1、书书书中华人民共和国出入境检验检疫行业标准犛犖犜进出口蜂王浆中硝基呋喃类代谢物残留量的测定液相色谱质谱质谱法犇犲狋犲狉犿犻狀犪狋犻狅狀狅犳狀犻狋狉狅犳狌狉犪狀犿犲狋犪犫狅犾犻狋犲狊狉犲狊犻犱狌犲狊犻狀狉狅狔犪犾犼犲犾犾狔犳狅狉犻犿狆狅狉狋犪狀犱犲狓狆狅狉狋犎犘犔犆犕犛犕犛发布实施中华人民共和国国家质量监督检验检疫总局发布书书书前G21G21言G21G21本标准的附录G21G21附录G22为资料性附录G22本标准由国家认证认可监督管理委员会提出并归口G22本标准起草单位G23中华人民共和国浙江出入境检验检疫局G22本标准主要起草人G23陈笑梅G21刘海山G21谢文G21单慧君G21俞春燕G21杨
2、磊G21钱艳G21吴娟G22本标准系首次发布的出入境检验检疫行业标准G22G21G21G22G21G23G21G22G23G24G22G21G22G22G进出口蜂王浆中硝基呋喃类代谢物残留量的测定液相色谱G24质谱G21质谱法G24G21范围本标准规定了蜂王浆中呋喃唑酮代谢物G23G24氨基G24G25G24嗯唑烷酮G24G23G24G21G26G27G28G29G24G29G2AG2BG2CG29G2DG27G2EG27G28G24G25G24G29G28G2FG25G21G30G31G26G21呋喃它酮代谢物G23G24氨基G24G32G24吗啉代甲基G24G25G24嗯唑烷酮G24G23
3、G24G21G26G27G28G29G24G32G24G26G29G33G34G35G29G2DG27G28G29G26G2FG36G35G37G2DG24G29G2AG2BG2CG29G2DG27G2EG27G28G24G25G24G29G28G2FG25G21G38G30G31G26G21呋喃西林代谢物氨基脲G24G39G2FG26G27G3AG2BG33G3BG2BG2CG27G2EG2FG25G3CG3DG38G26和呋喃妥因代谢物G3EG24氨基乙内酰脲G24G3EG24G2BG26G27G28G29G35G37G24G2EG2BG28G36G29G27G28G25G21G3FG40
4、G26质谱G27质谱测定方法G22本标准适用于蜂王浆中G21G30G31G21G21G38G30G31G21G3CG3DG38和G21G3FG40残留的定性确证和定量测定G22G21G21方法提要样品经稀盐酸水解蛋白结合态中的硝基呋喃代谢物G25用G25G24硝基苯甲醛G24G25G24G41G22G21G26衍生化G25三氯乙酸沉淀去除蛋白质G25调G34G3F值G42G43G32后G25清液过G3FG44G22小柱净化G25分析物采用液相色谱G24串联质谱测定G25内标法定量G22G26G21试剂和材料除另有规定外G25试剂均为分析纯G25水为超纯水G22G26G43G24G21甲醇G23
5、色谱纯G22G26G43G21G21乙腈G23色谱纯G22G26G43G26G21乙酸乙酯G23色谱纯G22G26G43G27G21邻硝基苯甲醛G24G25G24G41G22G21G26G22G26G43G28G21二甲亚砜G23色谱纯G22G26G43G23G21乙酸铵G23色谱纯G22G26G43G29G21G32G26G26G29G2DG27G44乙酸铵水溶液G23称取G45G43G3EG46G47乙酸铵溶于适量水中G25再定容到G32G45G45G26G44G22G26G43G25G21G32G45G48三氯乙酸G23称G32G45G47三氯乙酸G25加G32G45G47水溶解G22G
6、26G43G2AG21G3EG26G29G2DG27G44氢氧化钠溶液G23称取G49G45G47氢氧化钠G25用水溶解定容至G3EG45G45G45G26G44G22G26G43G24G22G21G25G24G41G22G21衍生剂G24G32G45G26G26G29G2DG27G44G26G23称取G45G43G45G23G42G4AG47邻硝基苯甲醛到烧杯中G25加入G32G26G44二甲亚砜溶解G25该溶液的浓度为G32G45G26G26G29G2DG27G44G25现配G22G26G43G24G24G21G45G43G3EG26G29G2DG27G44磷酸氢二钾G23称取G4AG43
