KS I ISO 16221-2006 Water quality－Guidance for determination of biodegradability in the marine environment《水质 海洋环境生物降解性测定指南》.pdf

1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 9 29 http:/www.kats.go.krKS I ISO 16221 KS I ISO 16221： 2006 (2011 )I ISO 16221： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2001 12 18 ：2006 9 29 ：2011 12 26 2011-0623 ： ： ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.060.70 KS I

2、 ISO 16221： 2006(2011 ) Water quality Guidance for determination of biodegradability in the marine environment 2001 1 ISO 16221 Water quality Guidance for determination of biodegradability in the marine environment . . 1. (static) . . DOC (KS M ISO 7827), (the closed bottle test)(KS M ISO 10707), (K

3、S M ISO 10708), (KS M ISO 9439) (ISO 14593) . . a) . b) , (ISO 10634 ). c) . d) . . . ISO 14593 KS M ISO 15462 , ISO 14592 . 2. . . . IEC ISO . KS M ISO 7827 “ ” KS M ISO 9439 ( ) KS M ISO 10707 ( ： ) KS M ISO 10708 2 I ISO 16221： 2006 2 ISO 14592 1 Water quality Evaluation of the aerobic biodegrada

4、bility of organic compounds at low concentrationsPart 1： Shake-flake batch test with surface water or surface water/sediment suspensions ISO 14592 2 Water quality Evaluation of the aerobic biodegradability of organic compounds at low concentrationsPart 2： Continuous flow river model with attached bi

5、omass ISO 14593 Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium Method by analysis of inorganic carbon in sealed vessels(Co2headspace test) 3. . 3.1 (ultimate aerobic biodegradation) ( ) 3.2 (primary biodegradation) ( ) 3.3 (total organic carbon)

6、TOC 3.4 (dissolved organic carbon) DOC 15 40 000 ms 2 0.2 m 0.45 m . 3.5 (total inorganic carbon) TIC 3.6 (dissolved inorganic carbon) DIC 15 40 000 ms 2 0.2 m 0.45 m . 3.7 (chemical oxygen demand) COD ( ) . 3.8 (biochemical oxygen demand) BOD ( ) . 3.9 (theoretical oxygen demand) I ISO 16221： 2006

7、3 ThOD ( ) . 3.10 (theoretical amount of formed carbon dioxi de) ThCO2 ( ) . 3.11 (theoretical amount of inorganic carbon) ThIC ( ) . 3.12 (lag phase) , 10 % . 3.13 (maximum level of biodegradation) . 3.14 (biodegradation phase) 90 % . 3.15 (plateau phase) . 3.16 (pre-exposure) 3.17 (preconditioning

8、) 4. . , . , . . . DOC( ) DOC (KS M ISO 7827) . BOD( ) (KS M ISO 10707) (KS M ISO 10708) (ThOD) (COD) . CO2 (KS M ISO 9439) (ThCO2) . TIC( ) CO2 (ISO 14593) (ThIC) . I ISO 16221： 2006 4 ( 14C) (ISO 14592) . 5. , 1 15 25 . . 6. (6.2) (6.3) . . 6.1 1 mg DOC 6.2 6.2.1 . . 10 30 . . ( , ) DOC( KS M ISO

9、8245 ) . , , . 105cells/mL . (6.3) . . 1. . , ( , x %), . ( , ) . 2. , , . (blank) DOC BOD . 1 . DOC ( 10 % ) (6.3) . (TIC) . ISO 14593 . pH . (HCI) 6.5 pH 1 . I ISO 16221： 2006 5 (NaOH) pH . , . (6.2.2). 6.2.2 1 000 mL 800 mL (6.2) 10 mL a) 1 mL b) 1 000 mL(6.2) . 6.2.2.1 a) anhydrous potassium dih

10、ydrogenphosphate(KH2PO4) 8.5 g anhydrous dipotassium hydrogenphosphate(K2HPO4) 21.75 g disodium hydrogenphosphate dihydrate(Na2HPO4 2H2O) 33.4 g (NH4Cl) 0.5 g (6.1) 1 000 mL 6.2.2.2 b) 1 000 mL , 0.25 g (III) hexahydrate(FeCl3 6H2O) (6.1) . (HCl) . 6.3 . 1 000 mL 800 mL g) 1 mL h) j) g) 1 000 mL . .

