KS J ISO 4831-2007 Microbiology of food and animal feeding stuffs-Horizontal method for the detection and enumeration of coliforms-Most probable number technique《食品和动物饲料微生物学 大肠杆菌菌落.pdf

1、 KS J ISO 4831 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS (MPN) KS J ISO 4831 ：2007 (2012 ) 2007 11 23 http:/www.kats.go.krKS J ISO 4831：2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) ： ：2002 10 31 ：2007 11 23 ：2012 12 28 ： 20120814 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO

2、 4831：2007 (2012 ) (MPN) Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of coliforms Most probable number technique 2006 3 ISO 4831, Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of coliformsMost

3、probable number technique , . ISO 4831 ISO/TC34 (SC9) . 1 . . 30 37 (MPN) . . , 30 . 1 mL 1 g 1 100 . . 11. . 2 . . ( ) . KS H ISO 6887( ), , 10 KS H ISO 7218, ISO 8261, , , 10 KS J ISO 4831：2007 ISO/TS 111331, 1 ： ISO/TS 111332：2003, 2 ： 3 . 3.1 (coliforms) (, 30 37 ) 3.2 , . 3.3 , 1 mL 1 g 4 4.1 4

4、.1.1 , ( ) 30 37 24 48 . 4.1.2 / , 4.1.1 ( ) 30 37 24 48 . 4.1.3 4.1.2 / , . 4.2 MPN 4.2.1 2 3 . 4.2.2 2 3 . , 10 . 4.2.3 2 30 37 ( ) 24 , 24 48 . 2 KS J ISO 4831：2007 4.2.4 2 . 4.2.5 4.2.4 30 37 ( ) 24 48 . 4.2.6 (4.2.5) , mL g (, MPN) . . 5 5.1 , KS H ISO 7218, ISO/TS 111331 ISO/TS 111332 . 5.2 KS

5、 H ISO 6887( ), ISO 8261, . 5.3 ： 5.3.1 a) 2 b) (enzymatic digest of milk and animal proteins) 40 g 20 g (lactose) (C 12 H 22 O 11 H 2 O) 10 g 5 g (dipotassium hydrogen phosphate) (K 2 HPO 4 ) 5.5 g 2.75 g (potassium dihydrogen phosphate) (KH 2 PO 4 ) 5.5 g 2.75 g (sodium chloride) 10 g 5 g (sodium

6、lauryl sulfate) 0.2 g 0.1 g 1 000 mL 1 000 mL 5.3.2 . 25 pH 6.80.2 . (6.5) 16 mm160 mm (6.4), 2 (6.5) 20 mm200 mm (6.4) 10 mL . 121 15 . . 3 KS J ISO 4831：2007 5.3.3 , ISO/TS 111331 . ISO/TS 111332：2003, B.1 . 5.4 ： (brilliant green lactose bile broth) 5.4.1 (enzymatic digest of casein) 10 g (lactos

7、e) (C 12 H 22 O 11 H 2 O) 10 g (dehydrated ox bile) 20 g (brilliant green) 0.013 3 g 1 000 mL 5.4.2 . 25 pH 7.20.2 . (6.5) 16 mm160 mm (6.4) 10 mL . 121 15 . . 5.4.3 , ISO/TS 111331 . ISO/TS 111332：2003, B.1 . 6 (KS H ISO 7218 ) , . 6.1 () ( ) KS H ISO 7218 . 6.2 (301) (371) . 6.3 3 mm 1 6.4 16 mm16

8、0 mm 20 mm200 mm . 4 KS J ISO 4831：2007 6.5 16 mm160 mm(6.4) . 6.6 1 mL 10 mL 6.7 pH 25 0.1 pH . 7 . . 8 KS H ISO 6887( ), ISO 8261 . . 9 ( A ) 9.1 ( A.1 ) 9.1.1 KS H ISO 6887( ), ISO 8261 . 9.1.2 9.1.2.1 x mL, 10 mL . 1 mL x 10 mL 10 mL 5.3.1(a) , x 1 mL 10 mL 5.3.1(b) . 9.1.2.2 2 (9.1.2.1) 30 37 (

9、 ) (6.2) 242 . 9.1.2.3 (9.1.2.1) 30 37 ( ) (6.2) 242 . , 242 . 9.1.3 ( A.3 ) 9.1.3.1 9.1.2.2 (5.4) (6.3) . 482 , 30 37 ( ) 242 . 5 KS J ISO 4831：2007 9.1.3.2 (, 242 482 ) 9.1.2.3 9.1.3.1 . 9.1.4 ( A.1 ) (242) (482) 9.1.3.1 9.1.3.2 . 9.2 (MPN) ( A.2 ) 9.2.1 , KS H ISO 6887( ), ISO 8261 . , . 9.2.2 9.

10、2.2.1 . , / , ( , ) . MPN , KS H ISO 7218 . 9.2.2.2 2 5.3.1(a) . (6.6) 10 mL, 10 mL . 9.2.2.3 5.3.1(b) . (6.6) 1 mL, 1 mL . 9.2.2.4 9.2.2.3 . . . 9.2.2.5 2 (9.2.2.2) 30 37 ( ) (6.2) 242 . 9.2.2.6 (9.2.2.3 9.2.2.4) 30 37 ( ) (6.2) 242 . , 242 . 9.2.3 ( A.3 ) 9.2.3.1 9.2.2.5 (5.4) (6.3) . 30 37 ( ) (6

11、.2) 242 . 482 , 30 37 ( ) 242 . 6 KS J ISO 4831：2007 9.2.3.2 , 9.2.2.6 , ( , 242 482 ), 9.2.3.1 . 9.2.4 ( A.2 ) 242 ( ) 482 , 9.2.3( ) . 10 (9.1.4 ) x g x mL (KS H ISO 7218 ). (KS H ISO 7218 ). 11 . KS H ISO 7218 . 12 . , , 7 KS J ISO 4831：2007 A () () () 10 mL 1 mL 2 1 (9.1.2.1) 1 (9.1.2.1) 24 (9.1

12、.2.2) 24 48 (9.1.2.3) (9.1.3.1) 24 48 (9.1.3.2) (9.1.4) 24 48 (9.1.4) A.1 8 KS J ISO 4831：2007 () () 10 mL 1 mL 2 3 (9.2.2.2) 3 (9.2.2.3) 24 (9.2.2.5) 24 48 (9.2.2.6) (9.2.3.1) 24 48 (9.2.3.1) (9.2.3.2) (9.2.4) 24 48 (9.2.3.2) (9.2.4) A.2 9 KS J ISO 4831：2007 () (+) 5.3 b) 2 5.3 a) (6.3) A.3 10 KS J

13、 ISO 4831：2007 11 1 KS H ISO 4832, 2 EDWARD, P.R. and EWING, W.H., Identification of Enterobacteriaceae, 3rd edn., Burgess Publishing Company, Minneapolis, Minnesota, USA, 1972 (MPN) 153787 1 145 3(16) (02)26240114 (02)26240148 http:/ KS J ISO 4831 ：2007KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of coliforms Most probable number technique ICS 07.100.30 Korean Agency for Technology and Standards http:/www.kats.go.kr