7、G42G3EG47无水磷酸氢二钾G25加入G32G45G45G26G44水溶解G22G26G43G24G21G21G45G43G25G26G29G2DG27G44盐酸溶液G23量取G3EG42G26G44浓盐酸用水定容至G3EG45G45G45G26G44G22G26G43G24G26G21G45G43G25G48乙酸G24乙腈G24G42G4BG23G25体积比G26G23G45G43G25G48乙酸G42G45G26G44与G23G45G26G44乙腈混匀G22G26G43G24G27G21标准溶液配制G26G43G24G27G43G24G21硝基呋喃类代谢物标准品G23G21G30G31
8、G21G3CG3DG38G28G3FG4CG2DG21G21G3FG40G28G3FG4CG2DG21G21G38G30G31纯度均大于G46G46G48G22G26G43G24G27G43G21G21硝基呋喃类代谢物同位素标准品G23G21G30G31G24G40G49纯度大于G46G4AG48G25G3CG4CG21G24G3EG32G41G25G24G3EG23G4CG28G3FG4CG2D纯度大于G46G46G48G25G21G3FG40G24G3EG23G4CG23纯度大于G46G4AG48G25G21G38G30G31G24G40G32纯度大于G46G4AG48G22G26G43G
9、24G27G43G26G21标准储备液G23分别称取适量G21G30G31G21G21G38G30G31G21G3CG3DG38G28G3FG4CG2D和G21G3FG40G28G3FG4CG2D硝基呋喃代谢物标准G25用甲醇溶解并定容G3EG43G45G26G47G27G26G44G25G45G4DG22G49G4D保存G25有效期G4E个月G22G26G43G24G27G43G27G21混合标准溶液G23分别准确移取适量G3EG43G45G26G47G27G26G44G21G30G31G21G3CG3DG38G21G21G3FG40G21G21G38G30G31标准储备液G24G23G43
10、G3EG4EG26G25G3EG21G22G21G23G21G22G23G24G22G21G22G22G用甲醇稀释定容至G3EG45G28G47G27G26G44G25G45G4DG22G49G4D保存G25有效期G42G2EG22G26G43G24G27G43G28G21同位素内标储备液G23分别称取适量G21G30G31G24G40G49G21G21G3FG40G24G3EG23G4CG23G21G21G38G30G31G24G40G32和G3CG4CG21G24G3EG32G41G25G24G3EG23G4CG28G3FG4CG2D硝基呋喃代谢物同位素标准品G25用甲醇溶解并定容到G25
11、G32G26G44棕色容量瓶中G25该溶液浓度为G3EG43G45G26G47G27G26G44G25G45G4DG22G49G4D保存G22G26G43G24G27G43G23G21混合同位素内标溶液G23分别准确移取适量G3EG26G47G27G26G44G21G30G31G24G40G49G21G3CG4CG21G24G3EG32G41G25G24G3EG23G4CG21G21G3FG40G24G3EG23G4CG23G21G21G38G30G31G24G40G32储备液G24G23G43G3EG49G43G23G26G25用甲醇稀释定容为G3EG45G28G47G27G26G44G25
12、G45G4DG22G49G4D保存G22G26G43G24G27G43G29G21混合标准工作溶液G23分别取混合标准溶液G24G23G43G3EG49G43G49G26G45G23G44G25G4EG45G23G44G25G3EG45G45G23G44G25G25G45G45G23G44G25G49G45G45G23G44G25G3EG45G45G45G23G44G22加入混合同位素内标溶液G24G23G43G3EG49G43G4EG26G25G45G45G23G44G25G23G26G44G45G43G25G26G29G2DG27G44稀盐酸和G3EG45G45G23G44G25G24G4
