1、June 2010 Translation by DIN-Sprachendienst.English price group 14No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 1
2、3.040.20!$i-“1701056www.din.deDDIN ISO 16000-17Indoor air Part 17: Detection and enumeration of moulds Culture-based method (ISO 16000-17:2008)English translation of DIN ISO 16000-17:2010-06Innenraumluftverunreinigungen Teil 17: Nachweis und Zhlung von Schimmelpilzen Kultivierungsverfahren (ISO 1600
3、0-17:2008)Englische bersetzung von DIN ISO 16000-17:2010-06Air intrieur Partie 17: Dtection et dnombrement des moisissures Mthode par culture (ISO 16000-17:2008)Traduction anglaise de DIN ISO 16000-17:2010-06SupersedesDIN ISO 16000-17:2010-01www.beuth.deIn case of doubt, the German-language original
4、 shall be considered authoritative.This standard has been included in the VDI/DIN Handbook on air quality, Volume 5 .Document comprises 29 pages07.10 2 A comma is used as the decimal marker. Contents Page National foreword 3 National Annex NA (informative) Bibliography 5 Introduction.8 1 Scope 9 2 N
5、ormative references 9 3 Definitions 9 4 Principle. 11 5 Apparatus 11 6 Culture media and diluents 11 6.1 Dicloran 18 % glycerol agar (DG18 agar) .12 6.2 Malt-extract agar . 12 6.3 Potato dextrose agar 13 6.4 Saline solution 13 6.5 Saline solution with polysorbate 80 . 13 7 Procedure 14 7.1 General.
6、14 7.2 Processing of filters . 14 7.3 Incubation 15 7.4 Examination and counting. 15 7.5 Identification of mould species. 16 7.6 Calculation and expression of results 16 8 Test report . 19 Annex A (informative) Specific characteristics of mould spores . 20 Annex B (informative) Exchange of samples f
7、or validation of the cultivation method 23 Bibliography. 28 DIN ISO 16000-17:2010-06 National foreword International technical regulation pertaining to indoor air This standard has been prepared by Technical Committee ISO/TC 146 “Air quality”, Subcommittee SC 6 “Indoor air” (Secretariat: DIN, German
8、y), Working Group WG 10 “Fungi”. The responsible German body involved in its preparation was the Kommission Reinhaltung der Luft (KRdL) im VDI und DIN Normenausschuss (Commission on Air Pollution Prevention of VDI and DIN Standards Committee), Joint Working Committee Bioaerosole und biologische Agen
9、zien. The culture-based method specified in this standard is based on Guideline VDI 4253 Blatt 2 which has been prepared for the ambient air environment. The VDI procedure has been adapted to be suitable for indoor environments and has been validated on the basis of comprehensive analyses. Sampling
10、as part of the measurement method is given in DIN ISO 16000-16, Sampling by filtration, and in DIN ISO 16000-18, Sampling by impaction. General and substance-specific aspects which should be taken into account before and during sampling, as well as methods for determining individual substances or gr
11、oups of substances and microorganisms in indoor air are described in the various parts of DIN ISO 16000 and DIN EN ISO 16000. The DIN Standards corresponding to the International Standards referred to in clause 2 of this document are as follows: ISO 8199 DIN EN ISO 8199 ISO 16000-16 DIN ISO 16000-16
12、 ISO 16000-18 DIN ISO 16000-18 Technical Corrigendum 1:2009 to ISO 16000-17:2008-12-15 has been incorporated in this standard. In the national Bibliography, cross-references 1 to 3 have been added to DIN ISO 12219-1, DIN EN ISO 16017-1 and 2 and the titles/keys of Figures B.1 to B.3 have been modifi
13、ed. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. DIN shall not be held responsible for identifying any or all such patent rights. Amendments This standard differs from DIN ISO 16000-17:2010-01 as follows (applies to the German
14、version only): a) In Tables 4 and 5 the erroneously specified quantity of sodium chloride (NaCl) of 90,0 g has been corrected to read 8,5 g. Previous editions DIN ISO 16000-17: 2010-01 National technical regulation pertaining to indoor air General conditions and other aspects to be taken into consid
15、eration when planning indoor air measurements are described for individual substances and substance groups in the VDI 4300 series (see also “National Annex NA”). Many of these served as the sole basis for parts of the ISO 16000 series (e.g. ISO 16000-1, ISO 16000-2, ISO 16000-5, ISO 16000-7, ISO 160
16、00-12, ISO 16000-15, ISO 16000-16, ISO 16000-17 and ISO 16000-18). The specific procedures (sampling, analysis) are described in the VDI 4301 series for individual substances and substance groups. Methods which can be used for both indoor and ambient air measurements are described in further VDI gui
17、delines. 3DIN ISO 16000-17:2010-06 DIN ISO 16000 series and DIN EN ISO 16000 series consist of the following parts, under the general title Indoor air: DIN EN ISO 16000-1, General aspects of sampling strategy DIN EN ISO 16000-2, Sampling strategy for formaldehyde DIN ISO 16000-3, Determination of fo
