1、October 2011 Translation by DIN-Sprachendienst.English price group 7No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS
2、 71.040.20!$ta“1819262www.din.deDDIN EN ISO 13132Laboratory glassware Petri dishes (ISO 13132:2011)English translation of DIN EN ISO 13132:2011-10Laborgerte aus Glas Petrischalen (ISO 13132:2011)Englische bersetzung von DIN EN ISO 13132:2011-10Verrerie de laboratoire Botes de Ptri (ISO 13132:2011)Tr
3、aduction anglaise de DIN EN ISO 13132:2011-10SupersedesDIN 12339:1984-04www.beuth.deDocument comprises pagesIn case of doubt, the German-language original shall be considered authoritative.1009.11 DIN EN ISO 13132:2011-10 2 A comma is used as the decimal marker. National foreword This standard has b
4、een prepared by Technical Committee ISO/TC 48 “Laboratory equipment” in collaboration with Technical Committee CEN/TC 332 “Laboratory equipment”, both secretariats are held by DIN, Germany. The responsible German body involved in its preparation was the Normenausschuss Laborgerte und Laboreinrichtun
5、gen (Laboratory Devices and Installations Standards Committee), Working Committee NA 055-01-03 AA Glasgerte und Apparate. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 719 DIN ISO 719 Amendments This standard differs from DIN 12339:19
6、84-04 as follows: a) two series, A and B, and further nominal sizes have been included; b) some dimensions have been modified; c) requirements relating to the construction have been extended and rendered more precise; d) flatness requirements and test have been supplemented; e) the designation has b
7、een changed. Previous editions DIN 12339: 1942-07, 1970-03, 1976-05, 1984-04 National Annex NA (informative) Bibliography DIN ISO 719, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classification EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 13132 July 2011 ICS 71
8、.040.20; 11.100.01 English Version Laboratory glassware - Petri dishes (ISO 13132:2011) Verrerie de laboratoire - Botes de Petri (ISO 13132:2011) Laborgerte aus Glas - Petrischalen (ISO 13132:2011) This European Standard was approved by CEN on 14 July 2011. CEN members are bound to comply with the C
9、EN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management C
10、entre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as
11、the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Roma
12、nia, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2011 CEN All rights of exploitation in any form and by any means reserved
13、worldwide for CEN national Members. Ref. No. EN ISO 13132:2011: EContents DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 2 Page Foreword3 1 Scope 4 2 Normative references 4 3 Types.4 4 Nominal sizes and series 4 4.1 Nominal sizes.4 4.2 Series 4 5 Designation 4 6 Material .5 7 Dimensions.5 7.1 Series
14、 A Petri dishes .5 7.2 Series B Petri dishes .6 8 Construction.6 8.1 Basic requirements6 8.2 Base and side.7 8.3 Edge 7 8.4 Flatness of bottom dishes 7 8.5 Performance requirements .7 9 Marking .7 Annex A (normative) Test method for flatness 8 Foreword This document (EN ISO 13132:2011) has been prep
15、ared by Technical Committee ISO/TC 48 Laboratory equipment” in collaboration with Technical Committee CEN/TC 332 “Laboratory equipment” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by e
16、ndorsement, at the latest by January 2012, and conflicting national standards shall be withdrawn at the latest by January 2012. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for i
17、dentifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
18、Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 13132:2011 has been approved by CEN as a EN ISO 13132:201
19、1 without any modification. “DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 3 1 Scope This International Standard specifies requirements and tests for glass Petri dishes intended for general laboratory purposes and microbiological work. 2 Normative references The following referenced documents are i
20、ndispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 719, Glass Hydrolytic resistance of glass grains at 98 C Method of test and classific
21、ation 3 Types Two types of Petri dishes are specified in this International Standard. Type 1 Thick-walled Petri dishes Type 2 Thin-walled Petri dishes 4 Nominal sizes and series 4.1 Nominal sizes Petri dishes shall have the following nominal sizes. 50, 60, 80, 100, 120, 150 and 200 millimetres NOTE
22、For Series B, nominal sizes refer to the external diameter of the bottom dish. 4.2 Series Petri dishes shall comply with the following dimensional requirements. Series A Dimensions in accordance with Table 1 Series B Dimensions in accordance with Table 2 5 Designation Petri dishes in accordance with