13、1G22G21衍生剂G25混匀后G23G42G4D放置过夜G22用G3EG26G29G2DG27G44氢氧化钠溶液调节G34G3FG42G43G32G4FG45G43G25G25G32G26G44乙酸乙酯液液萃取G25将有机相在G49G45G4D氮吹至干后G25用G3EG43G45G26G44G45G43G25G48乙酸G24乙腈G24G42G4BG23G25体积比G26G24G23G43G3EG23G26溶解残渣G25该混合标准工作溶液相当于样品中含有G45G23G47G27G50G47G25G45G43G23G23G47G27G50G47G25G45G43G32G23G47G27G50G4
14、7G25G3EG43G45G23G47G27G50G47G25G25G43G45G23G47G27G50G47G25G32G43G45G23G47G27G50G47的硝基呋喃代谢物残留G22G26G43G24G28G21G3FG44G22小柱G23G30G21G3CG51G3CG3FG44G22小柱G25G4EG45G26G47G25G23G26G44或相当者G22使用前用G32G26G44甲醇和G32G26G44水预淋洗G22G27G21仪器和设备G27G43G24G21液相色谱G24质谱G27质谱联用仪G23配有电喷雾源G24G3DG3CG51G26G22G27G43G21G21旋转浓缩器
15、G22G27G43G26G21混匀器G22G27G43G27G21真空固相萃取装置G22G27G43G28G21G34G3F计G23测量精度G4FG45G43G45G25G22G27G43G23G21浓缩瓶G23G3EG45G45G26G44G22G27G43G29G21具盖塑料离心管G23G32G45G26G44G22G27G43G25G21离心机G23G49G45G45G45G33G27G26G27G28G22G27G43G2AG21烘箱G22G28G21试样制备与保存取G32G45G45G47代表性蜂王浆样品G25在室温下解冻G25等样品全部融化后搅匀G25将试样均分成两份G25分别装入
16、样品瓶中G25密封G25并标明标记G22一份作为试验样G25另一份在G52G3EG4AG4D保存G22在制样的操作过程中G25应防止样品污染或发生残留物含量的变化G22G23G21测定步骤G23G25G24G21提取和净化称取混匀的蜂王浆样品G25G47G24精确至G45G43G45G3EG47G26于G32G45G26G44具盖塑料离心管中G25分别加入G25G45G45G23G44混合同位素内标溶液G24G23G43G3EG49G43G4EG26G25G25G32G26G44G45G43G25G26G29G2DG27G44盐酸溶液和G3EG45G45G23G44G25G24G41G22G2
17、1衍生剂G24G23G43G3EG45G26G25混匀后放入G23G42G4DG4FG25G4D烘箱内G25避光放置过夜G22加G3EG43G45G26G44G32G45G48三氯乙酸G25轻轻混匀后G25G49G45G45G45G33G27G26G27G28离心G32G26G27G28G25将上清液过滤G22滤液用G3EG26G29G2DG27G44氢氧化钠溶液调节G34G3FG42G43G32G4FG45G43G25G25过经预淋洗的G3FG44G22小柱G24G23G43G3EG32G26G25再加G3EG45G26G44水清洗G3FG44G22小柱G25用G3EG45G26G44乙酸乙
18、酯洗脱G25收集洗脱液G25G49G45G4D以下真空浓缩至干后G25用G3EG43G45G26G44G45G43G25G48乙酸G24乙腈G24G42G4BG23G25体积比G26G24G23G43G3EG23G26溶解残渣G25过G45G43G49G32G23G26的滤膜G25滤液供液相色谱串联质谱测定G22G23G25G21G21测定G23G25G21G25G24G21液相色谱串联质谱条件G2BG26G21色谱柱G23G4CG4AG3EG32G45G26G26G53G32G26G26G25G32G23G26或相当者G29G3BG26G21流动相梯度洗脱条件见表G3EG29G3AG26G2
