KS I ISO TR 11044-2011 Water quality－Scientific and technical aspects of batch algae growth inhibition tests《水质 批量藻类生长抑制试验的科学和技术问题》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO TR 11044 , KS I ISO TR 11044:2011 2011 9 2 http:/www.kats.go.krKS I ISO TR 11044:2011 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：2011 9 2 2011-0308 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO TR 11044:2011 i . ii 1 1 2 1 3 .1 4 I

2、SO .2 5 4 5.1 .4 5.2 (Pseudokirchneriella subcapitata) 5 5.3 (Desmodesmus subspicatus) 8 5.4 (Skeletonema costatum).9 5.5 (Phaeodactylum tricornutum) .12 6 .14 6.1 14 6.2 pH 16 6.3 18 6.4 21 6.5 23 26 KS I ISO TR 11044:2011 ii 2008 1 ISO/TR 11044, Water quality Scientific and technical aspects of ba

3、tch algae growth inhibition tests , . , , , ( 9) . , . . (Skulberg) ( 50) (Selenastrum capricornutum Printz) . ( 55). 1959 . , . , (Raphidocelis subcapitata) ( ) Pseudokirchneriella subcapitata (Korshikov) Hindak ( 20). . 43 . , ( 26) . ( 28) . , . . . . ( 57) “- ” . OECD ISO 1970 . OECD , ISO . , .

4、 1978 ISO . 1980 1982 , ISO 8692:1989 ISO 8692:2004 . 1982 1991 ISO/DIS . ISO 10253:1995 ISO 10253:2006 . , , , ISO 14442:1999 ISO 14442:2006 . . KS I ISO TR 11044:2011 , Water quality Scientific and technical aspects of batch algae growth inhibition tests 1 KS I ISO 8692 , KS I ISO 10253 , , . (Nor

5、wegian Institute for Water Research： NIVA) . 2 . . ( ) . KS I ISO 8692, KS I ISO 10253, KS I ISO 14442, , , 3 . 3.1 (effective concentration) ECx x % KS I ISO 8692 , ECx ErCx , “r” “ (rate)” . , EbCx , “b” “ ” ( 6.5 ). KS I ISO TR 11044:2011 2 3.2 (specific growth rate) dtdnn1= n ： ( /mL) t ： (days)

6、 1 . 2 KS I ISO 8692 4 ISO KS I ISO 8692 KS I ISO 10253 , . , , 72 . 3 5 , 6 . , , . . 72 24 . (Pseudokirchneriella subcapitata) 1 . . , . 72 . 2 / . - (REGTOX)1) . 10 % 50 % ( EC10 EC50) . 1) http:/eric.vindimian.9online.fr/ (2008-11-14)KS I ISO TR 11044:2011 3 1 (K2Cr2O7) = 0( ) 5 (K2Cr2O7) = 1 mg

7、/L 2 (K2Cr2O7) = 0.25 mg/L 6 (K2Cr2O7) = 1.6 mg/L 3 (K2Cr2O7) = 0.4 mg/L n , 103 cells/mL 4 (K2Cr2O7) = 0.63 mg/L t , h 1 K2Cr2O7 (P. subcapitata) ( ) EC1010 % EC5050 % (K2Cr2O7) , mg/L 2 K2Cr2O7 / KS I ISO TR 11044:2011 4 5 5.1 (phyla) , . . (interspecies) 3 4 (1 000 10 000 ) ( 2, 24, 54) . , ( ) (

8、 8, 21, 33, 53). KS I ISO 8692 (P. subcapitata) (Scenedesmus subspicatus) (Desmodesmus subspicatus) . KS I ISO 10253 2 , (Skeletonema costatum) (Phaeodactylum tricornutum) . EPA ECOTOX ISO OECD 5 000 42 % ( 3 ) . , . 1 5 (Navicula pelliculosa) 2 6 3 7 4 - ne (Anabaena flos-aquae) a b 3 EPA ECOTOX KS

9、 I ISO TR 11044:2011 5 KS I ISO 8692 KS I ISO 10253 1 . KS I ISO 8692( ) KS I ISO 10253( ) . 21 80 mol/m2 s2) . 100 m (orifice tube) (Coulter Multisizer M3)3) . 104 . , . “ ” . , 1 . 1 ISO ( ) m3 mg NIVA/CHL 1 CCAP 278/4 72 3.0 108 NIVA/CH 55 SAG.86.81 139 5.3 108 NIVA/BAC 1 115 4.6 108 NIVA/BAC 2 5

10、6 1.9 1085.2 (Pseudokirchneriella subcapitata) , ISO OECD . 1959 ( CCAP 278/4, ATCC 22662, 61.81 SAG, UTEX 1648 ) ( 50). . 4 . 2) KS I ISO 8692 KS I ISO 10253 “ (illuminance)” “ (light intensity)” . (PAR) 400 nm 700 nm . KS I ISO 8692 KS I ISO 10253 , 6 000 Ix 10 000 Ix .3) . ISO .KS I ISO TR 11044:

