KS I ISO 23611-1-2007 Soil quality－Sampling of soil invertebrates－Part 1：Hand－sorting and formalin extraction of earthworms《土质 土壤无脊椎动物的取样 第1部分 蚯蚓的手工拣取和福尔马林萃取》.pdf

1、 KS I ISO 236111 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 1： KS I ISO 236111 : 2007 (2012 ) 2007 11 7 http:/www.kats.go.krKS I ISO 236111：2007 : ( ) ( ) ( ) () ( ) E&M / : (http:/www.standard.go.kr) ： ：2007 11 7 ：2012 12 27 20120781 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO 23611

2、1：2007 ii 1 1 2 .1 3 .2 4 .2 4.1 (formalin).2 4.2 (formalin).2 4.3 (ethanol) .3 5 .3 5.1 (plastic vessels).3 5.2 (plastic gloves).3 5.3 (forceps).3 5.4 (piece of thick plastic sheeting) 3 5.5 (spade shovel).3 5.6 (dissecting microscope) 3 5.7 (balance)3 5.8 (water-can).3 5.9 (watering can).3 5.10 ,

3、, , 3 5.11 (thermometer) 3 5.12 (drying cabinet) 3 6 3 6.1 3 6.2 (preservation).6 6.3 (determination of biomass)6 7 .6 8 7 A() .8 A.1 (electrical extraction method) 8 A.2 (extraction with mustard)8 B() .9 C() TSBF .10 11 i KS I ISO 236111：2007 ii . . A() B() C() TSBF KS I ISO 236111 : 2007 (2012 ) 1

4、： Soil qua Part 1 ：Hand sorting and formalin extraction of earthworms lity Sampling of soil invertebrates 2006 1 ISO 236111, Soil qualitySampling of soil invertebratesPart 1： Handsorting and formalin extraction of earthworms , . 1 ( ) . , . . KS M ISO 103811( 38 ) , KS M ISO 112683 . / ( C ). (semit

5、errestrial soils) , ( ) . . KS M ISO 10390, KS M ISO 10694, KS M ISO 11272, KS M ISO 11274, KS M ISO 11277, KS M ISO 11461 KS M ISO 11465 pH , C/N, . 2 KS M ISO 11074( ) . 2.1 (earthworm) megadrile ( ： cm 1 m ) (Oligochaeta), (Annelida) KS I ISO 236111：2007 Lumbricidae(Holarctic), Glossoscolecidae()

6、, Eudrilidae() Megascolecidae , ( ) (species) 2.2 (peregrine species) 1 Aporrectodea caliginosa( ) pan-tropical Pontoscolex corethrurus( ) 2 15 2.3 (clitellum) (ring) (saddle) , 3 . ( 0.25 m 2 ) ( 50 cm50 cm20 cm) 5,20 . . ( ). . ( 1 m 2 ) . 1 ( ) ( A ). 11,33 . 2 ( ) . 1 . 3 ( ) B (Lumbricidae) . 4

7、 4.1 (formalin) 4 % () 4.2 (formalin) 37 % () 2 KS I ISO 236111：2007 4.3 (ethanol) 70 %() 5 . 5.1 (plastic vessels) , 250 mL 500 mL 5.2 (plastic gloves) 5.3 (forceps) 5.4 (piece of thick plastic sheeting) 1 m 2 2 m 25.5 (spade shovel) 5.6 (dissecting microscope) (1040) 5.7 (balance) 0.01200 g . 5.8

8、(water-can) 20 L( 20 L) 5.9 (watering can) 5.10 , , , 5.11 (thermometer) 5.12 (drying cabinet) 6 6.1 6.1.1 . . ( 7, 8, 15). (： ) ( 3 KS I ISO 236111：2007 .) . 15 . . . . . ( , , ) 22 . , TSBF ( C ). 30 . B . , . . 6.1.2 (hand-sorting) . 1 20 cm (Holartic) 50 cm50 cm . ( pH 4.5 ) (1 m 2 ) (KS M ISO 1

