1、BRITISH STANDARD Soil improvers and growing media Determination of nitrogen Part 2: Dumas method The European Standard EN 13654-2:200 1 has the status of a British Standard ICs 65.080 BS EN 1 3654-2 1200 1 Incorporating Corrigendum No. I present to the responsible European committee any enquiries on
2、 the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. - A list of organizations represented on this committee can be obtained on request to its secretary. Cross-references The British S
3、tandards which implement international or European publications referred to in this document may be found in the BSI Standards Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Find” facility of the BSI Standards Electronic Catalogue. A British Sta
4、ndard does not purport to include all the necessary provisions of a contract. Users ofBritish Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. 5 December 2001 Corrects the header on the EN contents
5、and EN foreword pages. This British Standard. havinp. - been prepared under the direction of the Consumer Products and Services Sector Summary of pages This document comprises a front cover, an inside front cover, the EN title page, Policy andstrategy Committee, was published under the authority of
6、the Standards Policy and Strategy Committee on 09 November 2001 pages 2 to 8, an inside back cover and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. O BSI 5 December 2001 ISBN O 580 38265 6 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NO
7、RM EN 13654-2 September 2001 ICs 65.080 English version Soil improvers and growing media - Determination of nitrogen - Part 2: Dumas method Amendements du sol et supports de culture - Dtermination de lazote - Partie 2: Mthode de Dumas Bodenverbesserungsmittel und Kultursubstrate - Bestimmung von Sti
8、ckstoff - Teil 2: Verfahren nach Dumas This European Standard was approved by CEN on 11 August 2001. CEN members are bound to comply with the CENKENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-
9、to-date lists and bibliographical references concerning such national standards may be obtained on application to the Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under
10、the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembou
11、rg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION EUROPISCHES KOMITEE FR NORMUNG COMIT EUROPEN DE NORMALISATION Management Centre: rue de Stassart, 36 B-1050 Brussels O 2001 CEN All rights of exploitation in any form and by any m
12、eans reserved worldwide for CEN national Members. Ref. No. EN 13654-2:2001 E EN 13654-212001 (E) Contents Page Foreword . . . . . . . . . 3 . 4 1 Scope 2 Normative references . 4 3 Terms and definitions . 4 . 4 4 . 4 5 6 . 5 Pri nci p I e . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
13、 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Apparatus . . . . . . . . . 7 Test sample 5 Procedure . . 5 8 . 5 9 10 Expression of results . 6 11 6 12 Test Report . 6 Annex A (informative) Results of an interlaboratory trial to determine nitrogen (Dumas metho
14、d)7 Precision . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bibliograph y . 8 2 EN 13654-212001 (E) Foreword This European Standard has been prepared by Technical Committee CEN/TC 223 “Soil improvers and growing media“, the secretariat of which is held by BSI.
15、 This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by March 2002, and conflicting national standards shall be withdrawn at the latest by March 2002. Annex A is informative. According to the CENKENELEC
16、Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzer
17、land and the United Kingdom. 3 EN 13654-2:2001 (E) SAFETY PRECAUTIONS - Care should be taken when handling samples that may contain sharps or is of a dusty nature. 1 Scope This European Standard specifies a method for the determination of nitrogen in soil improvers and growing media. The dry combust
18、ion method was developed originally as a manual method by Dumas 5. Its application is improved greatly due to the use of modern automated equipment and is applicable to all forms of nitrogen. The method is not applicable to liming materials and preformed materials such as mineral wool slabs and foam
19、 slabs. NOTE the products concerned. The requirements of the standard may differ from the national legal requirements for the declaration of 2 Normative references This European Standard incorporates by dated or undated reference, provisions from other publications. These normative references are ci
20、ted at the appropriate places in the text, and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references, the latest edi
21、tion of the publication referred to applies (including amendments). EN 13040:1999, Soil improvers and growing media - Sample preparation for chemical and physical test, determination of dry matter content, moisture content and laboratory compacted bulk density. EN 13654-1, Soil improvers and growing
22、 media - Determination of nitrogen - Part I: Modified Kjeldahl method. 3 Terms and definitions For the purposes of this standard the terms and definitions given in EN 13040 apply. 4 Principle The nitrogen content of the sample, pre-treated according to EN 13040, is determined by heating to a tempera
23、ture of at least 900 OC in the presence of oxygen gas. Mineral and organic nitrogen compounds are oxidized and/or volatilized and determined as described by the manufacturer. 5 Reagents 5.1 General Use only reagents of recognized analytical grade. 5.2 For reduction, oxidation, removal andlor fixing