19、1流速G23G45G45G23G44G27G26G27G28G29G25G21G22G21G23G21G22G23G24G22G21G22G22GG2EG26G21进样量G23G23G45G23G44G29G21G21G2FG26G21离子源G23电喷雾离子源G29G54G26G21扫描方式G23正离子扫描G29G47G26G21检测方式G23多反应监测G24G38G55G38G26G29G35G26G21分辨率G23单位分辨G29G27G26G21其他参考条件参见附录G21G22表G24G21梯度洗脱条件时间G27G26G27G28乙腈G27G48 G32G26G26G29G2DG27G44
20、醋酸铵水溶液G27G48G45G43G45 G23G45 G42G45G42 G46G45 G3EG45G3EG25 G46G45 G3EG45G3EG25G43G3E G23G45 G42G45G3EG42 G23G45 G42G45G23G25G21G25G21G21液相色谱G21串联质谱测定根据试样中被测样液的含量情况G25选取响应值相近的标准工作液进行色谱分析G22标准工作液和样液中待测物的响应值均应在校正曲线线性响应范围内G25如果超出G25应稀释G22在上述色谱条件下G3CG3DG38G21G21G3FG40G21G21G38G30G31和G21G31G30的参考保留时间分别约为G
21、46G43G4AG26G27G28G21G3EG45G43G3EG26G27G28G21G3EG45G43G42G26G27G28和G3EG3EG43G25G26G27G28G25四种硝基呋喃代谢物标准品多反应监测G24G38G55G38G26色谱图参见附录G22中图G22G43G3EG22G23G25G21G25G26G21定性测定按照上述条件测定样品和标准工作溶液G25如果样品的质量色谱峰保留时间与标准品一致G25允许偏差小于G4FG25G43G32G48G29定性离子对的相对丰度与浓度相当的标准工作溶液的相对丰度一致G25相对丰度允许偏差不超过表G25规定的范围G25则可判断样品中存在相
22、应的被测物G22表G21G21定性确证时相对离子丰度的最大允许偏差相对离子丰度G27G48 G22G32G45 G23G32G45G22G25G45 G23G25G45G22G3EG45 G24G3EG45允许的相对偏差G27G48 G4FG25G45 G4FG25G32 G4FG23G45 G4FG32G45G23G25G21G25G27G21定量测定按照内标法进行定量计算G22G23G25G21G25G28G21空白试验除不加试样外G25均按上述操作步骤进行G22G29G21结果计算和表述用色谱数据处理机或用式G24G3EG26计算试样中硝基呋喃代谢物残留含量G23G21G22G23G24
23、G45G25G26G27G25G23G27G39G25G26G45G25G28G26G24G45G25G23G45G25G26G27G39G25G29G27G25G3EG45G45G48G2AG2AG2AG2AG2AG2AG2AG2AG2AG2AG24G3EG26G21G21式中G23G21G2BG2BG2B试样中被测组分的残留量G29G23G56G45G2BG2BG2B样品溶液中内标物的浓度G29G26G27G2BG2BG2B样品溶液中待测组分的峰面积G29G26G56G45G2BG2BG2B样品溶液中内标物峰面积G29G23G27G39G2BG2BG2B标准溶液的浓度G29G23G45G2
24、BG2BG2B标准溶液中内标物的浓度G29G26G27G39G2BG2BG2B标准溶液峰面积G29G26G45G2BG2BG2B标准溶液中内标物峰面积G29G23G21G22G21G23G21G22G23G24G22G21G22G22GG28G2BG2BG2B样品溶液最终定容的体积G29G29G27G2BG2BG2B样品溶液所代表的试样质量G25单位为克G24G47G26G22G25G21测定低限G21回收率G25G25G24G21测定低限硝基呋喃类代谢物残留测定低限均为G45G43G32G23G47G27G50G47G22G25G25G21G21回收率在蜂王浆样品中添加硝基呋喃类代谢物浓度在
25、G45G43G32G23G47G27G50G47G21G3EG43G45G23G47G27G50G47G21G25G43G45G23G47G27G50G47时G25回收率见表G23G22表G26G21蜂王浆中硝基呋喃类代谢物测定回收率范围添加浓度G45G43G32G23G47G27G50G47 G3EG43G45G23G47G27G50G47 G25G43G45G23G47G27G50G47G3CG3DG38 G4AG42G43G4EG48G22G3EG45G49G43G45G48 G4AG46G43G3EG48G22G3EG45G4EG43G45G48 G46G3EG43G45G48G22G
