KS J ISO 7251-2007 Microbiology of food and animal feeding stuffs-Horizontal method for the detection and enumeration of presumptive Escherichia coli -Most probable number techniqu.pdf

1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS J ISO 7251 KS J ISO 7251 ：2007 (2012 ) 2007 10 31 http:/www.kats.go.krKS J ISO 7251：2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) ： ：2002 10 31 ：2007 10 31 ：2012 12 28 ： 20120814 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 7251：

2、2007 (2012 ) Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of presumptive Escherichia coli Most probable number technique 2005 3 ISO 7251, Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of presump

3、tive Escherichia coliMost probable number technique , . ISO 4832 ISO/TC34 (SC9) . 1 37 44 (MPN) . . 44 . (10. ). 2 . . ( ) . KS H ISO 68871, , 10 1： 10 KS H ISO 68872, , 10 2： KS H ISO 68873, , 10 3： KS H ISO 68874, , 10 4： , , KS J ISO 7251：2007 KS H ISO 7218, ISO 8261, Milk and milk productsGenera

4、l guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination ISO/TS 111331：2000, Microbiology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 1：General guidelines on quality assurance for the

5、preparation of culture media in the laboratory ISO/TS 111332：2003, Microbiology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 2：Practical guidelines on performance testing of culture media 3 . 3.1 (presumptive Escherichia coli) 44 44 3.2 , mL g 4 4.1

6、4.1.1 . 4.1.2 37 48 . 24 48 . 4.1.3 , (EC ) 2 . 4.1.4 4.1.3 44 48 . 24 48 . 4.1.5 4.1.4 , (indol-free pepton wa ter) 2 . 4.1.6 4.1.5 44 48 . . 4.1.7 (4.1.1 ) 2 EC 44 . x g x mL “ ” “ ” . 4.2 2 KS J ISO 7251：2007 4.2.1 . 4.2.2 . , 10 . 4.2.3 37 48 . 24 48 . 4.2.4 , (EC ) 2 . 4.2.5 4.2.4 44 48 . 24 48

7、 . 4.2.6 4.2.5 , 2 . 4.2.7 4.2.6 44 48 . . 4.2.8 2 EC 44 MPN ( A ) . 5 , KS H ISO 7218 . 5.1 KS H ISO 68871 , ISO 8261 . 5.2 ( )(lauryl sulfate broth) 5.2.1 a) b) (enzymatic digest of plant and animal tissues) 40.0 g 20.0 g (lactose) 10.0 g 5.0 g (K 2 HPO 4 ) 5.5 g 2.75 g (KH 2 PO 4 ) 5.5 g 2.75 g (

8、sodium chloride) 10.0 g 5.0 g CH 3 (CH 2 ) 11 OSO 3 Na 0.2 g 0.1 g 1 000 mL 1 000 mL 5.2.2 3 KS J ISO 7251：2007 . 25 pH 6.80.2 pH . (6.6 ) 16 mm160 mm (6.4 ) 9 mL , (6.6 ) 18 mm180 mm 20 mm200 mm (6.4 ) 10 mL . 121 (6.1 ) 15 . . 5.3 EC ( ) 5.3.1 (enzymatic digest of casein) 20.0 g (lactose) 5.0 g 3(

9、bile salts No. 3) a1.5 g (K 2 HPO 4 ) 4.0 g (KH 2 PO 4 ) 1.5 g (sodium chloride) 5.0 g 1 000 mL a 1 5.3.2 . 25 pH 6.80.2 pH . (6.6 ) 16 mm160 mm (6.4 ) 10 mL . 121 (6.1 ) 15 . . 5.3.3 , ISO/TS 111331 ISO/TS 111332 . 5.4 (peptone water, indole free) 5.4.1 (enzymatic digest of casein) 10.0 g (sodium c

10、hloride) 5.0 g 1 000 mL 4 KS J ISO 7251：2007 5.4.2 . 25 pH 7.30.2 pH . 16 mm160 mm (6.4 ) 5 mL10 mL . 121 (6.1 ) 15 . 5.5 (Kovacs ) 5.5.1 4(4dimethylaminobenzaldehyde) 5.0 g 21 1(2methylbutan1ol or pentan1ol) 75.0 mL (hydrochloric acid)( 201.181.19 g/mL) 25.0 mL 5.5.2 5055 4 (4 dimethylaminobenzalde

11、hyde) . . 4 (KS H ISO 7218 ). . . 6 , . . 6.1 () ( ) KS H ISO 7218 6.2 (371) (441) . 6.3 (441) . 6.4 5 KS J ISO 7251：2007 16 mm160 mm 18 mm180 mm 20 mm200 mm . 6.5 / / 3 mm 10 L 6.6 (6.4 ) . 6.7 (total-delivery pipettes) 1 mL 10 mL . 6.8 pH 0.01 pH , 25 pH 0.1 . 7 , . . , . 8 . , . 9 9.1 9.1.1 KS H

12、ISO 6887 ISO 8261 . 1 mL 9 mL 5.2.1 b) ( 0.1 g 0.1 mL) , 10 mL 10 mL 5.2.1 a) ( 1 g 1 mL) . , x mL x g 9 x mL (KS H ISO 6887 ISO 8261 ) 90x mL 5.2.1 b) . , 5 mL 5 g 45 mL , 450 mL 5.2.1 b) . 5.2.1 a) . 9.1.2 ( ) 6 KS J ISO 7251：2007 (5.2 ) 37 (6.2 ) 242 . , (482) . (482) . ( , ) , . 48 EC 9.1.3 . 9.

