KS J ISO 7932-2007 Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of presumptive Bacillus cereus-Colony-count technique at 30 ℃《食品和动物饲料的微生物学 蜡.pdf

1、 KS J ISO 7932 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 30 KS J ISO 7932 ：2007 (2012 ) 2007 11 23 http:/www.kats.go.krKS J ISO 7932：2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) ： ：2002 10 31 ：2007 11 23 ：2012 12 28 ： 20120814 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 79

2、32：2007 (2012 ) 30 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus Colony-count technique at 30 2004 3 ISO 7932, Microbiology of food and animal feeding stuffsHorizontal method for the enumeration of presumptive Bacillus cereusColon

3、y-count technique at 30 , . ISO 7932 ISO/TC34 (SC9) . 1 30 . . , MYP . “ ” (B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides) . . B. cereus from B. anthracis . 2 . . ( ) . KS H ISO 68871：1999, , 10 1： 10 KS H ISO 7218：1996, 1 ：2001 ISO/TS 111332：2003, Microbiology of food and anima

4、l feeding stuffsGuidelines on preparation and production of culture mediaPart 2：Practical guidelines on performance testing of culture media KS J ISO 7932：2007 3 . 3.1 , 1. . 4 4.1 , . 10 . 4.2 30 1848 . 4.3 g mL . 5 , KS H ISO 7218 . . 5.1 KS H ISO 68871 . 5.2 (agar medium) ( 1 ) 5.2.1 5.2.1.1 (bee

5、f extract) 1.0 g (enzymaic digest of casein) 10.0 g D-(D-Mannitol) 10.0 g (NaCl) 10.0 g (phenol red) 0.025 g (agar) 12 g18 g a 900 mL a . 2 KS J ISO 7932：2007 5.2.1.2 . , 25 (5.2.4) pH 7.20.2 . 90 mL . 121 (6.1) 15 . 5.2.2 B 5.2.2.1 B (polymyxin B sulfate) 10 6IU 100 mL 5.2.2.2 B . . 5.2.3 . . 95 %

6、(V/V) 30 . . 4 . . 44 47 (6.4) 2 , (53) 1824 . . (53) 72 . 5.2.4 (MYP ) 5.2.4.1 (base medium) (5.2.1) 90 mL B (polymyxin B solution) (5.2.2) 1.0 mL (egg yolk emulsion) (5.2.3) 10.0 mL 5.2.4.2 44 47 (6.4) . . 3 KS J ISO 7932：2007 44 47 (6.4) . 5.2.5 (6.6) (5.2.4) 1520 mL . (53) 4 . 37 55 (6.2) . . 5.

7、2.6 ISO/TS 111332：2003 B . 5.3 (sheep blood agar) 5.3.1 ： 2(base medium : blood agar base No.2) 5.3.1.1 (proteose peptone or equivalent peptone) 15 g (liver hydrolysate) 2.5 g (yeast extract) 5 g (NaCl) 5 g (agar) 12 g18 g a 1 000 mL a . 5.3.1.2 . , 25 pH 7.00.2 . 121 15 . 5.3.2 (defibrinated sheep

8、blood) 5.3.2.1 5.3.2.1.1 (5.3.1) 100 mL (defibrinated sheep blood) 5 mL7 mL 4 KS J ISO 7932：2007 5.3.2.2 44 47 (5.3.1) . 12 mL (6.6) . 6 1 . . 6.1 () ( ) KS H ISO 7218 . 6.2 (還流) (371) (551) . 6.3 (301) . 6.4 44 47 . 6.5 pH- 25 0.1 pH . 6.6 90 mm100 mm( 140 mm) . 6.7 . 10 mL 1 mL . 0.1 mL 0.5 mL . 2

9、 mm3 mm . 6.8 ( ), 3.5 mm 20 cm . 3 cm . . 7 . . . 5 KS J ISO 7932：2007 8 . . 9 9.1 . KS H ISO 68871 . 9.2 9.2.1 (6.7) 0.1 mL 0.1 mL (5.2.5) . 10 . 9.2.2 , 1.0 mL 1.0 mL 10 . (6.8) (140 mm) 3 (90 mm) 1 mL . 6 . 9.2.3 (6.8) . . 15 . 9.2.4 (9.2.3) 30 (6.3) 1824 . 24 . 9.3 (9.2.4) 2 150 . . ( . 1 ) (le

10、cithinase) . 2 . 15 , 10. . 1 . 2 . . . 1.0 mL 3 (9.2.2 ) 6 KS J ISO 7932：2007 . 9.4 9.4.1 9.3 5 . 5 . 9.4.2 9.4.3 . (5.2.4) 5 . 30 (6.3) 1824 . . 9.4.2 9.4.3 . 9.4.2 (9.4.1) (5.3) , . 30 (242) . 9.4.3 1 . 1 MYP (9.4.1) (9.3 1 ) (9.4.2) aa . 10 10.1 KS H ISO 7218 . 10.2 ( ) ( ) . mL( ) 1 7 KS J ISO

11、7932：2007 g ( ) 1/d d . 10.3 10.3.1 B (7 8 ). . . 10.3.2 (log 10 -) ( ) 5 % r . (r) . r = 0.29(log 10 - ) r = 2.0( ) (4 ) . r = 0.12(log 10 - ) r = 1.3( ) 1 10 000 1.010 4 . 2.0 . 5 000(=10 000/2.0) 20 000(=10 0002.0) . 10.3.3 (log 10 -) ( ) 5 % R . (R) . R = 0.42(log 10 - ) R = 2.6( ) 8 KS J ISO 79

