISO 8199 . CS(cfu/mL) (2) . ScfuSVnC = (2) ncfu： (colonies) VS： (mL). (3) . VS npVtfd(3) np： Vt：