1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-23007-4 SANS 111:2009Edition 1 ISO 835:2007Edition 1SOUTH AFRICAN NATIONAL STANDARD Laboratory Glassware Graduated pipettes This national
4、standard is the identical implementation of ISO 835:2007 and is adopted with the permission of the International Organization for Standardization. Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za
5、SABS SANS 111:2009 Edition 1 ISO 835:2007 Edition 1 Table of changes Change No. Date Scope National foreword This South African standard was approved by National Committee SABS TC 48, Laboratory Equipment, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of th
6、e WTO/TBT agreement. This SANS document was published in September 2009. Reference numberISO 835:2007(E)ISO 2007INTERNATIONAL STANDARD ISO835First edition2007-04-01Laboratory glassware Graduated pipettes Verrerie de laboratoire Pipettes gradues SANS 111:2009This s tandard may only be used and printe
7、d by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobes licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to
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11、n in writing from either ISO at the address below or ISOs member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2007 All rights reservedS
12、ANS 111:2009This s tandard may only be used and printed by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) ISO 2007 All rights reserved iiiContents Page Foreword iv 1 Scope . 1 2 Normative references . 1 3 Terms and definitions. 1 4 Basis of adjustment 2 4.1 Unit of volume
13、. 2 4.2 Delivery volume 2 4.3 Reference temperature. 2 5 Types and classes of accuracy. 2 5.1 Classes of accuracy . 2 5.2 Types of pipettes 2 6 Maximum permissible errors. 3 7 Construction 4 7.1 Material 4 7.2 Dimensions 4 7.3 Top of pipette 4 7.4 Delivery jet. 4 7.5 Delivery time 5 7.6 Waiting time
14、. 5 8 Graduation, figuring and patterns. 5 8.1 Graduation patterns 5 8.2 Position of graduation lines 5 8.3 Figuring of graduation lines 5 9 Setting of the meniscus . 6 10 Marking 6 11 Visibility of graduation lines, figures and inscriptions. 7 12 Colour coding 7 Annex A (normative) Definition of ca
15、pacities and delivery times. 8 Bibliography . 12 SANS 111:2009This s tandard may only be used and printed by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) iv ISO 2007 All rights reservedForeword ISO (the International Organization for Standardization) is a worldwide fede
16、ration of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that commi
17、ttee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordan
18、ce with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires
19、approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 835 was prepared by Technical Comm
20、ittee ISO/TC 48, Laboratory equipment, Subcommittee SC 6, Laboratory and volumetric ware. This first edition of ISO 835 cancels and replaces ISO 835-1:1981, ISO 835-2:1981, ISO 835-3:1981 and ISO 835-4:1981, which have been technically revised and combined into one document. SANS 111:2009This s tand
21、ard may only be used and printed by approved subscription and freemailing clients of the SABS .INTERNATIONAL STANDARD ISO 835:2007(E) ISO 2007 All rights reserved 1Laboratory glassware Graduated pipettes 1 Scope This International Standard specifies metrological and constructional requirements for g
22、raduated pipettes, adequate for general laboratory purposes. The details specified are in conformity with the principles of design and construction of volumetric glassware given in ISO 384. NOTE For one-mark pipettes, see ISO 648. For piston-operated pipettes, see ISO 8655-2. 2 Normative references
23、The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 384:1978, Laboratory glassware Principles of
24、design and construction of volumetric glassware ISO 719, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classification ISO 1769, Laboratory glassware Pipettes Colour coding ISO 3696, Water for analytical laboratory use Specification and test methods 3 Terms and definitions Fo
25、r the purposes of this document, the following terms and definitions apply. 3.1 delivery volume volume of liquid discharged from the pipette NOTE Due to retention of liquid on the inner surface of the pipette, the volume of liquid delivered is not identical with the volume of liquid contained by the
26、 pipette. 3.2 delivery time time required for the pipette to deliver its nominal volume 3.3 waiting time time to be observed after apparent completion of the liquid delivery of the pipette and before the final reading of the delivered volume is taken NOTE A waiting time applies for graduated pipette
27、s of Class AS (see 5.1 and 7.6). SANS 111:2009This s tandard may only be used and printed by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) 2 ISO 2007 All rights reserved4 Basis of adjustment 4.1 Unit of volume The unit of volume shall be the millilitre (ml) which is equi
28、valent to the cubic centimetre (cm3). 4.2 Delivery volume Graduated pipettes shall be adjusted with water according to ISO 3696, grade 3, to deliver their specified volume (Ex). The delivery should be under gravity and unrestricted. For details, see 7.5. 4.3 Reference temperature The standard refere
29、nce temperature, i.e. the temperature at which the pipette is intended to deliver its volume, shall be 20 C. When the pipette is required for use in a country which has adopted a standard reference temperature of 27 C (the alternative recommended in ISO 384 for tropical use), this figure shall be su
30、bstituted for 20 C. 5 Types and classes of accuracy 5.1 Classes of accuracy Two classes of accuracy are specified: Classes A and AS for the higher grade; Class B for the lower grade. The maximum permissible errors for both classes are specified in Table 1. The pipettes shall be adjusted as follows:
31、Class A: without waiting time; Class AS: with a specified waiting time of 5 s (see 7.6); Class B: without waiting time. 5.2 Types of pipettes 5.2.1 Type 1: Partial delivery Graduated pipettes adjusted for delivery of a liquid from zero line at the top to any graduation line; nominal capacity shall b
32、e represented by the lowest graduation line. 5.2.2 Type 2: Total delivery Graduated pipettes adjusted for delivery of a liquid from any graduation line down to the jet; nominal capacity shall be represented by the highest graduation line. SANS 111:2009This s tandard may only be used and printed by a
33、pproved subscription and freemailing clients of the SABS .ISO 835:2007(E) ISO 2007 All rights reserved 35.2.3 Type 3: Total delivery Graduated pipettes adjusted for delivery of a liquid from zero line at the top to any graduation line; nominal capacity shall be obtained by delivery down to the jet.