13、1.3 (EC ) 9.1.2 5.2.1 a , , 9.1.2 5.2.1 b , EC (5.3 ) (6.5 ) 44 (6.3 ) (6.2 ) (242) . EC (5.3 ) , (482) . (242) . 9.1.4 9.1.3 , 44 (5.4 ) (6.5 ) . 44 (482) . 9.1.5 0.5 mL (5.5 ) 9.1.4 (5.4 ) . 1 . . 9.1.6 9.1.2 (9.1.3 9.1.4 2 ) EC , . 9.2 9.2.1 , KS H ISO 6887 ISO 8261 . . 9.2.2 ( ) 9.2.2.1 . / ( A

14、) . 7 KS J ISO 7251：2007 9.2.2.2 5.2.1 a) . (6.7 ) 10 mL . 1 g . 9.2.2.3 5.2.1 b) . (6.7 ) 1 mL . 0.1 g . 9.2.2.4 ( 0.01 g, 0.001 g ) 9.2.2.3 . . . 9.2.2.5 9.2.2.2 , 9.2.2.3 9.2.2.4 37 (6.2 ) 242 . , 482 . (482) . ( , ) , . 48 , EC (5.3) 9.2.3 . 9.2.3 (EC ) 2 9.2.3.1 9.2.2.5 (, .) , 9.2.2.5 ( .) EC

15、(5.3 ) (6.5 ) 2 . 9.2.3.2 9.2.3.1 44 (6.3 ) (6.2 ) (242) . EC (5.3 ) , (482) . (242) . 9.2.4 9.2.3.2 , , 44 (5.4 ) (6.5 ) . 44 (482) . 9.2.5 0.5 mL (5.5 ) 9.2.4 (5.4 ) . 1 . . 9.2.6 9.2.2 5.2.1 a) (9.2.3 8 KS J ISO 7251：2007 9.2.4 2 ) EC , . , 5.2.1 a) 5.2.1 b) . 10 10.1 (9.1.6 ) , . g mL . 10.2 A .

16、 95 % , 7.410 MPN 13200 , 2.410 MPN 4 99 . 11 . a) b) , c) d) e) 9 KS J ISO 7251：2007 A () (MPN table) A.1 MPN KS H ISO 7218 . A.2 MPN A.1 . A.1 5 1 g(mL), 50.1 g(mL) 5 0.01 g(mL) () a MPN 1 2 3 5 10 95 % 95 % 99 % 99 % 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 0 0 1 1 2 2 3 0 0 0 1 1

17、1 2 2 3 3 4 0 0 0 1 1 1 2 2 2 3 0 1 0 1 0 1 0 0 1 2 0 1 2 0 1 0 1 0 0 1 2 0 1 2 0 1 2 0 0.18 0.18 0.18 0.36 0.37 0.55 0.56 0.20 0.40 0.60 0.40 0.61 0.81 0.61 0.82 0.83 1.0 1.1 0.45 0.68 0.91 0.68 0.92 1.2 0.93 1.2 1.4 1.2 2 2 3 3 0 0 1 2 0 1 3 0 2 3 3 0 0 1 2 0 1 2 0 1 3 0 3 2 2 3 2 0 3 1 1 0 1 2 0

18、1 3 3 0 0 1 1 3 1 2 0 1 3 0 2 2 2 3 2 0 3 1 1 3 1 2 0 1 3 3 0 0 1 1 3 1 1 3 1 2 0 2 1 1 2 2 3 3 1 1 3 1 2 0 1 3 3 0 0 1 1 3 1 1 3 1 2 0 2 1 1 2 1 3 3 1 1 3 1 1 3 1 2 2 3 3 1 1 3 1 1 3 1 2 3 1 0.00 0.00 0.01 0.07 0.07 0.17 0.17 0.02 0.07 0.17 0.07 0.17 0.33 0.18 0.33 0.33 0.3 0.3 0.08 0.18 0.33 0.19

19、0.33 0.4 0.34 0.4 0.6 0.4 0.65 0.65 0.65 0.99 0.99 1.40 1.40 0.99 1.00 1.40 1.10 1.40 2.20 1.40 2.20 2.20 2.2 2.2 1.4 1.50 2.20 1.70 2.20 2.5 2.20 2.5 3.4 2.5 0.00 0.00 0.00 0.02 0.02 0.09 0.09 0.01 0.02 0.09 0.03 0.09 0.20 0.09 0.20 0.20 0.2 0.2 0.04 0.09 0.20 0.10 0.20 0.2 0.20 0.2 0.4 0.2 0.93 0.