18、rmaldehyde and other carbonyl compounds Active sampling method DIN ISO 16000-4, Determination of formaldehyde Diffusive sampling method DIN EN ISO 16000-5, Sampling strategy for volatile organic compounds (VOCs) DIN ISO 16000-6, Determination of volatile organic compounds in indoor and test chamber
19、air by active sampling on Tenax TAsorbent, thermal desorption and gas chromatography using MS/FID DIN EN ISO 16000-7, Sampling strategy for determination of airborne asbestos fibre concentrations DIN ISO 16000-8, Determination of local mean ages of air in buildings for characterizing ventilation con
20、ditions DIN EN ISO 16000-9, Determination of the emission of volatile organic compounds from building products and furnishing Emission test chamber method DIN EN ISO 16000-10, Determination of the emission of volatile organic compounds from building products and furnishing Emission test cell method
21、DIN EN ISO 16000-11, Determination of the emission of volatile organic compounds from building products and furnishing Sampling, storage of samples and preparation of test specimens DIN EN ISO 16000-12, Sampling strategy for polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs)
22、, polychlorinated dibenzofurans (PCDFs) and polycyclic aromatic hydrocarbons (PAHs) DIN ISO 16000-13, Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/PCDFs) Collection on sorbent-backed filters DI
23、N ISO 16000-14, Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/PCDFs) Extraction, clean-up and analysis by high-resolution gas chromatography and mass spectrometry DIN EN ISO 16000-15, Sampling s
24、trategy for nitrogen dioxide (NO2) DIN ISO 16000-17, Detection and enumeration of moulds Culture-based method DIN ISO 16000-18, Detection and enumeration of moulds Sampling by impaction DIN ISO 16000-23, Performance test for evaluating the reduction of formaldehyde concentrations by sorptive buildin
25、g materials DIN ISO 16000-24, Performance test for evaluating the reduction of volatile organic compounds and carbonyl compounds without formaldehyde concentrations by sorptive building materials 4DIN ISO 16000-17:2010-06 DIN ISO 16000-16, Detection and enumeration of moulds Sampling by filtration D
26、IN ISO 16000-25, Determination of the emission of semi-volatile organic compounds by building products Micro-chamber method The following parts are under preparation: DIN ISO 16000-19, Sampling strategy for moulds DIN ISO 16000-26, Sampling strategy for carbon dioxide CO2 DIN ISO 16000-27, Standard
27、method for the quantitative analysis of asbestos fibres in settled dust DIN ISO 16000-28, Sensory evaluation of emissions from building materials and products The following parts are planned: DIN ISO 16000-20, Detection and enumeration of moulds Sampling from house dust DIN ISO 16000-21, Detection a
28、nd enumeration of moulds Sampling from materials DIN ISO 16000-22, Detection and enumeration of moulds Molecular methods National Annex NA (informative) Bibliography DIN 50014, Climates and their technical application Standard atmospheres DIN EN ISO 8199, Water quality General guidance on the enumer
29、ation of micro-organisms by culture DIN EN ISO 9169, Air quality Definition and determination of performance characteristics of an automatic measuring system DIN ISO 12219-1, Indoor air Road vehicles Part 1: Whole vehicle test chamber Specification and method for the determination of volatile organi
30、c compounds in car interiors (under preparation) 1 DIN ISO 12884, Ambient air Determination of total (gas and particle phase) polycyclic aromatic hydrocarbons Collection on sorbent-backed filters with gas chromatographic/mass spectrometric analysis DIN V ENV 13005, Guide to the expression of uncerta
31、inty in measurement DIN EN 14412, Indoor air quality Diffusive samplers for the determination of concentrations of gases and vapours Guide for selection, use and maintenance DIN EN ISO 16017-1, Indoor, ambient and workplace air Sampling and analysis of volatile organic compounds by sorbent tube/ther
32、mal desorption/capillary gas chromatography Part 1: Pumped sampling 2 DIN EN ISO 16017-2, Indoor, ambient and workplace air Sampling and analysis of volatile organic compounds by sorbent tube/thermal desorption/capillary gas chromatography Part 2: Diffusive sampling 3 DIN ISO 16362, Ambient air Dete
33、rmination of particle-phase polycyclic aromatic hydrocarbons by high performance liquid chromatography 5DIN ISO 16000-17:2010-06 DIN ISO 16000-17:2010-06 6 DIN EN ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories VDI 2464 Blatt 1, Ambient air measurement
