1、BRITISH STANDARD BS EN ISO 8655-2:2002 Piston-operated volumetric apparatus Part 2: Piston pipettes ICS 17.060 Incorporating Corrigendum December 2008National foreword This British Standard is the UK implementation of EN ISO 8655-2:2002. It is identical with ISO 8655-2:2002, incorporating corrigendu
2、m December 2008. This part of BS EN ISO 8655:2002, together with Parts 3, 4 and 5, supersedes BS 7653-2:1993 which is withdrawn. This part of BS EN ISO 8655:2002, together with Parts 1 and 6, supersedes BS 6018:1991 and BS 7532:1991 which are withdrawn. The start and finish of text introduced or alt
3、ered by corrigendum is indicated in the text by tags. Text altered by ISO corrigendum December 2008 is indicated in the text by . The UK participation in its preparation was entrusted by Technical Committee LBI/36, Laboratory glassware and related apparatus, to Subcommittee LBI/36/1, General and vol
4、umetric, glass and plastics apparatus. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a Brit
5、ish Standard cannot confer immunity from legal obligations. BS EN ISO 8655-2:2002 Amendments/corrigenda issued since publication Date Comments 30 June 2009 Implementation of ISO corrigendum December 2008 This British Standard, having been prepared under the direction of the Materials and Chemicals S
6、ector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 22 October 2002 BSI 2009 ISBN 978 0 580 66189 1EUROPEANSTANDARD NORMEEUROPENNE EUROPISCHENORM ENISO86552 September2002 ICS17.060 Englishversion PistonoperatedvolumetricapparatusPa
7、rt2:Pistonpipettes (ISO86552:2002) AppareilsvolumtriquespistonPartie2:Pipettes piston(ISO86552:2002) VolumenmessgertemitHubkolbenTeil2: Kolbenhubpipetten(ISO86552:2002) ThisEuropeanStandardwasapprovedbyCENon13July2002. CENmembersareboundtocomplywiththeCEN/CENELECInternalRegulationswhichstipulatethec
8、onditionsforgivingthisEurope an Standardthestatusofanationalstandardwithoutanyalteration.Uptodatelistsandbibliographicalreferencesconcernings uchnational standardsmaybeobtainedonapplicationtotheManagementCentreortoanyCENmember. ThisEuropeanStandardexistsinthreeofficialversions(English,French,German)
9、Aversioninanyotherlanguagemadebytra nslation undertheresponsibilityofaCENmemberintoitsownlanguageandnotifiedtotheManagementCentrehasthesamestatusasthe official versions. CENmembersarethenationalstandardsbodiesofAustria,Belgium,CzechRepublic,Denmark,Finland,France,Germany,Greece, Iceland,Ireland,Ita
10、ly,Luxembourg,Malta,Netherlands,Norway,Portugal,Spain,Sweden,SwitzerlandandUnitedKingdom. EUROPEANCOMMITTEEFORSTANDARDIZATION COMITEUROPENDENORMALISATION EUROPISCHESKOMITEEFRNORMUNG ManagementCentre:ruedeStassart,36B1050Brussels 2002CEN Allrightsofexploitationinanyformandbyanymeansreserved worldwide
11、forCENnationalMembers. Ref.No.ENISO86552:2002EForeword Thisdocument(ISO86552:2002)hasbeenpreparedbyTechnicalCommitteeISO/TC48 “Laboratoryglasswareandrelatedapparatus“incollaborationwithTechnicalCommittee CEN/TC332“Laboratoryequipment“,thesecretariatofwhichisheldbyDIN. ThisEuropeanStandardshallbegive
