SC T 7022-2020 对虾体内的病毒扩增和保存方法.pdf

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SC/T 7022 一 2020 对虾体内的病毒扩增和保存方法 1 范围 标准给IL了对虾体 l 人l(JJ耐高扩增平保存的缩Ill各 f l 、试剂和l材料 、 r .异, U 平II设备,规定了川虾体内的州 市扩增trl保存的操作在骤。 本标准适用于x,JYfF体 坷的协,: 年扩增平II,(, 飞 t=. 2 规范性引用文件 3 4 试剂和材料 4. 2 1 、 N缓冲液:见附录A r:j:i(I、 5 器材和设备 5. 1 匀浆r.tL 5. 2 高j冷的离心机。 5. 3 超速冷冻离心机。 5. 4 电子显微镜。 6 操作步骤 6. 1 实验动物选择 6. 1. 1 选界养成m阶段的 SPF凡纳院xJ 虾( Litenaeus vannmei)或其他品和11 SPF x,l虾,暂养7 d。 6. l. 2 随机挑取对虾个体,按 GB 丁28630. J、GB丁 28630. 2、GB/T 28630. :, GB丁 28630. 4, G 13丁

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