1、DB61 if * il DB611T 409-2007 2007-09-031b 2007-09-03nm DB61rr 409-2007 *;fiffft iJ IlfBA:J.J 4ttIlfHk 0 *fft$AWAMW*Po *fft.: $AOOWAo *iffftA: :r*, $f*f, Il*a, KffWo *fiffft*1Hx!t1ti 0 DB61ff 409-2007 1 $i! :$:,j:iJ; fi ffll j Y !WmjtfWj$IJ rPn 9=JFilWttL?;ltB7J1;L :$: *;f; fi mr tfWi$U Ml 9=J if!4lW
2、ttL*jj:;l j 0 2tJ.tHM1s1m Jt1tf F)iIJxftq f8iii1ii:$:fiJ;fttfJ 31 mrm JX;I;J :$:,fiFfitfJJ:o fL:MJt El JJ f8 i31 Jf.l X11j:, ;ltMiJFiJifilJi8 fe.k;qi (:f1H6llJji9: V-J ) l1J;fln:i)jmJ+l T:$:f;f;i, rm, !Z!EJJtlH,:$:f;j;fti6:r. X fij: tfJ iilI#f :$:0 fL M:Ifff BWJ tfJ i31 H:l X f!i: , ;ItiiifJi:$:jjjJ
3、Wf : . H71+: iIfi:tj FAD I.J)J(?:R -1i j J:,gIllif.;j:f,R,v,-iI!ii! NR lf.j 11t1.rVLHllf U f!iij 1 00 iJ; ill. , :fr 25C f8 f;J; fi 14 . F TI *rp 1U tfJ .J,:liit NADPHtfJj.jtf340nm1I: tfJ Ul1YtJJt 1J1:( 0 5 iltfflJ 5. 1 tiHP;rz:tK, JL1 10 j.l rn 0 6. 5 fJlJI),tJl 6. 6 iH:l ;ft, ftHJ!j:,:y=*, .JllZ;
4、W1;f0:Y1j7 7. 1 ilt1f I J!ciM 7. 1. 1 !i;IPRf ;ffi. 0.68g Q;t(mT-80ml rp, m HCI ifflrl1 pH 7.8, J:l:jm:*JEm3S: 100mL 7. 1. 2 NADP I1t;-t(iPf(fl O.5mgNADP, tj:mLfll O.Olmg FADo t!P); NADP yWo 7.1. 3 fIiElinRlfihd: m0.8mL 4U #fjMJmlt, Plg#fjMiliU!lH;i4i?Jo 7. 1. 4 He I: 2 mo I lLo 7.1.5 NaOH: 2moi/Lo
5、7.1.6 KOH: 2moi/Lo 7. 1. 7 Carrez ;Rf 7. 1.8 Carrez- I i 85mmolIL Carrez- I m: Jtx 3.60g I(4Fe (CN) 6 3H 2 0, 1.mA 100mL *.I./.mlf.c 7. 1. 8. 1 Carrez- II m 250mmoll LCarrez- II m: l$l1X 7. 20g Z,S01 7thO , :t.JI1A lOOmL *mmo 11fii1fJHB1, A Carrez- I mfll Carrez- II -m, Jt?tM6.JiJl Carrez mm.o 7. 2
6、ilt*$iMi 7.2. 1 i* ltt2rnL (g) fMW, 1JnA98rnLm:JJ(, m!;(JnJEJjt.j107Hrf, rt;7J.Et6-J, 7. 2. 2 1tiIi* l1X 5g ifi, :I1J JffiilxI/:Inl i*nrTn fTfrp, 11I1A 50 mL l:ffi.I./., :(r;J(mrp1if lSmin JFi , IttUHtW (20-2SC), hilA 6 mL Carrez ff.m., mNaOH iJ6l pH 8, ttw:*birp, mM*JE ?f.l-J 100mL. M;J, IMfDOmin,
7、i1IE: (;j:ij.iTh)LmLJf:L), J:l:jr),rY!o 7. 3 *1t:jIJ (t 1ilHT) 7.3. 1 i.is1 lttbtBilll, 11XiXEI1A l.OOOmLNADP r:1t1X, O.lOOmLNAD 1:1t:, 2.000 mL JJ(, tttJlI 3min lV)*7tlttll.EfX P llYtlf4PJ, fY1hlttJxrv.Jcnli: (A 40min), i.1;ltZ!l,Z ) DB61/T 409-2007 7tll-ffi. (A 2 ), tfMIJ1IXi11Bt *.MeIJkT 0.100, :
8、1:J illlUHiJfJ tii:W: 0 *1 #lJljff $ e I ff.n NADPlft 1. OOOmL I 1. OOOmL f+. - O. 100mL :iJ* 2.000mL j 1. 900mL itE:fIJ , RiWIJpA -F9uJ.OO: i ffl11ib:EJiJtlUHf 0.050mL I fUIl, ;tq;lIXflf fn;flJ e iJ1lllXll.i:.; (2) it.tI9: VjXMW c-X LI AX .(2) XdXV 2 X 1000 9 NJf1ib:i:it1t1Jit1J r- 05: 3. 050 X 62.
9、 0 c=- XL1AX f X 1. OOXO. 100X1000 1. 891 -XL1AX f iter: c PW!ii1)gH8j:R,1*lR, Jlf!.1iO!f: mL El3 lif-fftljiJff Ml Rq,l, W:fR, Jlf!.f:;7:;!lt: mL f-fMHlflJ#ff!ff1tr !:tBlillJt;fitL !fl.:;7:fi: em NADPH flJr:1t*: fr 340 nm 6.3 I x mmor l x eml 7tjm#AIWM1/tm:X ! 10.2 Mfi.tJINJ+J 1 I l 3 I DB61/T 409-2
10、007 10.2. 1 :bOivtH:mH5iIjij!JHtl:itt1ifE-j:frij!nE.g.H5it:p, f1;t:p AmJj( L OOOrnL - 2.000mL l.OOOmL O. lOOmL 1.900rnL 1.000mL O. 100mL 1.900mL 1.000mL 0.050mL O.050rnL 1.9)OmL *n, fi1lrJ!#dt0 w;Itt.jttmAl Hf1 7. 3;frul1f *11 :rU;fr?!J1UW. 2 X .1 A I!J+ tJIff- .1 A rr,J X 100%. . .(3) .1 A fME 4 DB61ff 409-2007 1li1 :1R A (i!i *!/.ti 1li1:1R ) 11:f:$lgr 1.M.mWtt, mM*. 2. 1ttlf.iq-l. :iflj*f;f)lq,JlIZ-ff: r 2C8C j!jcffiuJiX, NADP _t: 1t. Jtj.IDlItIJ:,d- .4 !WtE 2aC8CDJJ:!tJ3o i5(XijfjflJ:lifllmJlj!jcffiIJo I 3.q-lmm. II I i I I i 5 l I ! f