20、93 0.93 1.40 1.40 2.10 2.10 1.40 1.40 2.10 1.40 2.10 2.80 2.10 2.80 2.80 2.8 2.8 2.10 2.10 2.80 2.30 2.80 3.4 2.80 3.4 4.4 3.4 10 KS J ISO 7251：2007 A.1 5 1 g(mL), 50.1 g(mL) 5 0.01 g(mL) () () a MPN 1 2 3 5 10 95 % 95 % 99 % 99 % 2 2 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4

21、 4 4 5 5 5 5 5 5 3 4 0 0 0 1 1 1 2 2 2 3 3 3 4 4 5 0 0 0 0 1 1 1 1 2 2 2 2 3 3 3 4 4 4 5 5 5 0 0 0 1 1 1 0 0 1 2 0 1 2 0 1 2 0 1 2 0 1 0 0 1 2 3 0 1 2 3 0 1 2 3 0 1 2 0 1 2 0 1 0 1 2 3 0 1 1.4 1.5 0.78 1.1 1.3 1.1 1.4 1.7 1.4 1.7 2.0 1.7 2.1 2.4 2.1 2.4 2.5 1.3 1.7 2.1 2.5 1.7 2.1 2.6 3.1 2.2 2.6 3.

22、2 3.8 2.7 3.3 3.9 3.4 4.0 4.7 4.1 4.8 2.3 3.1 4.3 5.8 3.3 4.6 0 0 1 1 3 1 2 3 1 2 0 2 3 0 3 0 0 1 1 3 0 1 1 3 0 1 2 3 0 1 2 3 2 3 0 3 0 1 1 3 0 1 1 3 3 1 1 3 1 1 3 1 2 3 2 3 0 3 3 0 1 1 2 0 1 1 3 0 1 1 3 0 1 2 3 2 3 0 3 0 1 1 2 0 1 1 3 3 1 1 3 1 1 3 1 2 3 1 3 0 2 3 0 1 1 2 0 1 1 2 0 1 1 3 0 1 1 3 1

23、2 0 3 3 1 1 2 0 1 1 3 3 1 1 2 1 1 3 1 1 3 1 2 3 2 3 3 1 1 2 0 1 1 2 0 1 1 2 0 1 1 3 1 2 3 3 3 1 1 2 3 1 1 3 3 1 1 2 1 1 2 1 1 3 1 2 3 2 3 3 1 1 2 3 1 1 2 3 1 1 2 3 1 1 2 1 2 3 2 3 1 1 1 3 1 1 0.6 0.6 0.21 0.4 0.6 0.4 0.6 0.6 0.6 0.7 0.7 0.7 0.7 1.0 0.7 1.0 1.0 0.4 0.5 0.7 1.0 0.6 0.7 1.0 1.0 0.7 1.0

24、 1.0 1.3 1.0 1.0 1.3 1.3 1.3 1.4 1.3 1.4 0.7 1.0 0.3 2.1 1.0 1.4 3.4 3.4 2.20 2.2 3.4 2.5 3.4 3.4 3.4 3.9 3.9 3.9 3.9 6.6 4.0 6.6 6.6 3.4 3.4 3.9 6.6 3.9 4.1 6.6 6.6 4.8 6.6 6.6 10.0 6.6 6.6 10.0 10.0 10.0 11.3 10.0 11.3 6.6 6.6 10.0 14.9 10.0 11.3 0.4 0.4 0.12 0.2 0.4 0.2 0.4 0.4 0.4 0.5 0.5 0.5 0.

25、5 0.7 0.5 0.7 0.7 0.3 0.4 0.5 0.7 0.4 0.5 0.7 0.7 0.5 0.7 0.7 0.9 0.7 0.7 0.9 0.9 0.9 0.9 0.9 0.9 0.5 0.7 0.9 1.4 0.7 0.9 4.4 4.4 2.80 2.9 4.4 3.4 4.4 4.4 4.4 5.1 5.2 5.2 5.2 9.4 5.2 9.4 9.4 4.4 4.4 5.2 9.4 5.1 5.3 9.4 9.4 6.1 9.4 9.4 14.7 9.4 9.4 14.7 14.7 14.7 14.7 14.7 14.7 9.4 9.4 14.7 20.0 14.7 14.7 11 KS J ISO 7251：2007 A.1 5 1 g(mL), 50.1 g(mL) 5 0.01 g(mL) () () a MPN 1 2 3 5 10 95 % 95 % 99 % 99 % 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 1 1 2 2 2 2 2 3 3 3 3 3 4