KS I ISO 9998-2008 Water quality－Practices for evaluating and controlling microbiological colony count media used in water quality tests《水质 水质试验中使用的微生物菌落计数培养基的评定和控制技术》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 9998 KS I ISO 9998:2008 2008 11 24 http:/www.kats.go.krKS I ISO 9998:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 1998 4 30 ： 2008 11 24 2008-0794 ： ： ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 9998:2008 Water quality Practices fo

2、r evaluating and controlling microbiological colony count media used in water quality tests 1991 1 ISO 9998, Water quality Practices for evaluating and controlling microbiological colony count media used in water quality tests , . 1 . . . . 2 . . ( ) . KS I ISO 8199, 3 / 3.1 , . a) b) c) . . , KS I

3、ISO 9998:2008 2 . 3.2 A . 4 4.1 . 4.2 pH pH pH . pH 25 0.2 . pH , pH . pH , . , . pH pH . , 4.3 . 6. . . pH ( , .) . (membranes) . KS I ISO 9998:2008 3 4.4 (media) , . , . ( ) . 2 3 1) . ( ) . pH . 4.5 . . , pH ( ) . , 6 12 . C 1 B . 4.6 . . . . ( ), , , . . . 30 43 45 . . . 4.7 , , . , 2 (KH2PO4) .

4、 1) 4 Milli Q . KS I ISO 9998:2008 4 . , , . 4.8 . . , , , , , , , , , , . . . . 4.9 (shelf life) , . ( ) . . . ( ) . 5 5.1 . , . C 2 , . , . 5.2 , ( , , ) , , , . KS I ISO 9998:2008 5 , . a) . b) . c) . . a) 1 mL b) 5.3 , . . . . 5.4 , . 100 mm . . 50 mm 60 mm . 5.5 5.5.1 , , , . . 2 4 . 5.5.2 . .

5、. 24 2 4 (stress) . . , . 2 4 . , . . 6 KS I ISO 9998:2008 6 6.1 (spread plate procedures) 0.5 mL . , . 30 35 15 18 . 6.2 , (stress) 50 200 0.5 mL 0.5 mL . , . . (spreader) . . . 6.3 , 400 500 0.5 mL ( A) 4 000 5 000 0.5 mL ( B) . 0.5 mL 50 200 , . 0.5 mL A B , 50 200 . . . 6.2 . 6.4 , (6.2 6.3). 1.

6、0 mL . 6.5 1 mL 30 mL 50 200 KS I ISO 9998:2008 7 , . 0.5 mL (25100 ) . . . . 6.6 1 mL 400 500 ( A), 4 000 5 000 ( B) . (A B), 1 mL 50200 . 1 mL 30 mL . . , mL 50 200 . . . 6.5 . 6.7 0.5 mL . mL 50 200 . . 6.2 . 6.8 (6.4 6.7 ). 6.9 50 200 , 30 mL . 10 mL . . 0.5 mL 50200 . . KS I ISO 9998:2008 8 6.5

7、 . 7 7.1 (10) , . 7.2 A . . 7.3 (0 1 0.5 ). 7.4 . 7.5 (4 ), . . 7.6 , (variance) , A . 2 ( A ). 7.7 F 1 . 7.8 , t . 7.9 Posteriori Student Newman Keuls . KS I ISO 9998:2008 9 A ( ) A.1 m . , , , . m “ ” . . A.2 , . k m . m mk (ANOVA) . A.4.1 A.4.2 . m k . . (ANOVA) (A.4.3 ). k 2 . . . A.3 , . . . .

8、s k m ANOVA . (SM) . , . A.5 . k . . , . . . . A.4 A.4.1 1： k m KS I ISO 9998:2008 10 A.4.1.1 Cij mk( A.1) . A.1 1 2 3 m 1 C11C12C13 C1m2 C21C22C23 C2m k Ck1Ck2Ck3 CkmA.4.1.2 A.1 ( A.2 ). Xij log Cij . A.2 1 2 3 m 1 X11X12X13 X1m2 X21X22X23 X2m k Xk1Xk2Xk3 Xkm X 1X 2X 3 X mX A.4.1.3 (ANOVA) ( A.3).

9、A.3 DF SS MS F m 1 A )1( mACkmmA )1()1( m(k 1) C )1( kmC mk 1 B , A . CTXXX22221kAm k ： m ： X 1 ： A.2 1 X 2 ： A.2 2 X m： A.2 m X ： A.2 KS I ISO 9998:2008 11 CT ：mk2)X( . B211X 221X 2Xkm CT C . C B A . A.4 . A.4 1 2 3 1 2 3 4 5 58 45 56 53 44 50 47 48 52 44 40 47 54 42 46 ( )( A.5 ). A.5 1 2 3 1 2 3

10、4 5 4.060 3.807 4.025 3.970 3.784 3.912 3.850 3.871 3.951 3.784 3.689 3.850 3.989 3.738 3.829 19.646 19.368 19.095 58.109 A.5 X 1 19.646, X 2 19.368, X 3 19.095, X 58.109 CT . CT 4110.22515109.58)X(22mk A . KS I ISO 9998:2008 12 5XXX232221 A CT 225.140 8 225.110 4 0.030 4 2015.024030.014030.01 mmA ,

11、 B (4.060)2 (3.807)2 (3.829)2 CT 225.274 2 225.110 4 0.163 8 , C . C B A 0.163 8 0.030 4 0.133 4 124133.0)1(kmC 0.011 1 F2,12 1011.02015.0 1.37 A.3 A.6 . A.6 DF SS MS F 2 12 0.030 4 0.133 4 0.015 2 0.011 1 1.37 14 0.163 8 F 2 12 5 % F 3.88 , F 1.37 . 5 % . A.4.2 F , . . ( ) . F . . A.4.2.1 . 5 . A.7 (Cij) .