1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS K ISO 2913 KS K ISO 2913:2009 2009 7 31 http:/www.kats.go.krKS K ISO 2913:2009 : ( ) ( ) LS FITI ( ) : (http:/www.standard.go.kr) ： ： 2004 11 25 ： 2009 7 31 2009-0374 ： ： ( 02-509-7278) (http:/www.kats.go.kr). 10 5 , . KS K ISO 2913:2009 i ii iii 1 1 2 1
2、3 1 4 2 5 .2 6 .3 7 .3 8 .4 9 .4 10 .5 A( ) .6 9 KS K ISO 2913:2009 ii . KS K ISO 2913： 2004 . . , , . , , . KS K ISO 2913:2009 iii 1975 1 ISO 2913, Wool Colorimetric determination of cystine plus cysteine in hydrolysates , . . . , , . . - (Folin-Shinohara) . , . (sodium disulfide) , (cysteine) 12 (
3、dodecatungstophosphoric acid) . . KS K ISO 2913:2009 Wool Colorimetric determination of cystine plus cysteine in hydrolysates 1 . , , , . , . , . , , . EDTA . 2 . . ( ) . KS L ISO 648, KS L ISO 1042, KS M 8113, ( ) ( ) KS M ISO 6353 2, 2： 1 KS M ISO 6353 3, 3： 2 3 . (sodium disulfide) 12- (dodecatun
4、gstophosphoric acid) , . . KS K ISO 2913:2009 2 . ( .) . a) , 1 % -5 (sodium pentacyanonitrosylferrate) . (red violet) . b) , (2 3) mL , -5 . . 4 . 4.1 , 6 N . KS M ISO 6353 2(R37) 1.84 g/mL, (95 98) % 150 mL 850 mL . 4.2 pH 5.6 , (2 ) 300 g, KS M ISO 63532(R1) 24 mL (II)5 KS M ISO 6353 2(R9) 1 mg 1
5、 L . 4.3 (tungstophosphoric acid reagent), 400 mL (2 , ) 200 g 85 % KS M ISO 6353 2(R22) 100 mL 1 . KS M ISO 6353 3(R51) . ( 15 ). 1 L . (yellow-brown) . 4.4 , (KS M 8113 ) 10 g 100 mL . 20 . 4.5 , 250 mL 100 mg (4.1) 20 mL . , . 5 5.1 , 0.2 mg . 5.2 , (ground glass) . 5.3 , (1052) . 5.4 5.5 , 100 m
6、L 25 mL( , KS L ISO 1042 , A B .) KS K ISO 2913:2009 3 5.6 , 15 mL, 10 mL, 5 mL, 2 mL 1 mL. 1 mL 5 mL , ( , KS L ISO 648 A .) . 5.7 ( ) 5.8 , ( ) 5.9 , 720 nm . 0 0.7 0.01 . 6 . 1 g 0.3 g , . 1 cm . . KS M ISO 63533(R57) 6 1 . . 7 7.1 6. 0.000 2 g . 1 g (7.2) 0.3 g (7.3). 7.2 (5.2) (5.3) (1052) . (5
7、.4) . .1) . . 7.3 100 mL (5.7) (4.1) 8 mL (1052) . , 0.5, 1, 1.5, 2, 2.5 . 10 , 100 mL (5.5) . 50 mL 1) 30 0.000 2 g . KS K ISO 2913:2009 4 (5.8). 7.4 7.4.1 10 mm , (720 890) nm . , . 10 mm . 0.70 . . 7.4.2 , . 7.4.3 (7.3) 5 mL 25 mL (5.5) (4.2) 15 mL (4.3) 2 mL . (20 30) . . 5 , A . 7.4.4 , (2 ) (7
8、.3) 1 mL 25 mL (5.5) (4.2) 5 mL, (4.4) 1 mL, (4.3) 2 mL . (20 30) . . B . 7.4.5 7.4.4 (4.5) 1 mL . C . C 0.56 0.70 . 8 S . mCABS=25)(100 m： (g) 9 . . KS K ISO 2913:2009 5 10 . a) b) c) d) , . e) KS K ISO 2913:2009 6 A ( ) A.1 . A.1 () 2004.11.25 2004 0893 ： ISO 2913 ： 1 2009.07.31 2009 0374 2008.8.2
9、5 ： a) disulphite disulfide b) 1. c) 1. d) 2. . ( ) , , . ( ) ( .) . . . ( ) . e) KS M 8113 f) KS M ISO 6353 2, KS M ISO 6353 3 KS K ISO 2913:2009 7 A.1 ( ) () 2009.07.31 2009 0374 g) ISO KS ISO 648 KS L ISO 648 ISO 1042 KS L ISO 1042 h) ( ) . 95 98 %(m/m) (95 98) % i) 4. KS M ISO 6353 2(R37) KS M I
10、SO 6353 2(R1) 24 mL ( )5 KSM ISO 6353 2(R9) 5 - (II) (II)5 KSM ISO 6353 2(R9) KS M ISO 6353 2(R22) KS M ISO 6353 3(R51) - (yellow-brown) (KS M 8113 ) j) 4., 5., 6., 7. , mass： , k) 5. ( .) ( ) l) 6. . . KS M ISO 6353 3(R57) m) 7. KS K ISO 2913:2009 8 A.1 ( ) () 2009.07.31 2009 0374 n) 10. , . o) p)
11、A KS K ISO 2913:2009 9 1 FOLIN AND LOONEY, J.Biol. Chemistry, 1922, 51, 421 2 FOLIN AND MARENZI, J. Biol. Chemistry, l929, 83, 103 3 FOLIN, J. Biol. Chemistry, 1934, 106, 311 4 LUGG, Biochem. J., 1932, 26, 2144, 2160 5 LUGG, Biochem. J., 1933, 27, 1022 6 SHINOHARA, J. Biol. Chemistry, 1935, 109, 665
12、 7 SHINOHARA, J. Biol. Chemistry, 1936, 112, 671, 683 8 SCHBERL AND RAMBACHER, Biochem. Z., 1938, 295, 377 9 SCHBERL, Textil-Praxis, 1959, 14, 701 10 BLACKBURN, Proc. Techn. Committee, Intern. Wool Textile Organiz., 1948, 2, 40 11 SPEAKMAN AND PARK, Bull. Inst. Textile France, 1952, 30, 255 12 ZAHN
13、AND TRAUMANN, Melliand Textilber, 1954, 35, 1069 13 T. GERTHSEN, I.W.T.O. Techn. Committee, May 1962, Report No. 12, and December 1962, Report No. 20 14 LEES AND ELSWORTH, I.W.T.O. Techn. Committee, May 1963, Report NO. 16 15 BAUTERS, LEFEBVRE AND VAN OVERBEKE, Bull. Inst. Textile France, 1964, 18, 773 135513 7017 (02)60094114 (02)600948878 http:/ KS K ISO 2913:2009 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS WoolColorimetric determination ofcystine plus cysteine in hydrolysates ICS 59.080.01 Korean Agency for Technology and Standards http:/www.kats.go.kr
