KS J ISO 4832-2007 Microbiology of food and animal feeding stuffs－Horizontal method for the enumeration of coliforms－Colony-count technique《食品和动物饲料的微生物学 大肠杆菌菌落计数水平办法 菌落计数技术》.pdf

1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS J ISO 4832 KS J ISO 4832 ：2007 (2012 ) 2007 10 31 http:/www.kats.go.krKS J ISO 4832：2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) ： ：2002 10 31 ：2007 10 31 ：2012 12 28 ： 20120814 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 4832：

2、2007 (2012 ) Microbiology of food and animal feeding stuffsHorizontal method for the enumeration of coliformsColony-count technique 2006 3 ISO 4832, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coliformsColony-count technique , . ISO 4832 ISO/TC34 (SC9) . 1

3、 30 37 . . . 30 . mL g 100 . 2 . . ( ) . KS H ISO 6887( ), , 10 KS H ISO 7218, ISO 8261：2001, Milk and milk productsGeneral guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination ISO/TS 111331：2000, Microbiology of food and animal feed

4、ing stuffsGuidelines on preparation and production of culture mediaPart 1：General guidelines on quality assurance for the preparation of culture media in the laboratory ISO/TS 111332：2003, Microbiology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 2：P

5、ractical guidelines on performance testing of culture media KS J ISO 4832：2007 3 . 3.1 (30 37 ) VRBL (crystal violet neutral red bile lactose agar) . 4 4.1 2 . 10 2 . 4.2 30 37 24 . 4.3 . . 4.4 mL g (KS H ISO 7218 ). 5 5.1 , KS H ISO 7218, ISO/TS 111331 ISO/TS 11133 2 . 5.2 KS H ISO 6887( ), ISO 826

6、1 . 5.3 VRBL 5.3.1 2 KS J ISO 4832：2007 (enzymatic digest of animal tissues) 7 g (yeast extract) 3 g (lactose)(C 12 H 22 O 11 H 2 O) 10 g (NaCl) 5 g (bile salts) 1.5 g (neutral red) 0.03 g (crystal violet) 0.002 g (agar)a1218 g 1 000 mL a . 5.3.2 . . 25 pH 7.40.2 pH . . 2 4547 (6.5 ) . , . (9.2.2 ).

7、 4 . 5.3.3 ISO/TS 111331 . VRBL ISO/TS 111332：2003 B.1 . 5.4 brilliant green lactose bile broth 5.4.1 (enzymatic digest of casein) 10 g (lactose)(C 12 H 22 O 11 H 2 O) 10 g (dehydrated ox bile) 20 g brilliant green 0.013 3 g 1 000 mL 5.4.2 (6.5 ) . , 25 pH 7.20.2 . 3 KS J ISO 4832：2007 (Durham tube)

8、(6.8 ) (6.7 ) 10 mL . 121 (6.1 ) 15 . . 6 (KS H ISO 7218 ) . 6.1 () ( ) KS H ISO 7218 . 6.2 (301) (371) . 6.3 90100 mm . 6.4 (total-delivery pipettes) 1 mL . 6.5 4447 100 . 6.6 . 6.7 16 mm160 mm . 6.8 (6.7 ) . 6.9 . 6.10 pH 25 pH 0.1 . 6.11 (loop) 4 KS J ISO 4832：2007 3 mm . 7 . , . 8 KS H ISO 6887(

9、), ISO 8261 . , . 9 9.1 , KS H ISO 6887(), ISO 8261 , . 9.2 9.2.1 , 2 . (6.4 ) 1 mL, 1 mL . . 9.2.2 4447 VRBL (5.3 ) 15 mL . ( 10 1 ) 1 5 . . 15 mL . 9.2.3 4447 VRBL (5.3 ) 4 mL . . 9.2.4 30 37 (6.2 ) (242) . 9.3 (9.2.4 ) 10 150 . (6.6 ) 0.5 mm ( .) . . KS H ISO 7218 . 5 KS J ISO 4832：2007 ( ) 9.4 .

10、 . . 9.4 5 brilliant green lactose bile (5.4 ) . 30 37 (6.2) (242) . . (10. ) . 10 KS H ISO 7218 . 11 (Poisson distribution) 1652 % ( 3 ). . (s r ) 0.20 log , (s R ) 0.35 log ( 4 5 ). KS H ISO 7218 . 12 . ( ) 6 KS J ISO 4832：2007 7 A () 1 KS H ISO 4831, (MPN) 2 EDWARD, P.R. and EWING, W.H. Identific

11、ation of Enterobacteriaceae 3rd edition, Burgess Publishing Company, Minneapolis, Minnesota, USA, 1972 3 COWELL and MORISETTI. J. Sci. Food Agric. 20, 1969, pp. 573 4 PITON and GRAPPIN. J. Assoc. Anal. Chem. 74, 1991, pp. 92103 5 ALDRIDGE et al. Report of the Ministry of Agriculture, Fish and Food,

12、Norwich, NR47UQ, 1993 153787 1 145 3(16) (02)26240114 (02)26240148 http:/ KS J ISO 4832 ：2007 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Microbiolog of food and animal y feeding stuffs Horizontal method for the enumeration of coliforms Colony-count technique ICS 07.100.30 Korean Agency for Technology and Standards http:/www.kats.go.kr