1、BRITISH STANDARD BS ISO 4724:2004 Oil of cedarwood, Virginian type (Juniperus virginiana L.) ICS 71.100.60 BS ISO 4724:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 June 2004 BSI 30 June 2004 ISBN 0 580 43989 5 National foreword Thi
2、s British Standard reproduces verbatim ISO 4724:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential Oils, which has the responsibility to: A list of organizations represented on this committee can be obtain
3、ed on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Ele
4、ctronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to
5、 understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document co
6、mprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Co
7、mments Reference number ISO 4724:2004(E) OSI 4002INTERNATIONAL STANDARD ISO 4724 Second edition 2004-06-01 Oil of cedarwood, Virginian (Juniperus virginiana L.) Huile essentielle de bois de cdre, type Virginie (Juniperus virginiana L.)BSISO4724:2004IS:4274 O4002(E) DPlcsid Fremia ihTs PDF file may c
8、tnoian emdebt dedyfepcaes. In ccaocnadrw eith Aebods licensilop gnic,y this file mairp eb ynted iv roweb detu slahl ton ide ebtlnu deess the typefaces whice era hml era deddebicsnede to i dnanstlaled t noeh computfrep reormign tide ehtin.g In wodlnidaot gnhis file, trapise atpecc tiereht nser ehnops
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11、rehise specified, on trap fo this lbupictaion maeb y cudorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mecinahcal, inclidung tohpcoiypodna gn micrfoilm, wittuoh repmissii non writign from ietI rehSa Ot tsserdda eh ebolw or ISOs memreb i ydobn the cnuotrfo y ttseuqer ehe.r ISO cirypot
12、hg fofice saCe tsopale 65 eneG 1121-HC 02 av leT. 4 + 10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mail coirypthgiso.o gr We bwww.is.o gro Pulbisdehi n Switlrez dnaii ISO 4002 Allr ithgsr esedevrBSISO4724:2004IS:4274 O4002(E) I SO 4002 All irthgs ersedevr iiiForeword ISO (the International Organization fo
13、r Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the
14、 right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Internati
15、onal Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication
16、as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
17、ISO 4724 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 4724:1984), which has been technically revised. BSISO4724:2004INTENRATIONAL TSANDADR IS:4274 O4002(E)I SO 4002 All irthgs ersedevr 1Oil of cedarwood, Virginian (Jun
18、iperus virginiana L.) 1 Scope This International Standard specifies certain characteristics of the oil of cedarwood, Virginian (Juniperus virginiana L.), in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispen- sable for the applicatio
19、n of this document. For dated references, only the edition cited applies. For un- dated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General ru
20、les for la- belling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refrac- tive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential o
21、ils Evaluation of miscibility in ethanol ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in stan- dards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatogr
22、aphic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of cedarwood, Virginian essential oil obtained by steam distillation of the wood of Juniperus virginiana L., of the Cupressaceae family, growing i
23、n the United States of America from the South Eastern part of Virginia to Eastern Texas NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Slightly viscous liquid, sometimes containing crystals. 4.2 Colour Almost colourless to pale yellow. 4.3 Odour Characteristi
24、c, warm and woody. 4.4 Relative density at 20 C, 20 20 dMinimum: 0,941 0 Maximum: 0,965 0 4.5 Refractive index at 20 C Minimum: 1,501 0 Maximum: 1,510 0 4.6 Optical rotation at 20 C Between 36 and 16. BSISO4724:2004IS:4274 O4002(E) 2 I SO 4002 All irthgs ersedevr4.7 Miscibility in ethanol, 95% (volu
25、me fraction), at 20 C It shall not be necessary to use more than 5 volumes of ethanol, 95 % (volume fraction), to obtain a clear solution with 1 volume of essential oil. 4.8 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained
26、, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Component Minimu
27、m % Maximum % -Cedrene + -funebrene 20 35 -Cedrene + -caryophyllene 4 8 Thujopsene 10 25 Cuparene 1,5 7 Cedrol 16 25 Widdrol 2 5 NOTE 1 The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. NOTE 2 Cedrol and widdrol only appear as individual pe
28、aks in the chromatogram taken on a polar column. 4.9 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test
29、methods 6.1 Relative density at 20 C, 20 20 dSee ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 95% (volume fraction), at 20 C See ISO 875. 6.5 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, m
30、arking and storage See ISO/TR 210 and ISO/TR 211. BSISO4724:2004IS:4274 O4002(E) I SO 4002 All irthgs ersedevr 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of cedarwood, Virginian (Juniperus virginiana L.) Peak identification 1 -Cedrene + -f
31、unebrene 2 -Cedrene + -caryophyllene 3 Thujopsene 4 Cuparene 5 Cedrol 6 Widdrol Operating conditions Column: capillary; length 60 m; internal diameter 0,20 mm Stationary phase: poly(5 % diphenyl/95 % dimethyl siloxane) (SP-5 ) Film thickness: 0,20 m Oven temperature: temperature programming from 100
32、 C to 200 C at a rate of 3 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BSISO4724:2004IS:4274 O400
33、2(E) 4 I SO 4002 All irthgs ersedevrPeak identification 1 -Cedrene + -funebrene 2 -Cedrene + -caryophyllene 3 Thujopsene 4 Cuparene 5 Cedrol 6 Widdrol Operating conditions Column: capillary; length 60 m; internal diameter 0,20 mm Stationary phase: poly(ethylene glycol) (Carbowax ) Film thickness: 0,
34、20 m Oven temperature: temperature programming from 100 C to 200 C at a rate of 3 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 30 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogr
35、am taken on a polar column BSISO4724:2004IS:4274 O4002(E) I SO 4002 All irthgs ersedevr 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential
36、oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for stan- dardization purposes, given that: there is wide variation in the chemical composi- tion of essential
37、oils; the volume of the sample needed for certain requirements would be too costly for high- priced essential oils; as there are several different types of equip- ment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to gi
38、ve a mean value for the flashpoint in an informative annex to each International Standard in order to meet the require- ments of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oi
39、l of cedarwood, Virginian The mean value is +93 C. NOTE Obtained with “Setaflash“ equipment. BSISO4724:2004IS:4274 O4002(E) 6 I SO 4002 All irthgs ersedevrBibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092:2004, Essential oils Characte
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