26、3EG45G4EG43G45G48G21G3FG40 G4AG42G43G25G48G22G3EG45G45G43G49G48 G4AG42G43G4EG48G22G3EG45G25G43G45G48 G4AG49G43G45G48G22G46G42G43G45G48G21G38G30G31 G46G3EG43G4EG48G22G3EG45G23G43G25G48 G46G45G43G42G48G22G3EG45G49G43G45G48 G4AG42G43G32G48G22G3EG45G49G43G45G48G21G30G31 G46G3EG43G4EG48G22G3EG45G49G43G25
27、G48 G4AG46G43G4EG48G22G3EG45G32G43G45G48 G4AG4AG43G45G48G22G3EG45G3EG43G45G48G49G21G22G21G23G21G22G23G24G22G21G22G22G附G21录G21G26G24资料性附录G26G26G27G28G27G22G22G22G29G2AG24G2BG21G21G2BG21系统电喷雾离子源参考条件G24G26G21G21监测离子对及电压G21气压参数G23G2BG26G21气帘气压力G24G4CG57G55G26G23G3EG42G25G43G23G42G32G50G58G2BG24G25G32G58
28、G39G27G26G29G3BG26G21雾化气压力G24G59G3CG3EG26G23G25G4AG46G43G32G46G50G58G2BG24G49G25G58G39G27G26G29G3AG26G21辅助气压力G24G59G3CG25G26G23G23G3EG45G43G25G42G32G50G58G2BG24G49G32G58G39G27G26G29G2EG26G21电喷雾电压G24G51G3CG26G23G49G4AG45G45G5AG29G2FG26G21离子源温度G24G5BG3DG38G26G5BG3DG38G23G32G49G45G4DG29G54G26G21碰撞气压力G2
29、4G4CG21G40G26G23G23G49G43G49G42G32G50G58G2BG24G32G58G39G27G26G29G47G26G21G3DG58电压G23G46G5AG29G35G26G21离子对G21G40G58G21G4CG3D和G4CG5CG58见表G21G43G3EG22表G26G43G24G21离子对G21G2CG27G21G2AG2D和G2AG2EG27名G21G21称定性离子对G26G27G2C保留时间G27G26G27G28去簇电压G24G40G58G26G27G5A碰撞气能量G24G4CG3DG26G27G5A碰撞室出口电压G24G4CG5CG58G26G27G
30、5AG3CG3DG38G24G41G22G21G25G45G46G43G25G27G3EG4EG4EG43G25G2BG46G43G4A G4EG45 G3EG32 G3EG3EG25G45G46G43G25G27G3EG46G25G43G25 G46G43G4A G4EG45 G3EG42 G3EG3EG3CG4CG21G24G3EG32G41G25G24G3EG23G4CG24G41G22G21 G25G3EG25G43G25G27G3EG4EG4AG43G25 G46G43G4A G4EG45 G3EG32 G3EG3EG21G3FG40G24G41G22G21G25G49G46G43G
31、25G27G3EG23G49G43G3EG2BG3EG45G43G3E G4EG4E G3EG4A G3EG3EG25G49G46G43G25G27G3EG45G49G43G3E G3EG45G43G3E G4EG4E G23G25 G3EG3EG21G3FG40G24G3EG23G4CG23G24G41G22G21 G25G32G25G43G25G27G3EG23G49G43G25 G3EG45G43G3E G4EG4E G3EG4A G3EG3EG21G38G30G31G24G41G22G21G23G23G32G43G25G27G25G4EG25G43G23G2BG3EG45G43G42