11、 , , , . (6.2.1 ) . . DOC 6.2.1 . 6.3.1 a) (NaCl) 47.8 g (Na2SO4) 8.0 g (KCl) 1.4 g (NaHCO3) 0.04 g (KBr) 0.2 g (H3BO3) 0.06 g (NaF) 0.006 g (6.1) 1 000 mL 6.3.2 b) 1 000 mL 203.3 g magnesium sulfate heptahydrate(MgSO4 7H2O) (6.1) . 6.3.3 c) 1 000 mL 147 g calcium chloride dihydrate(CaCl2 2H2O) (6.1

12、) . 6.3.4 d) 1 000 mL 26.61 g strontium chloride hexahydrate(SrCl2 6H2O) I ISO 16221： 2006 6 (6.1) . 6.3.5 e) 1 000 mL 136.1 g anhydrous dipotassium hydrogenphosphate(K2HPO4) (6.1) . 6.3.6 f) 1 000 mL 26.74 g (NH4Cl) (6.1) . 6.3.7 g) g) 500 mL a), 53.3 mL b), 10.3 mL c), 0.9 mL d), 1.25 mL e) 1 mL f

13、) (6.1) 1 000 mL . g) 35 % . 6.3.8 h) 100 mL 15 mg . 6.3.9 i) manganese sulfate monohydrate(MnSO4 H2O) 60.46 mg zinc sulfate heptahydrate(ZnSO4 7H2O) 85.6 mg ammonium molybdate tetrahydarte(NH4)6Mo7O24 4H2O) 73.7 mg (6.1) 1 000 mL 6.3.10 j) 1 000 mL 89 mg iron(III) chloride hexahydrate(FeCl3 6H2O) .

14、 (HCl) . 6.4 6.4.1 (6.1) , . ( 2 ) . . KS M ISO 10634 . 6.4.2 BOD 60 % , DOC 70 % , 80 % (aniline) (sodium benzoate) . (6.1) . 6.4.3 ( , ) (6.1) (6.4.1) (6.4.2) . 7. . . . 8. . (6.2 6.3) (6.4.1) ( FT) (6.2 6.3) ( FB) (6.4.2) (6.2 6.3) ( FC) I ISO 16221： 2006 7 (6.4.3) (6.2 6.3) ( F1) (6.4.1) (6.2 6.

15、3) ( FS) . . , 10 g/L (II)(HgCl2) 1 mL . 2 . 1. . . 2. , (KS M ISO 10707 ISO 14593) . . ( 2 ) (6.2 6.3), (6.4.1) (6.4.2) 1 . 1 FT FT FBblank FBblank FC Fl ( ) Fs ( ) 2 , KS M ISO 7827 540(DOC) DOC 60 KS M ISO 10707 210( ) BOD 60 KS M ISO 10708 100 (ThOD) BOD 60 KS M ISO 9439 20 (TOC) CO260 ISO 14593

16、 2040(TOC) TIC 60 DOC , (管 ) . pH 7. 8. . pH . . (5. ) . . ( 2 ) . ( 0) ( t) . ( t) FT FS I ISO 16221： 2006 8 . (ISO 14592 ). ( ) , . . 28 . 60 . . , BOD (nitrate) (nitrite) . 9. ( ) . . . FT(20 % .) . . , ( ) , . , “ ” . DOC , . ( ), , , DOC . FC FS FI . . FI 25 % FI , . . FS (10 % ) DOC, CO2 BOD .

17、 10. FC 14 60 % (DOC 70 %) . 11. DOC OSPARCOM . 1 ( ). I ISO 16221： 2006 9 3 OSPARCOM 60 % 28 % 60 % 60 % 50 % (Sodium benzoate) A:100 B:100 C:91 D:100 A:100 B:100 C:100 D:100 A:1.6 B:0.9 C:2.1 D:2.4 A:2.1 B:2.9 C:4.6 D:2.6 Anco Green B A:60 B:92 C:75 D:71 A:80 B:100 C:83 D:85 A:2.4 B:1.3 C:3.0 D:2.1 A:3.9 B:2.5 C:5.9 D:6.1 Aquamul BII A:0 B:0 C： 0 D:0 A:0