1、Designation: C 1338 00Standard Test Method forDetermining Fungi Resistance of Insulation Materials andFacings1This standard is issued under the fixed designation C 1338; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of l
2、ast revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers the determination of the abilityof new insulation materials and their facings to support funga
3、lgrowth.1.2 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.1.3 The
4、 values stated in inch-pound units are to be regardedas the standard. The values given in parentheses are providedfor information only.2. Significance and Use2.1 The type of materials used in the manufacture ofinsulation products and the type of membrane used to facethese products can sometimes affe
5、ct fungi sustenance in thepresence of high humidity.2.2 This test method is used to determine the relative abilityof an insulation and its facing to support or resist fungal growthunder conditions favorable for their development.2.3 This test method uses a comparative material to deter-mine the rela
6、tive ability of a material to support fungal growth.In some specialized product areas, it is required that no growthtake place. In such cases, the use of the comparative materialis omitted and the pass/fail criterion is based upon growth.3. Apparatus3.1 GlasswareSterile disposable petri dishes, 4 or
7、 6 in.(100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size arepreferred. For larger specimens, trays of borosilicate glass orbaking dishes up to 16 by 20 in. (400 by 600 mm) in size maybe used.3.2 Environmental Chamber or CabinetEquipment forthis test method shall maintain a temperature of 82.4
8、to 86F(28 to 30C) and a relative humidity of 95 % (64 %).Provisions shall be made to prevent condensation from drip-ping on the test specimen. There shall be free circulation of airaround the test chamber.3.3 AtomizerA chromatography atomizer capable of pro-viding 100 000 6 20 000 spores/in.2(15 000
9、 6 3000 spores/cm2) shall be used for inoculation.3.4 Autoclavable Biohazard Bags, or metal pan able towithstand autoclaving.4. Reagents and Materials4.1 Purity of WaterUnless otherwise specified, referencesto water shall be understood to mean sterile distilled water orwater of equal purity.4.2 Inoc
10、ulum:Fungi ATCC2Aspergillus niger 9642Aspergillus versicolor 11 730Penicillium funiculosum 11 797Chaetomium globosum 6205Aspergillus flavus 96434.3 CulturesMaintain cultures of the Aspergillus fungiseparately on Czapek Dox agar (see Note 1). Culture theChaetomium globosum on strips of Whatman 500 fi
11、lter paperon the surface of Czapek Dox agar. Maintain the Penicilliumfungi on Sabouraud Dextrose agar. The stock cultures may bekept for not more than 4 months at 43 6 7F (6 6 4C) atwhich time subcultures shall be made, and new stocks selectedfrom the subcultures. Obtain new cultures from ATCC annu-
12、ally. Incubate subcultures used for preparing new stock cul-tures or the spore suspension at 86 6 4F (30 6 2C) for 5days or longer.NOTE 1This media is readily available from any science/microbiological supply house.5. Specimens5.1 Viability SpecimensDetermine the viability of thespore suspension dur
13、ing incubation with these controls: witheach daily group of tests, place one piece of sterilized What-man 500 filter paper, 1 in.2(6.4 cm2) on each of two preparedhardened Czapek Dox agar specimens in separate petri dishes.Prepare a third petri dish with Sabouraud Dextrose agar.5.2 Comparative Mater
14、ialA white birch tongue depres-sor, 0.75 by 6 in. (20 by 150 mm) or southern yellow pine is the1This test method is under the jurisdiction of ASTM Committee C016 onThermal Insulation and is the direct responsibility of Subcommittee C16.31 onChemical and Physical Properties.Current edition approved M
15、ay 10.2000. Published August 2000.2Available from American Type Culture Collection, 12301 Parklawn Drive,Rockville, MD 20852.1Copyright ASTM, 100 Barr Harbor Drive, West Conshohocken, PA 19428-2959, United Sparative item to determine the relative growth on speci-mens being tested. The choice of comp
16、arative item should benoted. Refer to the appropriate materials standard for relevantcomparative materials.NOTE 2The comparative item is chosen to reflect the buildingconstruction material. In some cases, other material may be more relevantas a comparative item.5.3 Test Specimens:5.3.1 Prepare tripl
17、icate specimens from each test sample.5.3.1.1 If the test sample is of different construction on eachface, prepare triplicate specimens of each face in the upposition.5.3.2 The preferred specimen thickness is 0.75 in. (20 mm).If altering the specimen thickness is required, ensure that thesurface to
18、be tested has not been altered and is in the upposition. If the normal specimen thickness is less than 0.75 in.(20 mm), then test at its normal thickness. If the specimencontainer will be covered, the specimen shall not make contactwith it.5.3.2.1 When testing loose materials such as blowing orpouri
19、ng insulations, refer to the appropriate materials standardfor sample preparation recommendations.5.3.3 It is sometimes desirable to test the adhesive used tobond a facing to the substrate. In such case only, peel back thefacing approximately half way across the face of the specimenbefore testing. N
20、ote this change clearly in the report.6. Procedure6.1 Spore SuspensionPrepare a spore suspension of eachof the five fungi by pouring into one subculture of each fungusa 10 mL portion of a sterile solution containing a sufficientquantity, not to exceed 0.10 g/L, of a nontoxic wetting agentsuch as sor
21、bitan monoleate (Tween-80), sodium dioctyl sulfo-succinate, or sodium lauryl sulfate to prevent clumping of thespores. Gently scrape the surface growth from the culture ofthe test organism using a sterile platinum or nichrome inocu-lating wire. Pour the spore charge into a sterile 125-mLglass-stoppe
22、red Erlenmeyer flask containing 45 6 1mLofsterile water, and 50 to 75 solid glass beads, approximately0.20 in. (5 mm) in diameter. Vigorously shake the flask toliberate the spores from the fruiting bodies and to break thespore clumps. Filter the dispersed fungal spore suspensionthrough at least a 0.