12、nthestatusofanationalstandard,eitherbypublication ofanidenticaltextorbyendorsement,atthelatestbyMarch2003,andconflictingnational standardsshallbewithdrawnatthelatestbyMarch2003. AccordingtotheCEN/CENELECInternalRegulations,thenationalstandardsorganizationsof thefollowingcountriesareboundtoimplementt
13、hisEuropeanStandard:Austria,Belgium, CzechRepublic,Denmark,Finland,France,Germany,Greece,Iceland,Ireland,Italy, Luxembourg,Malta,Netherlands,Norway,Portugal,Spain,Sweden,Switzerlandandthe UnitedKingdom. Endorsementnotice ThetextoftheInternationalStandardISO86552:2002hasbeenapprovedbyCENasa EuropeanS
14、tandardwithoutanymodifications. NOTENormativereferencestoInternationalStandardsarelistedinAnnexZA(normative). ENISO86552:2002INTERNATIONAL STANDARD ISO 86552 Firstedition 20020915 Referencenumber ISO 86552:2002(E) Pistonoperatedvolumetricapparatus Part 2: Pistonpipettes Appareilsvolumtriquespiston P
15、artie 2:Pipettespiston ENISO86552:2002ISO 55682:2)E(200 ii ISO 2002 Arllithgrseresvde FDPdicslaimre ThisFDPfamelitnocytdeddebmeniayfep.secaInroccatiwecnadAhycilopgnisnecilsebod,thfsimeliarpebytnieivrodewbde tusllahtoneb tidesselnudettehyfepsecahwichraemeileradeddebctdesneotsnidnalatnodelmocehtupreep
16、frromigntideehti.gnInodaolnw tgnidhisfile, rapticcasetpetrehietnehserilibisnoptyfotonfnirisebodAgnignilopgnisnecilcy.TIehSOtneCraceSlrteraiatcatpeconsillibaityinthisaera. ebodAsitarmedarakfodASebotsysmeInroctarop.de teDailfostfosehtwareprtcudostdesuocrtaetehFDPsiffebnacelitnidnuoGehlareneInfotaleriv
17、etotfeh;elitehPDFrc taerapnoimateers weertpofdezimiorrptnii.gnvErecyaeebsahertnaktneorusnetetahtfehielistiusbafelobesuryISOmerebmseidob.Inthe kilnuleyevtne ttahaborpmeltalerigntotifsi,dnuosaelpfnieormtehtneCSlaretercrataitathserddaesigvneolebw. ISO 2002 ArlliesersthgrvsselnU.detorehcepsesiwfi,deirap
18、ontihtfosbupliactimnoarperebyrodecudotuzilinanideyformbroyna snaemy, tcelernahcemrocinoicla,inclidungtohppocoyingmdnaicrfomli,tiwtuohpermininoisswrtifgnirmoietIrehSOtatserddaehebs loworSIsO memrebydobintocehtnuryfotrehtseuqeer. ISOcopyrithgfofiec tsopesaCael65G1121HCeneva02 Te.l +22141094711 Fax +14
19、472290974 Eliam copyrightsio.ch Web wwwsi.oc.h PrnidetniSztiwerldna ii ENISO86552:2002OSI 55682002:2)E( ISO 2002 rllAithgsreservde iii Contents Page 1 Scope. 1 2 Normativereferences . 1 3 Termsanddefinitions 2 4 Principleofoperation 2 5 Design 2 6 Type,designation 5 7 Metrologicalperformancerequirem
20、ents . 5 8 Marking. 8 Annexes A Informationtobeprovidedbythesupplierfortheuserofpistonpipettesandtips . 9 B Possiblesourcesoferrorforpistonpipetteswithairinterface 10 Bibliography. 11 iii ENISO86552:2002Foreword ISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstand
21、ardsbodies(ISO memberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnical committees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhas therighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnongovernmental,
22、in liaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnical Commission(IEC)onallmattersofelectrotechnicalstandardization. InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part 3. DraftInternationalStandardsadoptedbythetechnica
23、lcommitteesarecirculatedtothememberbodiesforvoting. PublicationasanInternationalStandardrequiresapprovalbyatleast75 %ofthememberbodiescastingavote. AttentionisdrawntothepossibilitythatsomeoftheelementsofthispartofISO 8655maybethesubjectofpatent rights.ISOshallnotbeheldresponsibleforidentifyinganyora
