KS I ISO 20665-2011 Water quality－Determination of chronic toxicity to Ceriodaphnia dubia《水质 对网纹水蚤属Ceriodaphnia dubia)慢性毒性的测定》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 20665 (Ceriodaphnia dubia) KS I ISO 20665:2011 2011 9 2 http:/www.kats.go.krKS I ISO 20665:2011 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：2011 9 2 2011-0308 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO 20665:2011 i . ii 1 1 2 1 3

2、 .1 4 2 5 .3 6 , 3 7 5 8 6 9 7 9.1 .7 9.2 7 9.3 .7 9.4 .8 9.5 .8 9.6 9 10 .10 10.1 .10 10.2 .11 11 .11 12 11 13 .12 A( ) ELENDT M4 .13 A.1 13 A.2 M4 13 B( ) 15 C( ) LC OLIGO 16 C.1 .16 C.2 .17 D( ) YCT 18 D.1 .18 D.2 18 D.3 (Cerophyll)( ) .18 D.4 YCT 19 E( ) 20 21 KS I ISO 20665:2011 ii 2008 1 ISO 2

3、0665, Water quality Determination of chronic toxicity to Ceriodaphnia dubia , . . , . , (71) . . KS I ISO 20665:2011 (Ceriodaphnia dubia) Water quality Determination of chronic toxicity to Ceriodaphnia dubia . . . . 1 (71) (Ceriodaphnia dubia) (Cladocera), (Crustacea) . . a) b) , , c) d) . 2 . . ( )

4、 . KS I ISO 5667 16:2009, 16： KS I ISO 5814, KS I ISO 6059, EDTA KS I ISO 10523, pH ISO/TS 20281, Water quality Guidance on statistical interpretation of ecotoxicity data 3 KS I ISO 20665:2011 2 . 3.1 , 2 , ( , ) 3.2 (daphnid) 3.3 (3.4) 10 . 3.4 3.5 x % ECx (3.3) x % 3.6 , 24 1 . 3.7 (3.3) (3.8) 3.8

5、 (3.9) , 10 . 3.9 , 4 KS I ISO 20665:2011 3 24 (71) . 7 60 % 3 . . . , EC10, EC20 EC50 x % ECx . 5 (252) . 2 . 16 8 / ( ) . (7.2) , / (7.8) 100 lx600 lx . . . , . 6 , , . 6.1 , 3 . 24 8 . , 6 , 14 12 . (6.4.1 6.4.2) (6.3.2 6.3.3) . . 8 . 1) (ephippia) . . 6.2 , 10 S/cm2) 6.3 6.3.1 1) Deinze Microbio

6、test ephippia . ISO . 2) 1 mS/mKS I ISO 20665:2011 4 ELENDT M4(6.3.2) (6.3.3) . (11.) . , (I), (I), (II), (II), , , , pH , ( ) ( ) , . 6.3.2 ELENDT M4 A ELENDT M4 . (KS I ISO 10523 ) pH 8.00.3, (KS I ISO 6059 CaCO3 ) (25020) mg/L . pH . EDTA , 2 , . 6.3.3 B . (KS I ISO 10523 ) pH 7.4 7.8 , (KS I ISO

7、 6059 CaCO3 ) (9010) mg/L . 6.4 6.4.1 1： 2 , (Chlorella vulgaris) ( (Selenastrum capricornutum) (Raphidocelis subcapitata) ) (Pseudokirchneriella subcapitata)3) (NF T90-3761 2 ). (6.3.2) 5 g/L 4) , . . ( LC OLIGO, C) . 5106 . (43) 10 . (9.3) . a) (Chlorella vulgaris) 1 L 121063) Ambleside (Freshwate

8、r Biological Association) . ISO .4) Sera Micron . ISO .KS I ISO 20665:2011 5 b) (Pseudokirchneriella subcapitata) 1 L 6106c) 1 L 500 L , (9.3) 10 % . 5) , . , , (6.3.2 6.3.3) . 2 . . 6.4.2 2： / (Cerophyll)6) / (EPA) 1002.0( 11, p.141) ( 5) ( 3 4 ). / (Cerophyll)/ (YCT) . . YCT D . YCT/ , . 1 L 7 mL

9、1 L YCT 7 mL . 15 mL 0.1 mL 15 mL YCT 0.1 mL . YCT . YCT , (43) . 2 YCT . (43) 10 . 6.5 (C6Cl5ONa), 5 (CuSO45H2O), (NaCl) (ZnSO4) . , (MSDS) . 7 5) Deinze Microbiotest . ISO .6) Cereal Grass Media-Cerophyll . ISO .KS I ISO 20665:2011 6 . 7.1 , 7.2 , . , / 1： 1 . , (6.3.2 6.3.3) (6.2) . 7.3 , . , ( ,

10、 , ) . 7.4 , 7.5 , 8 , 7.6 , 7.7 , 0.45 m, 0.22 m 7.8 7), (7.2) / 100 lx 600 lx 7.9 , KS I ISO 5667 16:2009 3.2 7.10 , 100 m 8 , KS I ISO 5667 16 . (7.9) . , 12 . , 0 4 24 . 72 , 2 18 . , , , 2 ( ) , . ( , , , ), 0.45 m (7.7) . 7) Grolux . ISO .KS I ISO 20665:2011 7 . . (43) . pH(KS I ISO 10523 ) (K

11、S I ISO 5814 ) (13.) . pH , pH 8.00.3 . , pH . (13.) pH . 9 9.1 (6.3.2 6.3.3) . , . , KS I ISO 5667 16 . 9.2 (8. 9.1) 5 , 3.2 . . ECx , x % x % . 10 . (3.8) . (3.3) . 10 . , . . , , 100 % . , , ECx . 9.3 (8. 9.1) (6.3.2 6.3.3) (6.4.1 6.4.2) . / (6.4.1) , KS I ISO 20665:2011 8 10 1 ( , 500 mL 50 mL).

12、 YCT/ (6.4.2) , 1.5 % ( , 15 mL YCT 0.1 mL 0.1 mL ). , (152) mL (502) mL . (152) mL (502) mL . (6.4.1 6.4.2) (6.3.2 6.3.3) . (152) mL (502) mL . 9.4 (252) (7.1) . , (7.2) 24 (6.1) . (7.4) . 9.5 / (6.4.1) , 1 (9.3) . 1 ( /2 ) 0 1a2 3b4 5 6 7c8 / / / a , . , . b , . , . c 8 , . , 9.3( ) . , . , 2 . YC

13、T/ (6.4.2) , (9.3) 2 . KS I ISO 20665:2011 9 2 (YCT/ ) 0 1 2 3 4 5 6 7a8 / a 8 , . 1 6 , 9.3( ) . , (species) . 9.6 . , (pH, , ) . ( E ). ( 1 2 ) , 1 , pH . , , pH(KS I ISO 10523 ) (KS I ISO 5814 ) . pH pH pH 0.3 . (252) 100 % . . . , 2 . . , . , . 100 m(7.10) . (7.5) . (7.6) . 60 % 3 . 7 6 8 . . , (6.3.2 6.3.3) . 2 5 % (glycerol) KS I ISO 20665:2011 10 . . (caudal spine) . 10 10.1 , Ne . Nt . Ne eN . tN . I (1) . 100tet=NNNI