23、24-in. (6-mm) layer of glass wool containedin a glass funnel, into a sterile flask. This process is intended toremove large mycelial fragments and clumps of agar that couldinterfere with the spraying process. Centrifuge the filteredspore suspension. Remove all the supernatant down to thesurface of t
24、he spore pellet, taking care not to remove or disturbthe spore pellet. Resuspend the residue in 50 mL of sterilewater and centrifuge. (It may be necessary to add a smallquantity of nontoxic wetting agent, not to exceed 0.10 g/L, toprevent clumping of the spores.) Wash the spores obtainedfrom each of
25、 the fungi in this manner three times. Dilute thefinal washed residue with distilled water in such a manner thatthe resultant spore suspension shall contain 1 000 0006200 000 spores per mL as determined with a counting chamber.Repeat the operation for each organism used in the test andblend equal vo
26、lumes of the resultant spore suspensions toobtain the final mixed spore suspension. The spore suspensionmay be prepared fresh each day or may be held at 43 6 7F (66 4C) for not more than 28 days, or until the viability testindicates poor growth, or until growth appears in the sealedstorage bottle.6.
27、2 Inoculation of Test Specimens, Comparative Material,and Control SpecimensPrecondition the chamber and itscontents at 866 4F (30 6 2C) and 956 4 % relativehumidity for at least 4 h. Place each test, comparative material,and viability control specimen in separate sterile petri dishes.Inoculate each
28、specimen with approximately 0.50 mL of sporesuspension by spraying exposed surfaces in the form of a finemist from a previously sterilized atomizer or nebulizer. If aspecimen container is covered, it shall be loose fitting glass toallow air circulation. Place specimens in the chamber andimmediately
29、begin incubation.6.3 IncubationMaintain the test chamber at 86 6 4F (306 2C) and a relative humidity of 95 6 4 % throughout thetest. Keep the test chamber closed during incubation exceptduring inspection. After 3 to 7 days, inspect the controlspecimens. If control specimens do not show an abundance
30、ofgrowth at this time, repeat the entire test. If growth is presenton the control specimens, continue the test for a minimumperiod of 28 days 6 8 h from the time of incubation. Somematerials may require longer periods of incubation, therefore,the test period may be extended. Note all incubation time
31、s.Refer to the materials specifications for incubation periods.7. Interpretation of Results7.1 InspectionAt the end of the incubation period, removethe test specimens and comparative item from the test chamberand examine at 403 magnification.7.2 Interpretation of ResultsTest specimens that havegrowt
32、h greater than that on the comparative item shall beconsidered to have failed. Test specimens on which the growthis not greater than that on the comparative item shall beconsidered to have passed.7.2.1 If no growth is the criterion, the observation of anygrowth on the test specimen shall be consider
33、ed a failure.7.3 After completion of inspection, all test specimens andtest equipment shall be autoclaved under the manufacturersautoclave instructions to ensure destruction of vegetative cellsand spores to prevent accidental contamination to the labora-tory and environment.8. Report8.1 Report the f
34、ollowing information:8.1.1 Complete identification of the material tested,8.1.2 Identification of variable test conditions (comparativeitem, sample moistening, test duration) criterion used todetermine Pass/Fail (comparative item or no growth), and8.1.3 Results of the test.9. Precision and Bias9.1 N
35、o statement is made about either the precision or thebias of this fungi resistance test method since the test is asubjective, visual determination of whether the test materialdiffers from a comparative material.C 1338210. Keywords10.1 batts; blanket; boards; cellulose; facings; foam; fungiresistance
36、; insulation; loose-fill; mineral fiberThe American Society for Testing and Materials takes no position respecting the validity of any patent rights asserted in connectionwith any item mentioned in this standard. Users of this standard are expressly advised that determination of the validity of any
37、suchpatent rights, and the risk of infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are inv
38、ited either for revision of this standard or for additional standardsand should be addressed to ASTM Headquarters. Your comments will receive careful consideration at a meeting of the responsibletechnical committee, which you may attend. If you feel that your comments have not received a fair hearin
39、g you should make yourviews known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the above address or at610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website (www.astm.org).C 13383
