1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 4719:2012Essential oil of spike lavender (Lavandula latifolia Medikus), Spanish typeBS ISO 4719:2012 BRITISH STANDARDNational forewordThis British Standard is the UK imple
2、mentation of ISO 4719:2012. The UK participation in its preparation was entrusted to T e c h n i c a l Committee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary prov
3、isions of a contract. Users are responsible for its correct application. The British Standards Institution 2012. Published by BSI Standards Limited 2012.ISBN 978 0 580 73630 8 ICS 71.100.60 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was pub
4、lished under the authority of the Standards Policy and Strategy Committee on 31 January 2013.Amendments issued since publicationDate T e x t a f f e c t e dBS ISO 4719:2012 ISO 2012Essential oil of spike lavender (Lavandula latifolia Medikus), Spanish typeHuile essentielle daspic (Lavandula latifoli
5、a Medikus), type EspagneINTERNATIONAL STANDARDISO4719Third edition2012-12-15Reference numberISO 4719:2012(E)BS ISO 4719:2012ISO 4719:2012(E)ii ISO 2012 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2012All rights reserved. Unless otherwise specified, no part of this publication may be reproduc
6、ed or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1211 Geneva 20Tel. + 41 22 749 01
7、11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 4719:2012ISO 4719:2012(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standa
8、rds is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also tak
9、e part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is t
10、o prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.Attention is drawn to the possibility t
11、hat some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.ISO 4719 was prepared by Technical Committee ISO/TC 54, Essential oils.This third edition cancels and replaces the second edition (ISO 4719:1
12、999), which has been technically revised. ISO 2012 All rights reserved iiiBS ISO 4719:2012BS ISO 4719:2012Essential oil of spike lavender (Lavandula latifolia Medikus), Spanish type1 ScopeThis International Standard specifies certain characteristics of essential oil of spike lavender (Lavandula lati
13、folia Medikus), Spanish type, in order to facilitate assessment of its quality.2 Normative referencesThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the refe
14、renced document (including any amendments) applies.ISO/TR 210, Essential oils General rules for packaging, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density
15、 at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of miscibility in ethanolISO 1242, Essential oils Determination of acid valueISO 11024 (all parts), Essential oils General gui
16、dance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1essential oil of spike lavenderessential oil obtained by steam distillation of the flowering tops of Lavandula latifolia Medikus, of the Lamiaceae family, growing m
17、ainly in SpainNote 1 to entry: For information on the CAS number, see ISO/TR 21092.24 Requirements4.1 AppearanceClear mobile liquid.4.2 ColourLight yellow to orange yellow.INTERNATIONAL STANDARD ISO 4719:2012(E) ISO 2012 All rights reserved 1BS ISO 4719:2012ISO 4719:2012(E)4.3 OdourCharacteristic, e
18、arthy, more or less camphoraceous.4.4 Relative density at 20 C, d2020Minimum: 0,894Maximum: 0,9074.5 Refractive index at 20 CMinimum: 1,461Maximum: 1,4684.6 Optical rotation at 20 CBetween 7 and +24.7 Miscibility in ethanol 70 % volume fraction at 20 CIt shall not be necessary to use more than 3 vol
19、umes of ethanol 70 % volume fraction to obtain a clear solution with 1 volume of essential oil.NOTE Sometimes opalescence is observed on dilution.4.8 Acid valueMaximum: 2,04.9 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtai
20、ned the representative and characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil.Table 1 Chromatographic profileComponentMinimum Maximum% %Limonene
21、0,5 3,01,8-Cineole 16,0 39,0Camphor 8,0 16,0Linalool 34,0 50,0Linalyl acetate n.d.a1,6-Terpineol 0,2 2,0trans-Bisabolene 0,4 2,5NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A.aNot detectable.2 ISO 2012 All rights reservedBS ISO 4719:
22、2012ISO 4719:2012(E)4.10 FlashpointInformation on the flashpoint is given in Annex B.5 SamplingSampling shall be performed in accordance with ISO 212.Minimum volume of test sample: 25 ml.NOTE This volume allows each of the tests specified in this International Standard to be carried out at least onc
23、e.6 Test methodsRelative density at 20 C, d2020Determine the relative density in accordance with ISO 279.6.1 Refractive index at 20 CDetermine the refractive index in accordance with ISO 280.6.2 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592.6.3 Miscibility in etha
24、nol 70 % volume fraction at 20 CDetermine the miscibility in accordance with ISO 875.6.4 Acid valueDetermine the acid value in accordance with ISO 1242.6.5 Chromatographic profileDetermine the chromatographic profile in accordance with ISO 11024.7 Packaging, labelling, marking and storageThese items
25、 shall be in accordance with ISO/TR 210 and ISO/TR 211. ISO 2012 All rights reserved 3BS ISO 4719:2012ISO 4719:2012(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of spike lavender (Lavandula latifolia Medikus), Spanish type4 ISO 2012 All rig
26、hts reservedBS ISO 4719:2012ISO 4719:2012(E)KeyPeak identification Operating conditions1 -Pinene Column: capillary, fused silica; length 30 m; internal diameter 0,25 mm2 Camphene Stationary phase: poly(methyl siloxane)HP-1a3 Sabinene Film thickness: 0,25 m4 -Pinene Oven temperature: programmed tempe
27、rature from 75 C to 210 C at a rate of 3 C/min5 Myrcene6 p-Cymene Injector temperature: 250 C7 Limonene + 1,8-cineole Detector temperature: 250 C t time8 Linalool Detector: flame ionization type9 Camphor Carrier gas: helium10 Borneol Volume injected: 0,1 l11 Terpinen-4-ol Carrier gas flow rate: 1 ml
28、/min12 -Terpineol Split ratio: 1/25013 Linalyl acetate 14 Bornyl acetate 15 -Caryophyllene 16 trans-Bisabolene aHP-1 is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of th
29、is product.Figure A.1 Typical chromatogram taken on an apolar column ISO 2012 All rights reserved 5BS ISO 4719:2012ISO 4719:2012(E)KeyPeak identification Operating conditions1 -Pinene Column: capillary, fused silica; length 30 m; internal diameter 0,25 mm2 Camphene Stationary phase: poly(ethylene gl
30、ycol) Innowax 20 Ma3 -Pinene Film thickness: 0,15 m4 Sabinene Oven temperature: programmed temperature from 50 C to 210 C at a rate of 3 C/min, then from 210 C to 250 C at a rate of 40 C/min, then isothermal at 250 C for 5 min5 Myrcene6 Limonene Injector temperature: 250 C7 1,8-Cineole Detector temp
31、erature: 250 C t time8 p-Cymene Detector: flame ionization type9 Camphor Carrier gas: helium10 Linalool Volume injected: 0,1 l11 Linalyl acetate Carrier gas flow rate: 1 ml/min12 Bornyl acetate Split ratio: 1/25013 -Caryophyllene 14 Terpinen-4-ol 15 -Terpineol 16 Borneol 17 trans-BisaboleneaInnowax
32、20 M is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.2 Typical chromatogram taken on a polar column6 ISO 2012 All rights reservedBS ISO 4719:2012
33、ISO 4719:2012(E)Annex B (informative) FlashpointB.1 General informationFor safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products.A comparative study on the
34、 relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that:there is a wide variation in the chemical composition of essential oils;the volume of the sample needed in certain requirements would be too cost
35、ly for high-priced essential oils;as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for inform
36、ation, in order to meet the requirements of the interested parties.The equipment with which this value was obtained has to be specified.For further information see ISO/TR 11018.1B.2 Flashpoint of the essential oil of spike lavenderThe mean value is +60 C.NOTE Obtained with Setaflash1)equipment.1) Eq
37、uipment available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product. ISO 2012 All rights reserved 7BS ISO 4719:2012ISO 4719:2012(E)Bibliography1 ISO/TR 11018, Essential oils General guidance on the dete
38、rmination of flashpoint2 ISO/TR 21092, Essential oils Characterization8 ISO 2012 All rights reservedBS ISO 4719:2012BS ISO 4719:2012ISO 4719:2012(E) ISO 2012 All rights reservedICS 71.100.60Price based on 8 pagesThis page deliberately left blankBSI is the independent national body responsible for pr
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