24、llsuchpatentrights. InternationalStandardISO 86552waspreparedbyTechnicalCommitteeISO/TC 48, Laboratoryglasswareand relatedapparatus,SubcommitteeSC 1,Volumetricinstruments. ISO 8655consistsofthefollowingparts,underthegeneraltitlePistonoperatedvolumetricapparatus : Part 1: Terminology,generalrequireme
25、ntsanduserrecommendations Part 2: Pistonpipettes Part 3: Pistonburettes Part 4: Dilutors Part 5: Dispensers Part 6: Gravimetricmethodsforthedeterminationofmeasurementerror Annex AformsanormativepartofthispartofISO 8655.Annex Bisforinformationonly. iv ENISO86552:2002 Part 7: Non-gravimetric methods f
26、or the assessment of equipment performance OSI 55682002:2)E( ISO 2002 rllAithgsreservde v Introduction ISO 8655addressestheneedsof: suppliers,asabasisforqualitycontrolincluding,whereappropriate,theissuanceofsuppliersdeclarations; testhousesandotherbodies,asabasisforindependentcertification; usersoft
27、heequipment,toenableroutinecheckingofaccuracy. Thetestsspecifiedshouldbecarriedoutbytrainedpersonnel. v ENISO86552:2002 blankANRETNIITOTSLANDNADRA OSI 55682002:2)E( ISO 2002 rllAithgsreservde 1 Pistonoperatedvolumetricapparatus Part 2: Pistonpipettes 1 Scope ThispartofISO 8655specifies metrologicalr
28、equirements, maximumpermissibleerrors, requirementsformarkingand informationtobeprovidedforusers, forairdisplacement(type A)andpositivedisplacement(type D)singlechannelandmultichannelpistonpipettes, completewiththeirselectedtip(s)andanyotheressential,consumableparts,designedtodelivertheirspecified n
29、ominalvolume(Ex). NOTE GeneralrequirementsanddefinitionsoftermsofpistonoperatedvolumetricapparatusaregiveninISO86551. bythesupplier,analyticalandmeasuringequipmentqualityassurancebytheuser)seeISO 86556oralternativetestmethods. Forsafetyrequirementsofelectricallypoweredpistonpipettes,seeIEC 610101. 2
30、 Normativereferences Thefollowingnormativedocumentscontainprovisionswhich,throughreferenceinthistext,constituteprovisionsof thispartofISO 8655.Fordatedreferences,subsequentamendmentsto,orrevisionsof,anyofthesepublicationsdo notapply.However,partiestoagreementsbasedonthispartofISO 8655areencouragedto
31、investigatethe possibilityofapplyingthemostrecenteditionsofthenormativedocumentsindicatedbelow.Forundatedreferences, thelatesteditionofthenormativedocumentreferredtoapplies.MembersofISOandIECmaintainregistersof currentlyvalidInternationalStandards. ISO 3696,Waterforanalyticallaboratoryuse Specificat
32、ionandtestmethods ISO 86551:2002, Pistonoperatedvolumetricapparatus Part 1:Terminology,generalrequirementsanduser recommendations ISO 86556:2002,Pistonoperatedvolumetricapparatus Part 6:Gravimetricmethodsforthedeterminationof measurementerror 1 ENISO86552:2002 methodssuchasphotometricandtitrimetricm
33、ethodsaregiveninISO 86557. Forallothertests(e.g.qualityassu rance Conformitytesting(typeevaluation)ofpistonoperatedvolumetricapparatusisgiveninISO 86556. Alternativetest 3 Termsanddefinitions ForthepurposesofthispartofISO 8655,thetermsanddefinitionsgiveninISO 86551andthefollowingapply. 3.1 nominalvo
