ASTM E1047-1985(2010) 4375 Standard Specification for Blood Sedimentation Tube Wintrobe Glass Disposable《易处置的温特罗布血沉玻璃试管的标准规范》.pdf

1、Designation: E1047 85 (Reapproved 2010)Standard Specification forBlood Sedimentation Tube, Wintrobe, Glass, Disposable1This standard is issued under the fixed designation E1047; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the y

2、ear of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers disposable blood sedimenta-tion tubes suitable for determining sedimentation rates an

3、d thevolume of packed red blood cells.1.2 The values stated in SI units are to be regarded asstandard. No other units of measurement are included in thisstandard.1.3 The following precautionary statement pertains only tothe test method portion, Section 7 of this specification. Thisstandard does not

4、purport to address all of the safety concerns,if any, associated with its use. It is the responsibility of the userof this standard to establish appropriate safety and healthpractices and determine the applicability of regulatory limita-tions prior to use.2. Referenced Documents2.1 ASTM Standards:2E

5、438 Specification for Glasses in Laboratory Apparatus3. Terminology3.1 Definitions of Terms Specific To This Standard:3.1.1 disposable, nin accordance with this specificationand the expected product performance expressed in this stan-dard, those tubes which are to be used one time only. Anyinstituti

6、on or individual who reuses a disposable tube must bearfull responsibility for its safety and effectiveness.3.1.2 Wintrobe3, nsurname of the individual responsiblefor the design of the reusable Wintrobe tube and the method ofuse for both reusable and disposable tubes.4. Classification4.1 This specif

7、ication covers a tube that is intended to beused one time only. It is not to be confused with a reusable tubethat is described in other published standards.5. Materials5.1 GlassThe tubes made to this specification shall befabricated borosilicate glass, Type I, Class B, or from soda limeglass, Type I

8、I, in accordance with Specification E438.6. Physical Requirements6.1 DesignThe tube shall be made of tubing with aminimum wall thickness of 0.6 mm. The tube shall have acylindrical body terminating in a round bottom on the outsideand a flat bottom on the inside. The tube shall have a uniformbore and

9、 a fire-polished top.6.2 WorkmanshipThe tube shall be free as possible fromvisible defects which detract from its serviceability. The tubeshall be well annealed so there will be no ring strain evidentwhen tested under a polariscope.6.3 DimensionsThe tube shall be made of tubing with anoutside diamet

10、er (O.D.) of 5.0 6 0.5 mm and an insidediameter (I.D.) of 2.9 to 3.5 mm. The uniformity of the boreshall be 60.15 mm throughout the tube. The tube shall be 110to 117 mm long and have a graduated scale of 100 mm.Manufacturers have the option to place additional graduationlines on the tube to a maximu

11、m of 105 mm. Maximum overalltolerance on the total graduation scale shall be 61.0 mm. Thetube shall be legibly marked with the makers or vendorsname or mark and possess a wide color band at the top of thetube for marking purposes.6.4 Graduation ScaleThe tube shall be graduated 100 to105 6 1.0 mm in

12、1 mm divisions and numbered everycentimetre with two sets of numerals. One set of graduationnumerals shall be from 0 to 9 cm down the left side of thegraduation scale and the other set of graduation numerals shallbefrom1to10cmup the right side of the graduation scale asshown in Fig. 1.6.5 Graduation

13、 LinesThe thickness of the graduationlines may vary in uniformity but shall not exceed 0.4 mm inthickness. They shall lie at right angles to the axis of the tubewith a maximum of 0.2 mm tolerance between two adjacentlines. Every tenth (numbered) line shall be a minimum of5 mm. The medium (every fift

14、h) line shall be a minimum of3 mm. The short (intermediate) lines shall be a minimum of2 mm.6.6 Resistance to Centrifugal ForceThe tube shall notchip, crack, or break when tested as specified in 7.2.1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the

15、 direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved July 1, 2010. Published July 2010. Originally approvedin 1985. Last previous edition approved in 2005 as E1047 85 (2005). DOI:10.1520/E1047-85R10.2For referenced ASTM standards, visit the ASTM website, www.astm.org, o

16、rcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.3Wintrobe, M. M., “Laboratory Evaluation of Erythrocytes,” Clinical Hema-tology, Seventh E., 1974, pp. 109134.1Copyright ASTM Intern

17、ational, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.6.7 Marking PermanencyInscriptions, graduation lines,and numerals may be printed on the tube in black, blue, green,or white coloring. The markings may not be of permanentnature but must possess sufficient st

18、ability to endure normaltransportation and its expected one time use and meet therequirements as specified in 7.1.7. Test Methods7.1 Marking Permanency TestImmerse tube in water for30 s. Using a soft paper tissue, wipe the graduated portion ofthe wet tube lightly with ten complete strokes (five up a

19、nd fivedown). When judged by the eye under normal room lightingthe tube markings should appear as before the test with onlypossible fading of the printed decorations, or minor removal ofmarkings that would not effect the tubes serviceability, or both.7.2 Resistance to Centrifugal Force TestThe tube

20、shall befilled with water to the top graduation line and placed in acentrifuge with a horizontal centrifuge head. Speed and dimen-sions of the centrifuge head shall be such that the inside bottomof the tube is subjected to a relative centrifugal force (RCF) notless than 2500 g. The formula for calcu

21、lating relative centrifu-gal force is as follows:RCF = = relative centrifugal force (g)RCF = = 00001118 3 r 3 N2r= = rotating radius to bottom of tube (cm)N= = rotating speed (r/min)8. Keywords8.1 disposable; glass; tube; WintrobeFIG. 1 Wintrobe TubeE1047 85 (2010)2ASTM International takes no positi

22、on respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility

23、.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM

24、International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address sh

25、own below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org). Permission rights to photocopy the standard may also be secured from the ASTM website (www.astm.org/COPYRIGHT/).E1047 85 (2010)3