24、of combustion gases that interfere with the analysis, refer to the manufacturer?s instructions. 5.3 Calibration substances, pure substances of known nitrogen content, for example acetanilide (C8H9NO), L-aspartic acid (C4H7N04), or amino acids of known composition. NOTE content as possible. The total
25、 content of nitrogen of the calibration substance should be as similar to the sample nitrogen 4 EN 13654-212001 (E) 6 Apparatus Usual laboratory apparatus, and in particular the following 6.1 Analytical balance, capable of weighing accurately to 1 ,O mg. 6.2 Dumas apparatus, used to determine the to
26、tal nitrogen content by combustion of the sample at a temperature of at least 900 OC, including a detector for measuring the nitrogen gas formed. Consult the manufacturers manual for the setting up and use of the apparatus. 6.3 Containers, of various size as applicable to the instrument. 7 Test samp
27、le Prepare the test sample in accordance with EN 13040:1999, clause 9 Losses of nitrogen can occur with samples of high ammonium-N content in alkaline situations and particularly when these samples are dried. If the sample as received contains (or is suspected to contain) 500 mg/l NH4 -N, as determi
28、ned by EN 13651 2 or EN 13652 3, analyze the fresh material using the method described in EN 13654-1. 8 Procedure 8.1 Calibration of the apparatus Calibrate the apparatus as described in the manufacturers manual. For the purpose of calibration or establishing a calibration scale, use one of the subs
29、tances given in (5.3). 8.2 Determination of the nitrogen content The amount of test sample for analysis depends on the expected content of nitrogen and on the sample capacity of the apparatus being used. Weigh out m1 g of the dried ground sample or sub- sample into a container (6.3). Carry out the a
30、nalyses in accordance with the manufacturers manual. Normally the primary results are given as mg N (XI) per mass of air dry ground test sample used (ml). 8.3 Residual moisture The moisture in the analyzed sample (8.2) is determined in accordance with EN 13040:1999, clause 1 O, using 1 O g of the dr
31、ied sample obtained from EN 13040:1999, clause 9. NOTE Pores within the test sample are filled with air, and therefore, with nitrogen gas. Nitrogen gas can also enter the combustion cell when it is opened for sample exchange. Both facts can lead to an overestimation of the total N content of soil im
32、provers or growing medium if there has been insufficient purging by gas before the combustion step. 8.4 Laboratory compacted bulk density Determine the laboratory compacted bulk density in accordance with EN 13040:1999, annex A. 9 Calculation The nitrogen content (wn) in milligrams per gram dry matt
33、er basis, is calculated using the formula: 5 EN 13654-2:2001 (E) where: w, is the content of nitrogen in milligrams per gram of dried ground test sample; XI is the primary result in mg nitrogen; rn1 is the mass, in grams, of dried ground test sample used for analysis. 10 Expression of results Report
34、 all results on a mass/mass basis calculated to a dry matter basis. 11 Precision The repeatability and reproducibility of the nitrogen content in separately prepared samples should be in accordance with Table A.1. A summary of the results of an interlaboratory trial to determine the precision of the
35、 method, in accordance with IS0 5725 I, is given in annex A. NOTE matrices other than those tested. The values derived from this interlaboratory trial may not be applicable to concentrations and 12 Test Report The test report can be prepared separately or in conjunction with the test report of the s
36、ubsequent analytical method. The test report shall include the following information: a) a reference to the European Standard; b) a complete identification of the sample; c) all the analytical methods used; d) a statement whether ground or un-ground sample has been used; e) the results of the determ
37、ination expressed as mass/mass on dry matter basis; f) the laboratory compacted bulk density; g) moisture content; h) any details not specified in the European Standard, or which are optional, as well as any other factor, which may have affected the results. 6 n W U z W L * O F 5 m U C m C m al Q 3
38、v) 2 5 W .- C U al r o al Q v) v) 3 U al o .- .- 2 g al 5 + v) al O O m N m U C m + + C- .- + .- F 5 ,o E o L al al + .- 5 C m al Q 3 W al O v) al o Q v) 3 m al 2 5 cc .- 5 & U C 3 b o o C U al N C m r .- .- v) m m 3 - .- L + 8 L & + O a m - + C .- vj al Q al + - E m v) X v) C U O .- .- 5 E E v) 3 a
39、l o, C v) 3 C al C n 5 .- + + 8 2 5 C al o, + .- C al U al C .- + Fu al U r a al a - P C C al o, v) al .- .- .- 8 L + O a m O a 3 C al - cc & E 5 - m v) .- L + .- 5 C - h o O 5 E E u) 3 o L v C a rn * .- C O C O Q C ic .- * .- E a a o a * 5 L ic O Q - .- L * P E O O n Q a C a * - L * .- 5 ic O u) 3
40、u) a * - E 5 P E 4 ic O E 3 u) I 4 2 a n - Co m 0- O W rn- - rn O - o 0- r EN 13654-212001 (E) Bibliography I IS0 5725: 1994, Accuracy (trueness and precision) of measurement methods and results. 2 EN 13651, Soil improvers and growing media - Extraction of calcium chloride/DTPA (CAT) soluble nutrien
41、ts and elements. 3 EN 13652, Soil improvers and growing media - Extraction of water soluble nutrients and elements. 4 Dumas, J.B.A. (1831), Procds de lanalyse organique. Ann. Chim. Phys. 247, 198-213. 8 BS EN 1 3654-2 1200 1 B SI 389 Chiswick High Road London W4 4AL BSI is the independent national b
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