GOST EN 14352-2013 Foodstuffs Determination of fumonisins B1 and B2 in maize based foods HPLC method with immunoaffinity column clean up《食品 玉米基食物中伏马菌素B1和B2的测定 免疫亲和柱层净化的高效液相色谱 (HPLC.pdf

1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) EN 14352 2013 1 2 (EN 14352:2004, IDT) 2014 EN 14352 2013 II , - 1.0 92 . 1.22009 . -, . , , , . 1 - ( ) - - ( ) , 4 2 ( 335) 3 , ( 27 2013 . 57-) : ( 3166) 00497 ( 3166) 00497 AM BY KG MD RU UZ - 4 N 14352:2004 Foodstuf

2、fs Determination of fumonisin B1and B2in maize based foods. HPLC method with immunoaffinity column clean up ( . 1 2 . ). (en) , - , . (IDT) 5 08.11.2013 1515- EN 14352-2013 - 1 2015 . 6 EN 14352 2013 III - , . () - - . , , 2014 -, - EN 14352 2013 IV 1 . 1 2 1 3 . 1 4 . 1 5 . 3 6 . 4 7 4 8 . 6 9 . 7

3、10 8 () . 9 () 12 . 14 EN 143522013 1 1 2 Foodstuffs. Determination of fumonisins B1and B2 in maize based foods. HPLC method with immunoaffinity column clean up 201507 01 1 1 2 - () 1, 2, 3. , 4 - . 1 323 1414 / 2 - 90 558 /. 2 - . . -, . EN ISO 3696 Water for analytical laboratory use Specification

4、 and test methods (ISO 3696:1987) ( . ) 3 , . - . -, , - 2- - . - () - . 4 4.1 - - EN 14352 2013 2 N ISO 3696. - . 4.2 . 4.3 . 4.4 (34) = 85 %. 4.5 . 4.6 2-. 4.7 , (NaH2PO4 2H2O) = 0,1 /3 15,6 (NaH2PO4 2H2O) 1 3. 4.8 , (Na24O7 10H2O) = 0,1 /3 3,8 (Na24O7 10H2O) 100 3. 4.9 . 4.10 (Na2HPO4). 4.11 (KH2

5、PO4). 4.12 . 4.13 . 4.14 25 4.3, 25 4.2 50 . 4.15 - (H3CN) = 50 % 50 4.3 50 . 4.16 - 8,0 4.9, 1,2 4.10, 0,2 4.11 0,2 4.12 990 3. 7,0 . pH 4.13, 1 3. - . 4.17 , - , 1 2, - 5 - 90 % 5 1 2 - - , - 10 %. . 4.18 77 4.2 23 - 4.7. 3,35 - 4.4. 5.14. - . 4.19 40 4.5 1 3 4.2. 5 3 4.8 50 32- 4.6, . EN 14352 20

6、13 3 1 - . 4.20 1 2, 1 2 - 4.15 50 /3. 4 , 6 . 4.21 1 2, 5 3 1000 3 1 500 3 2. - 4.15, . 1 10 /3, 2 5 /3. 4 , 6 . . 4.22 1 2, 1 2 5 3 1. - 4.15, . - 4 , 6 . - . 1 1 2 , -, 3 - -, -, 3 - 1 -, /3 - 2 -, /31 50 4950 0,10 0,050 2 125 4875 0,25 0,125 3 500 4500 1,00 0,500 4 1000 4000 2,00 1,000 5 5.1 -,

7、, . 5.2 . 5.3 250 3 . 5.4 , 2500g. 5.5 20 25 . 5.6 11 . 5.7 25 3, . 5.8 25 1000 3. 5.9 , 0,01 . 5.10 , 0,1 . EN 14352 2013 4 5.11 , (). 5.12 () . 5.13 , , . 5.14 0,45 3. 5.15 : 5.15.1 , 1 3/. 5.15.2 , 20 3. 5.15.3 , - , -, (DS), , : ; 150 ; 4,6 ; 5 ; , - . 5.15.4 , , 335 440 , 1 2 0,5 - 3. 5.15.5 . 6 , , , - . 7 7.1 , 1 . - . 7.2 20 , 0,1 , 5.3. 50 3 4.14. , 20 5.2. 10 2500 g. 5.5, , . , 50 3. - 20 , 10 2500 g. , . 10,0 3 100 3. 40 3- 4.16,