KS J 0012-2010 Class II(laminar flow) biosafety cabinetry《二级(层流)生物安全柜》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS J 0012 II( ) KS J 0012:2010 2010 12 24 http:/www.kats.go.krKS J 0012:2010 : ( ) ( ) KT and Yan, X., First, M.W., Rudnick, S.N. “Characteristics of Laskin Nozzle Generated Aerosols,” Proc. 21st Nuclear Air Cleaning Conf., M.W. First, Ed., N.T.I.S., Springf

2、ield, VA. Feb. 1991. p. 116 KS J 0012:2010 9 4.6 50 L 300 . . 4.7 pH 0 14 . 4.8 AGI 30 Ace Glass, Inc., Vineland, NJ, Catalog Number 7540 10 6 1 . 4.9 (281.4) L/min 2 (slit-type) . 4.10 6- MRE Refluxing 6-jet modified MRE-type short-form collision nebulizerWaltham, MA, BGI, Inc. model CN-38 Nebulize

3、r( NSF CN 31/l) (nebulizer) . C . 4.11 63 mm , . 4.12 (370.5) . 4.13 (53) . 4.14 . 4.14 (deep freezer) ( 7010) . 4.13 . 4.15 (370.5) . 4.16 (water bath) (650.5) . KS J 0012:2010 10 4.17 5 % . 4.18 0.015 m/s 3 % . 4.19 (direct inflow measurement instrument) 210 3m3/s 3 % B . 4.20 (pitot tube) . Skoki

4、e, Illinois, Alnor Instrument Company 6006 AP 6006 BP . 4.21 , 0 500 Pa, 5 Pa . 4.22 0.5 mm . 4.23 220 mL 10 2 500 rpm 150 mL . 4.24 4.25 100 kg 23 kg . 4.25 1 kg 2 kg 25 cm25 cm . 4.26 . 4.27 , . KS J 0012:2010 11 5 5.1 Bacillus subtilis var. niger(B.subtilis) K.C.T.C. 1022 A.T.C.C. 9372 N.C.T.C. 1

5、0073 5.2 KH2PO4 500 g 5.3 1 N NaOH 100 mL 5.4 5 L 5.5 (MgSO4 7H2O) 500 g 5.6 Nutrient agar trypticase soy agar, (100 mm15 mm 150 mm22 mm) 5.7 (tryptose broth) 500 g 6 II( ) . , . , . 7 7.1 A . A.1 A.13 . . A 7.2 . 7.2 II A 1 . KS J 0012:2010 12 1 II ( A ) / / A.1.1.3 A.1.1.4 A.1.2 A.1.3 10 % 500 Pa1

6、0 % , . 110 5cc/s 510 7cc/s A.2.3.1 A.2.3.2 0.01 % 0.005 % A.3 57 dBA 67 dBA 57 dBA 67 dBA A.4 650 lx , 430 lx , A.5.3 A.5.4 A.5.5 AGI 30 ： 10 B. subtilis cfu/ ： 5 B. subtilis cfu/ ： 5 B. subtilis cfu/ 36 cm 2 cfu/ A.6.3 A.6.4 A.6.5 A.6.6 10 . 0.2 cm . 0.20 cm A.7.3.1 A.7.3.2 20 % 20 % ( ) ( ) B con

7、current air balancing exhaust values A.8.3.2 A.8.3.3 A.8.3.4 0.025 m/s A1： 0.4 m/s m76 m3/h A2 , B1 , B2： 0.5 m/s 0.3 m 110.44 m3/h KS J 0012:2010 13 1 II ( ) ( A ) / A.9.3.1 A.9.3.2 A.9.3.3 A.9.3.4 . , . . . A.10 4 L , 1 . (motor)/ (blower) A.11 10 % . A.12 . A.13 3 . 1 . 7.3 , (0.015 m/s). 7.4 , .

8、 8 , , . . . a) ： / / b) c) d) ： A1 /A2 /B1 /B2 e) f) g) h) ： , , , KS J 0012:2010 14 i) ： A . j) ： , , , ( ) , k) l) , KS J 0012:2010 15 A ( ) , (0.015 m/s). . . 1.3 . D . A.1 / / , . . A.1.1 A.1.1.1 , , . A.1.1.2 , , 0 500 Pa(0 2 in), 5 Pa(0.02 in w.g.) , 0.5 mm(0.02 in) A.1.1.3 ( ) a) . , . b) .