9、12683). ( ) 1/8 m 2 24 . (1/16 m 2 ： 36 ) (16 ) . (5.5) 20 cm (20 cm ). (5.4) . . . (5.2) (5.3) . . . 1 (clitellum) (). . 70 % (4.3) 250 mL 500 mL (5.1) 1 30 . 24 . ( ) (5.10) . 4 % ()(4.1) ( ) . 2 (prostomium) ( 1 ) 4 KS I ISO 236111：2007 . . 6.1.3 (formalin extraction) . (5.8) ( 20 L). ( 0.5 %) (5

10、.9) 5 L 37 % (4.2) () 25 mL . 5 L 37 % () 25 mL 0.2 % . . 20 L . . . 30 . (5.2) (5.3) . . ( ) . . 70 % (4.3) 250 mL 500 mL (5.1) 1 30 . 24 . ( ) (5.10) . (, , ) 4 m 2 . . . ( Lumbricus terrestris Aporrectodea longa) ( ) . ( 0.5 cm) (cast) . . 1 17 . . 7 . 2 , . ( ). ( ) . 37 % “ (Material Safety Dat

11、a Sheet)” (： , ： ). . 5 KS I ISO 236111：2007 6.2 (preservation) . a) 4 % ()(4.1) 4 . 1 2 . 70 % ()(4.3) . b) 70 % ()(4.3) 4 % ()(4.1) 98 % 2 % . . . 37 . 6.3 (determination of biomass) . 5 (5.7) . 70 % ()(4.3) / . . ( 7, 15), 10 % 20 % . . . 0.15 19 . 19 ( .) . ( ). 7 ( ). (abundance, ) ( ) (dominan

12、ce ratio, ) ( / ) ( ). ( ) . 0.25 m 2 6 KS I ISO 236111：2007 4 ( 50 cm50 cm ). ( .) (5.6) . 8 . a) b) c) ( ) d) e) , , f) , ( , ) g) h) i) 1 m 2 j) k) 7 KS I ISO 236111：2007 A () A.1 (electrical extraction method) Thielemann 30 8 ( 52 cm) 8 (octet method) . . . ( ) . ( 33) . , . A.2 (extraction with

13、 mustard) Gunn 11 . . 60 g 10 L . ( 14) ( 33 ). (allyl isothiocyanate) 35 . . ( Lumbricus terrestris) 18 . 8 KS I ISO 236111：2007 B () (lumbricid) 4, 10 27 . 29 . 16 . . Glossoscolecidae( ), Eudrilidae() Megascolecidae, () . , 101 (36 megascolecids, 34 lumbricids 31). 3 . . . 9 KS I ISO 236111：2007

14、C () TSBF “ TSBF”( ： 1 ) . . . TSBF . 3 . a) . . b) 25 cm25 cm15 cm (monoliths) . c) (0.2 %, ) 10 . 6 ( ) 3 . 8 10 7 m . . 10 KS I ISO 236111：2007 1 ANDERSON, J.M., INGRAM, J.S.I., 1993, Tropical Soil Biology and Fertility：A Handbook of Methods. C A B International, Wallingford, UK 2 BBODSCHG, 1998,

15、 Gesetz zum Schutz vor schdlichen Bodenvernderungen und zur Sanierung von Altlasten(Bundes-BodenschutzgesetzBBodSchG) vom 17. Mrz 1998. BGBL I, Nr. 16, S. pp. 502510 3 BLAKEMORE, R., 2002, Cosmopolitan Earthwormsan Eco-Taxonomic Guide to the Peregrine Species of the World. VermEcology, P.O. Box 414

16、Kippax, ACT 2615, Australia. 426 pages and 80 figures 4 BOUCH, M., 1972, Lombriciens de France. Ecologie et Systmatique. Paris, France：INRA Publ. 72-2, Institut National de Recherches Agriculturelles. 671 pages 5 BRETSCHER, K., 1896, Die Oligochaeten von Zrich. Revue Suisse Zoologie, 3, pp. 499532 6