1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS J 4204 KS J 4204:2008 2008 11 7 http:/www.kats.go.krKS J 4204:2008 : ( ) ( ) KT&G ( ) : (http:/www.standard.go.kr) : :1999 12 31 :2008 11 7 2008-0743 : : ( 025097266) (http:/www.kats.go.kr). 10 5 , . KS J 4204:2008 i 1 1 2 1 3 .1 4 .1 5 1 5.1 ( ).2 5.2 2
2、5.3 2 6 .2 7 .2 7.1 .2 7.2 3 7.3 .3 7.4 .3 8 .3 8.1 .3 8.2 3 8.3 .4 8.4 .5 9 .5 9.1 .5 9.2 5 9.3 .5 9.4 .5 10 6 10.1 6 10.2 .6 KS J 4204:2008 Methods for quantitative analysis of glucose oxidase 1 . (International Union of Biochemistry) EC 1.1.3.4 . 2 . . ( ) . KS I 3206, KS M 0001, KS M 0033, KS M
3、0126, ( ) KS M 8067, ( ) (2 )( ) KS M 8507, (VI) (2 )( ) KS M 8526, ( ) KS M ISO 6353 2, 2: 1 KS M ISO 6353 3, 3: 2 3 KS M 0126 . 4 KS M 0001 . KS I 3206 3. h) 1)( ) . 5 (: Lowrys Method), . KS J 4204:2008 2 5.1 ( ) . 100 mg/L 1 000 mg/L . 2.5 mmol/L , 15 mmol/L 0.5 mol/L . 5.2 220 nm . 10 mg/L 400
4、mg/L . 0.2 mol/L , 50 mmol/L 3 mmol/L . 5.3 pH . 4 mg/L 40 mg/L . . 6 . a) . b) 1.0 g/L . 7 7.1 a) A 50 B 1 . A KS M ISO 6353 2(R34) 0.43 g, KS M ISO 6353 2(R30) ( ) 2.0 g 100 mL . B KS M ISO 6353 2(R9) (II)(5 ) 0.5 g (10 g/L)1) , KS M ISO 6353 3(R24) (0.5 mol/L) pH 6.3 100 mL . b) (2 ) 100 g, KS M
5、8507 25 g 1) (0.5 ) 1 g 100 mL . KS M 8067 (2 ) 1 g . KS J 4204:2008 3 , KS M ISO 6353 2(R22) 50 mL( 0.7 mol), KS M ISO 6353 2(R13) 100 mL( 1.2 mol) 700 mL 10 . 150 g, 50 mL KS M ISO 6353 3(R51) 15 1 000 mL . 0.1 mol/L , 1 mol/L .2)c) 6. . 7.2 a) b) 7.3 a) 0.2 mL 4.0 mL 10 . b) 0.4 mL 30 . c) a) a)
6、b) . d) 10 mm 750 nm . e) f) . f) 0 mg/L 1 000 mg/L 3) 0.1 mL a) b) . . 7.4 100103=SDmAi A : (%) S : (g) D : mi : (mg) 8 8.1 a) , . b) 6. . 8.2 2) 1.9 2 . . 3) 5 . KS J 4204:2008 4 a) 1) 190 nm 350 nm 2) , 3) b) 25 L 100 L 8.3 a) . . 1) 5105 2) 7.8 mm, 30 cm, 3) 4) KS M ISO 6353 2(R35) 48.3 g, (2 )
7、7.8 g 800 mL (0.1 mol/L) pH 7.0 1 000 mL . 5) 1.0 mL/min 6) 10 L 7) 220 nm b) . a) 1 . 1 c) . KS M 0033 8.3 a)( ) 8.7( ) . d) f) . e) KS M 0033 8.4( ) . ( ) 400 mg/L 10 L 0.08 AUFS TSKgelG3000SWXL0 5 10 15KS J 4204:2008 5 f) 0 mg/L 400 mg/L , b) c) . 8.4 100103=SDmAi A : (%) S : (g) D : mi : (mg) 9
8、9.1 a) (0.1 mol/L, pH 7.0) KS M 8526 14.20 g 1 000 mL KS M ISO 6353 3(R79) 13.07 g 1 000 mL pH 7.0 . o- 43 mg/L 5 . b) D- 18.0 g 100 mL . c) (100 U 1.7 kat) 10.0 mg (2.4 mol/L) 10 mL . EC 1.11.1.7 . 9.2 a) b) (250.5) . 9.3 a) 0.5 mL o- 2.5 mL (250.5) 5 . b) ( 4 mg/L 40 mg/L .) 20 L, 10 L 5 . c) 10 m
9、m 436 nm . d) e) . e) 0 mg/L 40 mg/L , 20 L a), b), c) . 9.4 100103=SDmAiKS J 4204:2008 6 A : (%) S : (g) D : mi : (mg) 10 10.1 a) b) c) (%) 10.2 2 . 135513 7017 (02)60094114 (02)600948878 http:/ KS J 4204:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Methods for quantitative analysis of glucose oxidase ICS 71.040.40 Korean Agency for Technology and Standards http:/www.kats.go.kr
copyright@ 2008-2019 麦多课文库(www.mydoc123.com)网站版权所有
备案/许可证编号:苏ICP备17064731号-1