KS I ISO 16266-2007 Water quality－Detection and enumeration of Pseudomonas aeruginosa－Method by membrane filtration《水质 去硝假单胞菌的检测及计数 膜滤法》.pdf

1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 16266 KS I ISO 16266 ：2007 (2012 ) 2007 12 7 http:/www.kats.go.krKS I ISO 16266：2007 : ( ) ( ) ( ) () ( ) () : (http:/www.standard.go.kr) ： ：2007 12 7 ：2012 12 27 20120787 ： ： (ISO/TC147) ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO 162

2、66：2007 .1 1 1 2 1 3 .1 4 2 4.1 .2 4.2 .2 4.3 .2 5 , 2 5.1 2 5.2 .3 6 5 6.1 .5 6.2 .5 6.3 5 6.4 .5 7 5 8 5 8.1 .5 8.2 .6 8.3 .6 8.4 6 8.5 .6 9 .7 10 .7 11 .8 12 8 13 .8 A() 9 B() .10 11 i KS I ISO 16266：2007 (2012 ) Water qualityDetection and enumeration of Pseudomonas aeruginosaMethod by membrane f

3、iltration 2006 1 ISO 16266, Water qualityDetection and enumeration of Pseudomonas aeruginosaMethod by membrane filtration , . . . . . 1 . , (background flora) . 2 . . ( ) . KS H ISO 68871, , 10 1： 10 KS M ISO 3696, KS M ISO 56671, 1： KS M ISO 56672 1) , 2： KS M ISO 56673, 3： KS M ISO 7704, KS M ISO

4、8199, KS M ISO 19458, 3 1) KS M ISO 56671 KS M ISO 56672 KS M ISO 56671 . KS I ISO 16266：2007 . 3.1 (Pseudomonas aeruginosa) (cetrimide) (pyocyaninn) (36020) nm , (Acetamide) 4 4.1 0.45 m (membrane filter) . . 4.2 . . 4.3 2 ( B ). , , . . 5 , . . KS M ISO 3696 3 . 5.1 . 5.1.1 (Pseudomonas agar) /CN

5、5.1.1.1 16.0 g (Casein hydrolysate) 10.0 g () 10.0 g () 1.4 g 2 KS I ISO 16266：2007 10 mL (Agar) 11.0 g18.0 g 1 000 mL . . CN ()(Hexadecyltrimethyl ammonium bromide)(cetrimide) 0.2 g (Nalidixic acid) 0.015 g 5.1.1.2 ( ) 1 000 mL , , , , . 10 mL . , (1213) 15 . 4550 . CN 2 mL , . 5 mm . pH 25 7.10.2

6、. (53) 1 . 4 . . 5.2 5.2.1 Kings B 5.2.1.1 20.0 g 10 mL 2(K 2 HPO 4 ) 1.5 g 7 1.5 g (Agar) 15.0 g 1 000 mL 5.2.1.2 . 4550 , pH 25 7.20.2 . (1213) 15 5 mL . . (53) 3 . 5.2.2 (aetamide broth) 5.2.2.1 A 2(KH 2 PO 4 ) 1.0 g () 0.2 g 3 KS I ISO 16266：2007 2.0 g 0.2 g 900 mL pH 25 7.00.5 . . , . B (Na 2 M

7、oO 4 2H 2 O) 0.5 g 7 (FeSO 4 7H 2 O) 0.05 g 100 mL 5.2.2.2 , A, 900 mL B, 1 mL (5.2.2.1). 1 L . (1213) 15 5 mL . (53) 3 . 5.2.3 5.2.3.1 5.0 g (Meat extract) 1.0 g (Yeast extract) 2.0 g 5.0 g (Agar) 15.0 g 1 000 mL 5.2.3.2 . (1213) 15 . pH 25 7.40.2 . . (53) 1 . 5.2.4 5.2.4.1 -p- 2 (tetramethyl-p-phe

8、nylenediamine dihydrochloride) 0.1 g 10 mL 5.2.4.2 4 KS I ISO 16266：2007 -p- 2 . . . . 5.2.5 (nessler) 5.2.5.1 (HgCI 2 ) 10 g 7 g 16 g ( ) 100 mL 10 g 7 g , 16 g 50 mL . 100 mL . (borosilicate) 1 . . . 6 . 6.1 (1705) 1 (1213) 15 . 6.2 (362) . 6.3 (36020) nm . 6.4 (nominal pore size) 0.45 m . KS M IS

9、O 7704 . 7 KS M ISO 56671, KS M ISO 56672, KS M ISO 56673 KS M ISO 19458 . 8 8.1 KS M ISO 8199 , KS H ISO 68871 5 KS I ISO 16266：2007 . 8.2 0.45 m . KS M ISO 8199 , CN (5.1) . 8.3 (362) (444) h , . 8.4 (222) h (444) h . /() . . . (non-pyocyanin) . , , Kings B . (444) h (222) h . 9. . 1 . 1 CN CN Kin

10、gs B / NT aNT NT (/ ) NT NT NT NT NT aNT： . 8.5 8.5.1 2 (362) (222) h . 2 (8.5.2) . 6 KS I ISO 16266：2007 8.5.2 (5.2.4) 2 3 . (, ) , , . 10 . . 8.5.3 Kings B 8.5.1 Kings B 2 5 (362) . 2) . 5 . 8.5.4 8.5.1 2 , (362) (222) h . (nessler) ( 5.2.5) 1 2 , . 8.5.5 (/ ) , (8.4 8.5.3), (8.5.4) . ( 1 ). 9 . ,

11、 250 mL ( 1 2, 3 ). 100 mL. P / , . F R n F c F , n R Kings B c R Kings B , , PF(c F /n F )R(c R /n R ) . 10 2) 24 7 KS I ISO 16266：2007 . a) b) c) 9. d) , 11 5 6 ( 2 ). 2 (%) % 24 48 101.7 100.1 1 24 48 92.6 91.3 2 24 48 104.4 124.8 3 24 48 94.7 91.3 4 12 . 13 NCTC 10332 , (E. coli) NCTC 9001 . 8 K

12、S I ISO 16266：2007 A () . , . Hugh Leifson , . (98 %) . 42 4 4 42 (Pseudomonas fluorscens) . , , . () 90 % . 9 KS I ISO 16266：2007 B () . 10 KS I ISO 16266：2007 11 1 Council Directive 80/777/EEC on the approximation of the laws of the Member States relating to the exploitation and marketing of natur

13、al mineral waters. Official Journal of the European Communities, L229, 1980, pp. 110 2 Directive 96/70/EC of the European Parliament and of the Council amending Council Directive 80/777/EEC on the approximation of the laws of the Member States relating to the exploitation and marketing of natural mi

14、neral waters. Official Journal of the European Communities, L299, 1996, pp. 2628 3 Council Directive 98/83/EC on the quality of water intended for human consumption. Official Journal of the European Communities, L330, 1998, pp. 3253 153787 1 145 3(16) (02)26240114 (02)26240148 http:/ KS I ISO 16266 ：2007 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water quality Detection and enum ration of Pseudomonas aeruginosa e Method by membrane filtration ICS 13.060.70 Korean Agency for Technology and Standards http:/www.kats.go.kr