11、2011 6 a) b) c) d) 4 KS I ISO 8692 KS I ISO 10253 (solitary) . 8 . , . 5 . , . , 2 . KS I ISO TR 11044:2011 7 d (spherical) , m d (spherical) , m nc Vc a) b) 5 KS I ISO 8692 KS I ISO 8692 1.4 d1 . 72 67 . KS I ISO 8692 . / 6 . KS I ISO TR 11044:2011 8 1 14.5 2 19.6 3 22.7 4 25.6 5 29.1 E , mol/m2 s

12、, d-1 KS I ISO 8692 . a) 1 30 mol/m2 s 2 50 mol/m2 s 3 70 mol/m2 s 4 80 mol/m2 s 5 90 mol/m2 s 6 100 mol/m2 s T , , d-1 KS I ISO 8692 . b) 6 KS I ISO 8692 , 5 , . 32 . KS I ISO 8692 (21 25) . 90 mol/m2 s , (17 27) (R2= 0.995 1) 1 0.16 d-1 . 21 1.38 d-1 25 2.02 d-1 . . 20 , (30100) mol/m2 s . , , 22.

13、7 , (60100) mol/m2 s 0.2 d-1 . 90 mol/m2 s . 20.8 50 mol/m2 s 1.31 d-1 100 mol/m2 s 1.51 d-1 35 . 5.3 (Desmodesmus subspicatus) . KS I ISO 8692(SAG 86.81 CCAP 276/22 UTEX 2594) 1953 KS I ISO TR 11044:2011 9 (Brinkmann) . 17 , . , 4 8 4 b) . 17 (3.5 5.0) m (4.0 6.0) m . KS I ISO 8692 7 . d (spherical

14、) , m nc a) d (spherical) , m Vc b) 7 KS I ISO 8692 (4 7) m . , 15 m . 5.4 (Skeletonema costatum) . KS I ISO 10253 2 . CCAP 1077/1C( : , 1970 ) NIVA BAC 1( : Fjord, 1962 ). CCAP , CCAP 1077/1C , 2 (CCAP 1077/3 1077/5) . . “ (species complex)” “ ” (S. grethae) ( CCAP 1077/3 1077/4) (S. pseudocostatum

15、) (CCAP 1077/7) ( 49 ) . CCAP 1077/1C . NIVA BAC 1 . KS I ISO 10253 NIVA BAC 1 8 . 4 c) , 6 m . KS I ISO TR 11044:2011 10 d (spherical) , m nc a) d (spherical) , m Vc b) 8 KS I ISO 10253 NIVA BAC 1( ) KS I ISO 10253 , ( 13). ISTPM P4, NIVA BAC 1, CCAP 1077/1C, CCAP 1077/3 CCAP 1077/5 . , 3,5- (DCP)

16、. 2 . 2 S. costatum EC50mg/L 3,5- EC50mg/L d-1 m3/cell ISTPM P4 6.6 1.4 2.643.36 563 NIVA BAC 1 2.6 0.95 1.682.11 120 CCAP 1077/1C 3.9 1.2 1.651.94 169 CCAP 1077/3 7.4 1.4 2.642.40 596 CCAP 1077/5 5.7 1.3 2.252.40 454 KS I ISO 10253 , “ (Skeletonema costatum) ” . . KS I ISO 10253 BAC 1 9 . 80 mol/m2

17、 s 70 ( ) . 13 21 . KS I ISO TR 11044:2011 11 (R2=0.996 2) 0.141 d-1 . 24 , 32.5 . . 75 mol/m2 s 21 2.0 d-1 . . T , , d-1 9 80 mol/m2 s KS I ISO 10253 S. costatum (NIVA BAC 1) (070 ) ( 2 ), 5 103 cells/mL 72 . 10 a) . , 48 3.5-DCP 1 mg/L 72 . ISO PARCOM ( 1, 15). , 48 . KS I ISO 10253:2008 , . KS I

18、ISO TR 11044:2011 12 1 (3.5-DCP) = 0( ) 2 (3.5-DCP) = 0.63 mg/L 3 (3.5-DCP) = 1 mg/L 4 (3.5-DCP) = 1.6 mg/L 5 (3.5-DCP) = 2.5 mg/L 6 (3.5-DCP) = 4 mg/L n , 103 cells/mL t , h a) (NIVA BAC 1) 1 (3.5-DCP) = 0( ) 2 (3.5-DCP) = 1 mg/L 3 (3.5-DCP) = 1.6 mg/L 4 (3.5-DCP) = 2.5 mg/L 5 (3.5-DCP) = 4 mg/L n , 103 cells/mL t , h b) (NIVA BAC 2) 10 3.5- 5.5 (Phaeodactylum tricornutum) KS I ISO 10253 CCAP 1052/1A( : Off Plymouth, ) NIVA BAC 2 . BAC 2 . , CCAP 1052/1A . KS I ISO 10253 BAC 2 , 11 . , . ISO 21 75 mol/m2 s 1.6 d-1 . , ( , , ). , KS I ISO 10253 10 b)