32、G4EG4E G25G32 G3EG4AG23G23G32G43G25G27G25G46G3EG43G23 G3EG45G43G42 G4EG4E G3EG42G43G42 G3EG4AG21G38G30G31G24G40G32G24G41G22G21 G23G49G45G43G23G27G25G46G4EG43G49 G3EG45G43G42 G4EG4E G3EG4A G3EG3EG21G30G31G24G41G22G21G25G23G4EG43G25G27G3EG23G49G43G25G2BG3EG3EG43G25 G4EG4E G3EG4AG43G23 G3EG25G25G23G4EG
33、43G25G27G3EG45G49G43G3E G3EG3EG43G25 G4EG4E G23G25 G3EG4AG21G30G31G24G40G49G24G41G22G21 G25G49G45G43G25G27G3EG23G49G43G25 G3EG3EG43G25 G4EG4E G3EG4A G3EG3EG2B为定量离子对G22G3EG26G21非商业性声明G23附录G21所列参数是在G21G58G51G49G45G45G45质谱仪完成的G25此处列出试验用仪器型号仅是为了提供参考G25并不涉及商业目的G25鼓励标准使用者尝试不同厂家和型号的仪器G22G32G21G22G21G23G21G
34、22G23G24G22G21G22G22G附G21录G21G2FG24资料性附录G26硝基呋喃类代谢物标准品多反应监测G24G2BG30G2BG26色谱图图G2FG43G24G21硝基呋喃类代谢物标准品G24G21G25G22G21G31G21G32G31G26和同位素内标G24G24G25G22G21G31G21G32G31G26多反应监测G24G2BG30G2BG26色谱图G4EG21G22G21G23G21G22G23G24G22G21G22G22GG21G22G23G24G25G22G23G26G27G28G28G24G29G27G21G2AG28G28G24G29G2BG22G2CG
35、2DG2EG2FG30G30G2DG2AG28G26G2AG23G26G2AG23G24G31G22G2DG2EG2FG28G2CG22G23G32G2AG2DG2FG33G24G2AG28G28G24G29G24G30G34G35G2EG2FG30G30G2DG2AG28G26G2AG23G26G2FG30G36G23G22G36G22G30G24G26G31G37G2AG28G26G2FG30G38G28G26G24G23G2DG2EG24G39G2EG2AG23G3AG24G22G2CG3BG24G23G2DG2FG2CG2FG39G2AG2DG2FG22G28G2AG28G26G27G
36、39G39G23G24G26G2FG2DG2AG2DG2FG22G28G27G26G32G2FG28G2FG30G2DG23G2AG3CG2DG2FG22G28G22G2CG2DG2EG24G3DG24G22G36G3EG24G2CG30G3FG24G36G38G31G3EG2FG39G22G2CG3BG2EG2FG28G2AG43G35G2EG2FG30G30G2DG2AG28G26G2AG23G26G2FG30G26G23G2AG2CG2DG24G26G31G37G40G2EG24G41G2FG2AG28G3AG42G28G2DG23G37G3CG42G29G2FG2DG43G28G30G
37、36G24G39G2DG2FG22G28G2AG28G26G44G38G2AG23G2AG28G2DG2FG28G24G2BG38G23G24G2AG38G22G2CG2DG2EG24G3DG24G22G36G3EG24G2CG30G3FG24G3CG36G38G31G3EG2FG39G22G2CG3BG2EG2FG28G2AG43G35G2EG24G32G2AG2FG28G26G23G2AG2CG2DG24G23G30G22G2CG2DG2EG2FG30G30G2DG2AG28G26G2AG23G26G2AG23G24G3BG2EG24G28G45G2FG2AG22G32G24G2FG25G