34、lume variablevolumepistonpipettegreatestvolumeselectablebytheuserandspecifiedbythemanufacturer NOTE Thismeansthatavariablevolumepistonpipettewithausefulvolumerange(seeISO 86551)of to hasthe nominalvolumeof . 3.2 nominalvolume multichannelpistonpipette greatestvolumeselectablebytheuserandspecifiedbyt
35、hemanufacturerperchannel 4 Principleofoperation Thetipmadeofplasticorglassisattachedtothepistonpipette.Withthepistonattheloweraspirationlimit,thetipis dippedintotheliquidtobedispensedasameasuredvolume.Whenmovedtotheupperaspirationlimit,thepiston aspiratestheliquid.Theliquidvolumetobedispensedisthene
36、xpelledbydepressingorslidingthepistonbetween thevolumedefininglimits.Someairdisplacementpistonpipettes(see 5.1,type A)haveanextraairvolumewhich canbeusedtoexpelthelastdropofliquid. SeealsoFigure 1. 5D e s i g n 5.1 Typesofpistonpipette Apistonpipettemaybedesignedasfollows: fixedvolume,designedbythem
37、anufacturertodispenseonlyitsnominalvolume,forexample ; variablevolume,designedbythemanufacturertodispensevolumesselectablebytheuserwithinitsspecified usefulvolumerange,forexamplebetween and . Thepistonmay eitherhaveabodyofaircontainedbetweenthepistonandthesurfaceoftheliquid(airdisplacement type A);
38、orbeindirectcontactwiththesurfaceoftheliquid(positiveordirectdisplacement type D). Inthecaseofthetype Dpistonpipette,eithertheplungerorthecapillary,orbothmaybereusable(type D1)or disposable(type D2). SeeFigure 1fordetails. 5.2 Adjustment 5.2.1 Apistonpipetteshallbeadjustedbyitsmanufacturerforthedeli
39、very(Ex)ofitsnominalvolume(orselected volume,inthecaseofavariablevolumemodel),forthestandardreferencetemperatureof ,arelativeair humidityof andabarometricpressureof ( ),usinggrade 3waterasspecifiedinISO 3696. 10 l 100 l 100 l 100 l 10 l 100 l 20 C 50 % 101 kPa 1,01 bar 2 ENISO86552:2002 When a pisto
40、n pipette is required for use in a country which has adopted a standard reference temperatureof 27 C (the alternative recommended for tropical use), this figure shall be substituted for 20 C. OSI 55682002:2)E( ISO 2002 rllAithgsreservde 3 5.2.2 Somepistonpipetteshaveprovisionforadjustmentbytheuserwh
41、en,forexample,itisfoundinroutine calibrationthatthevolumedeliveredisnotwithinspecification.Suchuseradjustmentshallbemadeaccordingtothe manufacturersinstructionsandbyreferencetothegravimetrictestmethodspecifiedinISO 86556. Anypistonpipettesoadjustedshallhaveclear,visibleevidencethattheinitialadjustme
42、nthasbeenmodified.This informationshallalsoberecordedonanycertificateofconformityandtheprocedureshouldbeincludedintheusers qualitymanual. 5.2.3 Somepistonpipettesaredesignedtohavetheirfactorypresetadjustmentalteredbytheusersothattheywill dispensetheirspecifiedvolumewhenusedwithliquidswithphysicalpro
43、pertiesdifferingfromthoseofwater(see annex Bfordetails).Insuchcases,thedesignshallpreventunintentionalreadjustment.Suchuseradjustmentshall bemadeaccordingtothemanufacturersinstructionsandbyreferencetothegravimetrictestmethodspecifiedin ISO 86556,butusingtheselectedliquidinsteadofwater. Iftheuserreadjuststhepistonpipette,itshallbeclearlyandunequivocallyindicatedontheoutsideofthepiston pipettethatreadjustmenthasbeeneffected.Theusershallmarktheoutsideofthepistonpipettewit