9、c) , ( F.1a ). d) 500 Pa(2 in w.g.) . 30 . 10 % . 500 Pa (2 in w.g.) . A.1.1.4 ( ) KS J 0012:2010 16 a) . , . b) . c) , ( F.1a ). d) 500 Pa(2 in w.g.)10 % . e) , , . . A.1.1.5 500 Pa(2 in w.g.) 30 10 % 500 Pa(2 in w.g.)10 % , . A.1.2 A.1.2.1 , , . A.1.2.2 . A.1.2.3 a) , . . . b) (A.1.1 ). c) 500 Pa(

10、2 in w.g.) . 30 10 % . , (A.1.1 ). d) 15 % , 500 Pa(2 in w.g.) . 15 % , , 500 Pa (2 in w.g.) . e) 30 . f) 110 5cc/s . g) , , , KS J 0012:2010 17 . . 2.5 cm/s(1.0 in/s) 6.313 mm(0.25 0.50 in.) ( F.1b ). A.1.2.4 ( ) 15 % 500 Pa(2 in w.g.) 110 5cc/s . A.1.3 (Sulfur hexafluoride)(SF6) A.1.3.1 , . A.1.3.

11、2 SF6 Ion Track Inc.(ITI) 110 7cc/s SF6 . A.1.3.3 a) , . . . b) (A.1.1 ). c) 500 Pa(2 in w.g.) . 30 10 % . , (A.1.1 ). d) SF6 500 Pa(2 in w.g.) . e) 30 . f) SF6 510 7cc/s . g) , , , . . 2.5 cm/s(1.0 in/s) 6.313 mm(0.25 0.50 in) ( F.1b ). A.1.3.4 (SF6 ) 510 7cc/s . A.2 (HEPA filter leak test) KS J 00

12、12:2010 18 . A.2.1 , , . B1 , 0.015 m/s (3.0 ft/min) . A.2.2 A.2.2.1 . 4.4 . IEST RP CC 013( ) . A.2.2.2 F.2 (Laskin nozzle) DOP . 140 kPa (20 psi) . 2.5 cm(1 in) . A.2.2.3 550 kPa(0 80 psi) , 7 kPa(1 psi) . A.2.3 A.2.3.1 a) (A1/A2 , B2 ). . . . , T . . b) . c) , 10 g/L DOP . (0 100 ), 100 % 100 . ,

13、 1104 . d) 2.5 cm(1.0 in) , . . 5 cm/s(2 in/s) . A.2.3.2 1 cm(0.3 in) KS J 0012:2010 19 . A.2.4 A.2.4.1 0.01 % . A.2.4.2 0.005 % . A.3 . A.3.1 . . 0.015 m/s(3.0 ft/min) . A.3.2 50 100 dB , 1 dB 1 dB “A” weighting scale . A.3.3 a) . b) “A” weighting . c) 30 cm(12 in) 38 cm(15 in) ( F.3 ). d) , c) . A

14、.3.4 57 dBA 67 dBA . 57 dBA , A.3.3 c) . . KS J 0012:2010 20 A.1 dBA 0 2 3 3 4 5 2 6 10 1 10 0 A.4 . A.4.1 lx(foot candles) . A.4.2 (Illuminating Engineering Society： IES) Lighting Handbook (photoelectric illumination meter) 10 % . . A.4.3 a) 30 cm(12 in) , 15 cm(6.0 in) ( F.4 ). b) . c) 30 cm(12 in

15、) , 15 cm(6.0 in) ( F.4 ). A.4.4 650 lx(60 ft-candles) , (11050) lx(105) ft-candles , 430 lx(40 ft-candles) . A.5 , , A.7 . A.5.1 , , . . 30 , . A.7 A.8 . KS J 0012:2010 21 A.5.2 Bacillus subtilis var. niger(B. subtilis) , K.C.T.C. 1022 A.T.C.C. 9372 N.C.T.C. 10073 a.1) (PBS)(1 ) KH2PO434 g 500 mL . 25 (77 F) 1 N NaOH pH 7.20.5 . 1 L . a.2) PBS(2 ) 1 L PBS(1 ) 1.25 mL pH 7.20.5 121 (250 F) 15 . (50 g MgSO4 7H2O/ 1 L) 5.0 mL b) 1 L 25 (77 F) pH 7.00.1 . 121 (250 F) 15 . b) . 4 (39.2 F) a) . Nutrient agar trypticase soy agar,