34、Indoor air measurement Measurement of polychlorinated biphynyls (PCBs) GC/MS method for PCB 28, 52, 101, 138, 153, 180 VDI 2464 Blatt 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated biphenyls (PCBs) HR-GC/HR-MS method for coplanar PCBs VDI 3484 Blatt 1, Gaseous ambie
35、nt air measurements Indoor-air pollution measurements Test gases measurements Measurement of the formaldehyde concentration with the sulfite pararosaniline method VDI 3484 Blatt 2, Gaseous ambient air measurements Indoor-air pollution measurements Measurement of the formaldehyde concentration with t
36、he acetylacetone method VDI 3498 Blatt 1, Ambient air measurement Indoor air measurement Measurement of polychlorinated dibenzo-p-dioxins and dibenzofurans Method using large filters VDI 3498 Blatt 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated dibenzo-p-dioxins and
37、 dibenzofurans Method using small filters VDI 4300 Blatt 1, Indoor-air pollution measurement General aspects of measurement strategy VDI 4300 Blatt 2, Indoor-air pollution measurement Measurement strategy for polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins (PCDDs), polychl
38、orinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) VDI 4300 Blatt 4, Indoor air pollution measurement Measurement strategy for pentachlorophenol (PCP) and -hexachlorocyclohexane (lindane) in indoor air VDI 4300 Blatt 5, Indoor air pollution measurement Measurement strategy for nitro
39、gen dioxide (NO2) VDI 4300 Blatt 6, Measurement of indoor air pollution Measurement strategy for volatile organic compounds (VOCs) VDI 4300 Blatt 7, Indoor-air pollution measurement Measurement of indoor air change rate VDI 4300 Blatt 8, Measurement of indoor air pollution Sampling of house dust VDI
40、 4300 Blatt 9, Measurement of indoor air pollution Measurement strategy for carbon dioxide (CO2) VDI 4300 Blatt 10, Measurement of indoor air pollution Measurement strategies for determination of mould fungi in indoor environment VDI 4301 Blatt 1, Indoor air pollution measurement Determination of th
41、e concentration of nitrogen dioxide Manual photometric method (Saltzman) VDI 4301 Blatt 2, Indoor air pollution measurement Measurement of pentaclorophenol (PCP) and -hexachlorocyclohexane (lindane) GC/MS-method VDI 4301 Blatt 3, Measurement of indoor air pollution Measurement of pentaclorophenol (P
42、CP) and -hexachlorocyclohexane (lindane) GC/ECD method VDI 4301 Blatt 4, Measurement of indoor air pollution Measurement of pyrethroids and piperonyl butoxide in air, house dust and solvent wipe samples VDI 4301 Blatt 5, Measurement of indoor air pollution Measurement of flame retardants and plastic
43、izers based on phosphor organic compounds Phosphoric acid ester The following VDI Guidelines are under preparation: VDI 2464 Blatt 3, Ambient air measurement Indoor air measurement Measurement of polybrominated diphenyl ethers (flame retardants) VDI 4300 Blatt 11, Measurement of indoor air pollution
44、 Measurement strategies for fine dust VDI 4301 Blatt 6, Measurement of indoor air pollution Measurement of phthalates 7DIN ISO 16000-17:2010-06 Introduction Mould is a common name for filamentous fungi from different taxonomic groups Zygomycetes, Ascomycetes (Ascomycota), Deuteromycetes. They form a
45、 mycelium (hyphae) and spores namely conidiospores (conidia), sporangiospores or ascospores by which they become visible macroscopically. Most spores are in the size range 2 m to10 m, some up to 30 m and a very few up to 100 m. Spores of some mould genera are small and become airborne very easily (e
46、.g. Aspergillus, Penicillium) while others are bigger and/or embedded in a slime matrix (Stachybotrys, Fusarium) and less mobile. Mould spores are widely distributed in the outdoor environment and, therefore, also occur in varying concentrations indoors. Growth of moulds in indoor environments, howe
47、ver, should be considered a public health problem because epidemiological studies have revealed that dampness and/or mould growth in homes and health impairment of occupants are closely related. Standardised methods for sampling, detection and enumeration of moulds including standards for sampling s
48、trategies are important for comparative assessment of mould problems indoors. Before taking any measurements, a measurement strategy is required. The procedure specified in this part of ISO 16000 is based on VDI 4253-2 5and VDI 4300-106. Indoor air Part 17: Detection and enumeration of moulds Cultur
49、e-based method 8DIN ISO 16000-17:2010-06 WARNING The use of this part of ISO 16000 may involve hazardous materials, operations and equipment. This part of ISO 16000 does not purport to address any safety problems associated with its use. It is the responsibility of the user of this part of ISO 16000 to establish appropriate safety and health practices and