38、46G2FG38G47G2AG2FG30G2EG2AG28G25G45G2FG24G48G24G28G25G49G2EG2AG28G47G38G2FG41G38G28G25G4AG38G3BG2EG38G28G3CG37G2AG28G25G4AG2AG28G3AG46G24G2FG25G44G2FG2AG28G4AG2AG28G25G48G38G4BG38G2AG28G43G35G2EG2FG30G30G2DG2AG28G26G2AG23G26G2FG30G2AG36G23G22G2CG24G30G30G2FG22G28G2AG3EG30G2DG2AG28G26G2AG23G26G2CG22G
39、23G24G28G2DG23G37G3CG24G29G2FG2DG2FG28G30G36G24G39G2DG2FG22G28G2AG28G26G4CG38G2AG23G2AG28G2DG2FG28G24G36G23G22G32G38G3EG3AG2AG2DG24G26G2CG22G23G2DG2EG24G2CG2FG23G30G2DG2DG2FG32G24G43G4DG22G2DG24G23G35G2EG2FG30G42G28G3AG3EG2FG30G2EG4EG24G23G30G2FG22G28G25G2AG2DG23G2AG28G30G3EG2AG2DG2FG22G28G2CG23G22G
40、32G2DG2EG24G3BG2EG2FG28G24G30G24G2DG24G29G2DG25G2FG30G30G22G3EG24G3EG37G2CG22G23G3AG38G2FG26G2AG28G39G24G43G42G21G22G21G23G21G22G23G24G22G21G22G22GG4FG24G2DG24G23G32G2FG28G2AG2DG2FG22G28G22G2CG28G2FG2DG23G22G2CG38G23G2AG28G32G24G2DG2AG31G22G3EG2FG2DG24G30G23G24G30G2FG26G38G24G30G2FG28G23G22G37G2AG3E
41、G41G24G3EG3EG37G2CG22G23G2FG32G36G22G23G2DG2AG28G26G24G29G36G22G23G2DG2BG47G3DG46G3BG3CG50G49G27G50G49G51G21G49G39G22G36G24G35G2EG2FG30G30G2DG2AG28G26G2AG23G26G30G36G24G39G2FG2CG2FG24G30G2DG2EG24G32G24G2DG2EG22G26G22G2CG26G24G2DG24G23G32G2FG28G2AG2DG2FG22G28G22G2CG2CG38G23G2AG52G22G3EG2FG26G22G28G24
42、G32G24G2DG2AG31G22G3EG2FG2DG24G53G3CG27G32G2FG28G22G3CG22G29G2AG52G22G3EG2FG26G2FG28G3CG54G3CG22G28G24G24G27G55G40G26G25G2CG38G23G2AG3EG2DG2AG26G22G28G24G32G24G2DG2AG31G22G3EG2FG2DG24G53G3CG27G32G2FG28G22G3CG56G3CG32G22G23G36G2EG22G3EG2FG28G22G32G24G2DG2EG37G3EG3CG22G29G2AG52G22G3EG2FG26G2FG28G3CG54
43、G3CG22G28G24G24G27G50G55G40G26G25G28G2FG2DG23G22G3CG2CG38G23G2AG52G22G28G24G32G24G2DG2AG31G22G3EG2FG2DG24G30G24G32G2FG39G2AG23G31G2AG52G2FG26G24G24G49G42G50G26G25G2AG28G26G28G2FG2DG23G22G2CG38G23G2AG28G2DG22G2FG28G32G24G2DG2AG31G22G3EG2FG2DG24G51G3CG2AG32G2FG28G22G2EG37G26G2AG28G2DG22G2FG28G24G27G47