12、32：2007 (4 ) . R = 0.23(log 10 - ) R = 1.7( ) 1 1 10 000 1.010 4 . 2.6 . 3 800(=10 000/ 2.6) 26 000(=10 0002.6) . 2 ( 100 000 log 10 5 ) . R ( ) 0.59 . log10 0.25(0.420.59) 1.78(10 0.25 ). log 10 5.25(log 10 5log 10 0.25) 178 000(100 0001.78) . 0.59 95 % . 0.59 . 2 96 . 1 64 . 1 59 . 0 11 . a) b) ,

13、c) d) e) f) ( ) 9 KS J ISO 7932：2007 A () 15 A.1 . A.1 95 % 1 1 2 2 1 4 3 1 5 4 1 6 5 2 9 6 2 10 7 2 12 8 3 13 9 4 14 10 4 16 11 5 18 12 6 19 13 7 20 14 7 21 15 8 23 10 KS J ISO 7932：2007 B () 17 20 , , , . . SMT4 CT96 2098 1997 10 (RIVM) . ISO 7932：1993 . MYP , , VP ISO 57251：1994 5 B.1B.4 . B.1 (

14、) ( ) ( ) ( ) 2 2 2 18 18 18 0 0 0 36 35 36 a (log 10cfu/ g) 3.3 4.7 6.1 s r(log 10cfu/ g) 0.09 0.05 0.10 2.63 1.16 1.60 r： log 10(log 10cfu/ g) 0.24 0.15 0.27 (cfu/ g) 1.7 1.4 1.9 s R(log 10cfu/ g) 0.11 0.09 0.10 (%) 3.24 1.98 1.71 R ： log 10(log 10cfu/ g) 0.30 0.26 0.29 (cfu/ g) 2.0 1.8 2.0 11 KS

15、J ISO 7932：2007 B.2 ( ) ( ) ( ) ( ) 2 2 2 18 18 18 0 0 0 36 36 36 a (log 10cfu/ g) 3.1 3.9 4.9 s r(log 10cfu/ g) 0.13 0.14 0.08 4.15 3.49 1.56 r： log 10(log 10cfu/ g) 0.36 0.38 0.21 (cfu/ g) 2.3 2.4 1.6 s R(log 10cfu/ g) 0.15 0.14 0.11 (%) 4.72 3.49 2.30 R： log 10(log 10cfu/ g) 0.41 0.38 0.31 (cfu/

16、g) 2.5 2.4 2.1 B.3 ( ) ( ) ( ) ( ) 2 2 2 12 12 12 0 0 0 23 23 23 a (log 10cfu/ g) 3.4 4.1 6.2 s r(log 10cfu/ g) 0.05 0.06 0.12 1.50 1.57 1.95 r： log 10(log 10cfu/ g) 0.14 0.18 0.33 (cfu/ g) 1.4 1.5 2.2 s R(log 10cfu/ g) 0.08 0.10 0.17 (%) 2.28 2.38 2.78 R： log 10(log 10cfu/ g) 0.22 0.27 0.48 (cfu/ g

17、) 1.6 1.9 3.0 12 KS J ISO 7932：2007 B.4 2 18 0 36 a (log 10cfu/ capsule) 3.9 s r (log 10cfu/ capsule) 0.04 (%) 1.12 r： log 10(log 10cfu/ capsule) 0.12 (cfu/ capsule) 1.3 s R(log 10cfu/ capsule) 0.08 (%) 2.16 R： log 10(log 10cfu/ capsule) 0.23 (cfu/ capsule) 1.7 13 KS J ISO 7932：2007 14 1 MOSSEL, D.A

18、.A., KOOPMAN, M.J. and JON GERIUS, E. Appl. Microbiol., 15, 1967, pp. 650653 2 ICMSF. Microorganisms in foods, Vol. 1, 2nd edn., University of Toronto Press, 1978 3 COWELL and MORISETTI J. Sci. Fd. Agric., 20, 1969, p. 573 4 INT VELD, P.H., SOENTORO, P.S.S. and NOTERMANS, S.H.W. I. J. Food Microbiol

19、., 20, 1993, pp. 2336 5 ISO 57251：1994, Accuracy (trueness and precision) of measurement methods and resultsPart 1 General principles and definitions 6 ISO 57252：1994, Accuracy (trueness and precision) of measurement methods and resultsPart 2：Basic method for the determination of repeatability and r

20、eproducibility of a standard measurement method 7 SCHULTEN, S.M., VAN de LUSTGRAAF, B.E.B., NAGELKERKE, N.J.D. and IN T VELD, P.H. Report No. 286555001, National Institute of Public Health and the Environment, Bilthoven, The Netherlands, 1998 8 SCHULTEN, S.M., IN T VELD, P.H., NA GELKERKE, N.J.D., S

21、COTTER, S., DE BUYSER, M.L., ROLLIER, P. and LAHELLEC, C. I. J. Food Microbiology, 57, 2000, pp. 5361 30 153787 1 145 3(16) (02)26240114 (02)26240148 http:/ KS J ISO 7932 ：2007KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus Colony-count technique at 30 ICS 07.100.30 Korean Agency for Technology and Standards http:/www.kats.go.kr