1、 2004 11 30 M ISO 10718 2004 ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( ) 2004 11 30 04 932 ( ) ( 02 509 7262 5) . 7 5 , ICS 07.100.99 55.100 79.100 KS M ISO 10718 2004 Cork stoppers Enumeration of colony-forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium 2002 2 ISO 10718 C
2、ork stoppers Enumeration of colony-forming units of yeasts, moulds and bacteria capable of growth in an alcoholic medium . , , . . . . KS M ISO 7218 ( , ) . (0.85 % NaCl)( 1 ) (1/4 X)( 1 ) . Sodium chloride Potassium chloride Calcium chloride 6H 2 O Sodium bicarbonate pH ( ) 2.25 g/L 0.105 g/L 12 g/
3、L 0.05 g/L 7.0 0.2 ( 1 ) . ( ) . M ISO 10718 2004 2 Yeast extract Casein hydrolysate Dextrose Potassium dihydrogen phosphate Magnesium chloride Calcium chloride Magnesium sulfate Manganese sulfate Ferric chloride Bromocresol green Cycloheximide (actidione) pH ( ) 4.0 g/L 5.0 g/L 50.0 g/L 0.55 g/L 0.
4、425 g/L 0.125 g/L 0.125 g/L 0.002 5 g/L 0.002 5 g/L 0.022 g/L 0.004 g/L 5.5 0.2 ( ) . Yeast extract Dextrose (cerelose) Peptone Magnesium sulfate Potassium phosphate Diastase Thiamine Bromocresol pH ( ) 9.0 g/L 50.0 g/L 10.0 g/L 2.10 g/L 2.0 g/L 0.05 g/L 0.05 g/L 0.026 g/L 4.6 0.2 96 % . . . 100 mL
5、, ( 0.45 m), 3 , . . 100mL (autoclave) , . , ( 0.45 m), (blotting pad) (petri) . 30 2 . 2 8 . 140 r/min 160 r/min . 140 60 . , 25 0.1 . , 4 100 mL M ISO 3869 2004 3 . . 2 8 . KS H ISO 7218 . ( ) . 10 g/L ( ) 1 g/L ( ) . , ( ) pH 3 3.5 . ( ) 90 mL . , 10 mL ( ) . , 4 . 140 r/min 160 r/min , 20 25 1 .
6、 . WLD M Green . , . . . ( ) . . . , . , 30 ppm WLD ( ) 10 % ( ) . (ampoule) WLD , . . . / . . . . , . M Green ( ) , . . . / . . . M ISO 10718 2004 4 WLD ( ) M Green ( ) . 30 ppm , WLD 10 % ( ) . . ( ) . . WLD . . . M Green . . . WLD M Green 30 2 ( ) 3 . 24 . , WLD . , (CFU) . 4 b N N b 4 , . , M Gr
7、een . , (CFU) . 4 m y N N y m 4 , . . M ISO 3869 2004 5 2004 12 26 135 513 701 7 (02)6009 4567 (02)6009 4887 8 http:/ 607 822 1 11( 10 ) (051)557 1239 Fax. (051)557 0430 702 012 2 1741( 3 ) (053)384 1562 4 Fax. (053)384 1565 790 380 627( ) (054)278 9611 Fax. (054)278 9662 406 130 994( 16 ) (032)260
8、0260 7 Fax. (032)260 0268 443 766 906 5( 9 ) (031)259 7000 9 Fax. (031)259 7010 200 041 1 9( 4 ) (033)252 9423, 254 9423 Fax. (033)256 9423 361 802 1508 1( 6 ) (043)236 2451 3 Fax. (043)236 2454 305 343 23 14( 5 ) (042)864 2301 3 Fax. (042)864 2304 561 841 1 337 2( 7 ) (063)214 2234 7 Fax. (063)214
9、2238 506 301 621 15( 4 ) (062)953 1435 7 Fax. (062)953 1438 641 740 7 4( 501 ) (055)266 4744 6 Fax. (055)266 4747 683 804 758 2( 2 ) (052)289 6601 3 Fax. (052)289 6604 425 852 773 9( 4 ) (031)492 5780, 8451 2 Fax. (031)492 8182 730 350 92 30( 8 ) (054)473 6954 Fax. (054)473 6955 480 848 2 487 6( 3 ) (031)829 8182 4 Fax. (031)829 8185 KSKSKS SKSKS KSKS SKS KSKS SKSKS KSKSKS 2004 ICS 07.100.99 55.100 79.100