34、5.2.4 Type 4: Blow-out Graduated pipettes for total delivery (see Type 3) where the last drop of liquid in the jet is expelled by blowing. Blow-out pipettes shall be adjusted to accuracy Class B. 6 Maximum permissible errors Maximum permissible errors in the delivered volume shall not exceed the lim
35、its specified in Table 1. These limits represent the maximum permitted error at any point and also the maximum permissible difference between the errors at any two points. Table 1 Capacities, subdivisions and maximum permissible errors Nominal capacity Smallest scale division Maximum permissible err
36、or ml ml Classes A and AS ml Class B ml 0,1 0,01 0,006 0,01 0,2 0,01 0,006 0,01 0,5 0,01 0,006 0,01 1 0,01 0,007 0,01 1 0,10 0,007 0,01 2 0,02 0,010 0,02 2 0,10 0,010 0,02 5 0,05 0,030 0,05 5 0,10 0,030 0,05 10 0,1 0,05 0,1 20 0,1 0,1 0,2 25 a0,1 0,1 0,2 25 0,2 0,1 0,2 aLength 450 mm, see Table 2. I
37、f graduated pipettes are required of capacities and/or subdivisions other than those listed in Table 1, they shall conform to the essential requirements of this International Standard. The relationships between maximum permissible error and capacity, as well as between maximum permissible error and
38、meniscus diameter as given in ISO 384:1978, Annex A and Annex B, shall be observed. SANS 111:2009This s tandard may only be used and printed by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) 4 ISO 2007 All rights reserved7 Construction 7.1 Material Graduated pipettes shal
39、l be manufactured from glass of chemical resistance and thermal properties which are at least to class HGB3 in accordance with ISO 719, and shall be as free as possible from visible defects and shall be reasonably free from internal stress. 7.2 Dimensions Graduated pipettes shall comply with the ess
40、ential dimensional requirements shown in Table 2. Table 2 Dimensions Nominal capacity ml Dimensions mm 0,1 0,2 0,5 1 2 5 10 20 25 25 Smallest scale division ml 0,01 0,01 0,01 0,010,100,020,100,050,100,1 0,1 0,1 0,2 Essential dimensions Distance from zero line to lowest graduation line for Type 1 pip
41、ettes min. max. 80170 120200 130220 160 220 160 220 140220 140 220 140 220 220 290 180220 Distance from highest to lowest figured graduation lines for Types 2, 3 and 4 pipettes min. max. 70160 110190 110 220 140 220 140 220 140220 140 220 140 220 200 290 160 220 Distance from highest graduation line
42、 to top of pipette min. 100 External diameter of suction tube approx. 5,25 to 9 5,5 to 9 Length of tube of uniform bore below lowest graduation line min. 5 Recommended dimensions Overall length approx. 300 340 360 360 360 360 360 360 450 360 Length of tapered portion forming jet approx. 15 15 20 20
43、20 25 25 30 30 30 Wall thickness approx. 2 2 2 2 1,5 1 1 1 1 1 7.3 Top of pipette The top of the graduated pipette shall lie in a plane at right angles to the axis of the pipette and shall be free from any blemishes which might interfere with the required accurate control by a mechanical device or b
44、y the finger, in setting the meniscus. The end may be lightly fire-polished or smoothly ground with a slight bevel on the outside. The upper end of the pipette tube may be made from tubing of uniform bore or may have a constriction at about 25 mm below the top to enable the retention of a porous, ai
45、r-permeable plug, such as a cotton plug. 7.4 Delivery jet The lower end of the graduated pipette shall terminate either in a delivery jet having a smooth and gradual taper or in a capillary end, both without sudden constriction at the orifice which could give rise to turbulent outflow. The end of th
46、e jet may be finished by grinding or machine tooling and may be fire polished. SANS 111:2009This s tandard may only be used and printed by approved subscription and freemailing clients of the SABS .ISO 835:2007(E) ISO 2007 All rights reserved 57.5 Delivery time The delivery time is defined as the ti
47、me occupied by the free descent of the water meniscus (delivery under gravity) from the highest graduation line: to the lowest graduation line, in the case of Type 1 graduated pipettes; to the point at which the meniscus appears to come to rest in the jet, in the case of Type 2, Type 3 and Type 4 gr
48、aduated pipettes. For testing of the delivery time, the clean graduated pipette shall be held in a vertical position, if appropriate with no porous, air-permeable plug fitted, and filled with water to a few millimetres above the uppermost graduation line; the falling meniscus shall then be set to th
49、is line. Any drop adhering to the jet of the pipette shall be removed by touching the tip of the jet against the inner surface of an inclined glass vessel. Delivery shall then be made into another glass vessel (the receiving vessel), so that the tip of the jet is in contact with the inner surface of this receiving vessel, above the level of any collected liquid, but without movement of one against the other throughout the delivery period. IMPORTANT It is important that a glass receiving vessel is used.