44、G4FG26G23G24G30G3CG2FG26G38G24G30G2FG28G23G22G37G2AG3EG41G24G3EG3EG37G31G37G2EG2FG3AG2EG3CG36G24G23G2CG22G23G32G2AG28G39G24G3EG2FG4CG38G2FG26G39G2EG23G22G32G2AG2DG22G3AG23G2AG36G2EG37G3CG2DG2AG28G26G24G32G32G2AG30G30G30G36G24G39G2DG23G22G32G24G2DG23G37G43G35G2EG2FG30G30G2DG2AG28G26G2AG23G26G2FG30G2A
45、G36G36G3EG2FG39G2AG31G3EG24G2DG22G2DG2EG24G4CG38G2AG28G2DG2FG2CG37G2AG28G26G39G22G28G2CG2FG23G32G22G2CG27G55G40G25G27G50G55G40G25G27G47G4FG25G49G42G50G23G24G30G2FG26G38G24G30G2FG28G23G22G37G2AG3EG41G24G3EG3EG37G43G54G21G3DG23G2FG28G39G2FG36G3EG24G3DG23G22G2DG24G2FG28G2FG28G30G2AG32G36G3EG24G28G24G24
46、G26G2DG22G31G24G36G23G24G39G2FG36G2FG2DG2AG2DG24G26G31G37G2DG23G2FG39G2EG3EG22G23G22G2AG39G24G2DG2FG39G2AG39G2FG26G30G22G3EG38G2DG2FG22G28G2AG2CG2DG24G23G39G22G32G31G2FG28G24G26G2EG37G26G23G22G3EG37G30G2FG30G22G2CG2DG2EG24G36G23G22G2DG24G2FG28G3CG31G22G38G28G26G28G2FG2DG23G22G2CG38G23G2AG28G32G24G2D
47、G2AG31G22G3EG2FG2DG24G30G2AG28G26G26G24G23G2FG33G2AG2DG2FG30G2AG2DG2FG22G28G22G2CG2DG2EG24G23G24G30G38G3EG2DG2FG28G3AG32G24G2DG2AG31G22G3EG2FG2DG24G30G25G2FG2DG2EG54G3CG28G2FG2DG23G22G3CG31G24G28G52G2AG3EG26G24G2EG37G26G24G24G4DG2BG27G26G26G38G23G2FG28G3AG2AG28G22G33G24G23G28G2FG3AG2EG2DG2FG28G39G38
48、G31G2AG2DG2FG22G28G43G27G26G41G38G30G2DG2DG2EG24G30G22G3EG38G2DG2FG22G28G2DG22G36G47G57G43G56G2AG28G26G39G3EG24G2AG28G38G36G31G37G47G46G2BG39G2AG23G2DG23G2FG26G3AG24G43G43G2DG25G2AG30G26G24G2DG24G23G32G2FG28G24G26G2AG28G26G39G22G28G2CG2FG23G32G24G26G31G37G2EG2FG3AG2EG3CG36G24G23G2CG22G23G32G2AG28G39
49、G24G3EG2FG4CG38G2FG26G39G2EG23G22G32G2AG2DG22G3AG23G2AG36G2EG37G3CG2DG2AG28G26G24G32G32G2AG30G30G30G36G24G39G2DG23G22G32G24G2DG23G37G43G43G28G2DG24G23G28G2AG3EG30G2DG2AG28G26G2AG23G26G32G24G2DG2EG22G26G25G2AG30G38G30G24G26G2DG22G4CG38G2AG28G2DG2FG2CG37G43G53G21G3FG24G2AG3AG24G28G2DG30G2AG28G26G32G2AG2DG24G23G2FG2AG3EG30G58G28G3EG24G30G30G30G36G24G39G2FG2CG2FG24G26G28G22G2DG24G30G25G2A