23、 this International Standard shall be designated by their nominal size followed by the type and the series. DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 4 Designation of a thick-walled Petri dish with nominal size of 120 mm, Type 1, Series A: Petri dish ISO 13132 120 20 1A If bottom dishes (2) and
24、 top dishes (1) are ordered separately, the following designations shall be used. Designation of a thick-walled bottom dish (2) of nominal size 120 mm, Type 1, Series A: Petri bottom dish ISO 13132 120 20 1A 2 Designation of a thick-walled top dish (1) of nominal size 120 mm, Type 1, Series A: Petri
25、 top dish ISO 13132 120 20 1A 1 6 Material Petri dishes shall be manufactured from transparent glass of hydrolytic resistance Class HBG 1, Class HBG 2 or Class HGB 3 in accordance with ISO 719. The glass shall be reasonably free from residual strain and from defects which might impair safety, durabi
26、lity or appearance such as mould marks and waviness. The glass shall not have any pronounced tint. 7 Dimensions 7.1 Series A Petri dishes Series A Petri dishes shall comply with the dimensions specified in Table 1. Key 1 top dish 2 bottom dish Key 1 top dish 2 bottom dish Figure 1 Thick-walled Petri
27、 dish Figure 2 Thin-walled Petri dish DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 5 Table 1 Dimensions for Series A Petri dishes Dimensions in millimetres Type 1 Thick-walled (see Figure 1) Type 2 Thin-walled (see Figure 2) Nominal size d1d201h1 1 h2 1 s d1 1 d2 1 h1 1,5 h2 1,5 s 0,3 50 12 47 45
28、9 14 1,3 60 20 1,5055+54 15 20 2,5 56 53 15 20 1,8 80 15 77 74 11 15 1,3 80 20 1,5071+70 15 20 2,5 76 73 15 20 1,8 100 15 15 15 100 20 1,5091+90 13 20 3 96 93 11,5 20 1,8 120 20 1,50111+110 15 20 3,5 116 113 15 20 1,8 150 30 20140+139 15 30 4 146 142 18 27 1,8 200 30 197 193 23 29 1,6 7.2 Series B P
29、etri dishes Series B Petri dishes shall comply with the dimensions specified in Table 2. For any pair of Series B Petri dishes, the difference between the internal diameter of the top dish and external diameter of the bottom dish shall be between 2 mm to 4 mm. Table 2 Dimensions for Series B Petri d
30、ishes Dimensions in millimetres Type 2 Thin-walled (see Figure 2) Nominal size d110+d201h101h210+s min. 50 17 53 50 15 17 1,0 80 17 83 80 15 17 1,0 100 17 103 100 15 17 1,5 150 20 153 150 17 20 1,5 200 20 203 200 20 20 1,5 8 Construction 8.1 Basic requirements Petri dishes shall be regular in shape
31、(see e.g. Figure 1) and smoothly finished. They shall be symmetrical about the axis which shall be perpendicular to the plane of the base. DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 6 8.2 Base and side The bases of both the bottom and top dishes shall be, as far as possible, in one plane surface
32、 and the sides shall be perpendicular to the base with a maximum deviation of 3. The bases may be given a slight crown to prevent rocking. 8.3 Edge The edges of the side wall of both bottom and top dishes shall be smoothly ground, fire-polished or clean pressed. The edges shall be in a plane paralle
33、l to their bases. If the edges are finished by burn-off process, there shall not be any tear-drop extending above the plane of cut-off in the bottom dish. In the top dish such protrusion up to 1 mm is permissible. 8.4 Flatness of bottom dishes The inside depth at any two points on the base of the bo
34、ttom dish shall not vary by more than 1,2 mm when measured in accordance with Annex A. 8.5 Performance requirements Petri dishes shall not show visible decrease of transparency, opalescence, iridescence, frosting or cracks after testing by the following procedures: minimum 15 min wet sterilization a
35、t minimum 120 C; minimum 1 h dry sterilization at minimum 160 C. 9 Marking Petri dishes shall be permanently and legibly marked on its sidewall with the following information: a) manufacturers and/or suppliers name and/or trade mark; b) optionally the number of this International Standard, “ISO 1313
36、2”. DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 7 Annex A (normative) Test method for flatness The following type test procedure applies for measurement of flatness of the inner surface of the Petri bottom dish. a) Affix a dial gauge to a stand. b) Place both the measuring equipment and the Petri
37、 dish under test on the same flat and firm surface. c) Lower the dial gauge in such a way that the pointer is pressed against the inner surface of the Petri dish so that some deflection is seen on the pointer. d) Then measure with the dial gauge the difference between the deepest and the highest point of the inner surface which should not exceed the value specified in 8.4. DIN EN ISO 13132:2011-10 EN ISO 13132:2011 (E) 8
