BS ISO 5061-2002 Animal feeding stuffs - Determination of castor oil seed husks - Microscope method《动物饲料 蓖麻油种子壳的测定 显微镜法》.pdf

1、BRITISH STANDARD BS ISO 5061:2002 Animal feeding stuffs Determination of castor oil seed husks Microscope method ICS 65.120 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS ISO 5061:2002 This British Standard, having been prepared under the direction of the Consumer Products

2、and Services Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 18 June 2002 BSI 18 June 2002 ISBN 0 580 39806 4 National foreword This British Standard reproduces verbatim ISO 5061:2002 and implements it as the UK national stand

3、ard. It supersedes BS 5766:Part 9:1983 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee AW/10, Animal feeding stuffs, which has the responsibility to: A list of organizations represented on this subcommittee can be obtained on request to its secretary.

4、 Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or British St

5、andards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to t

6、he responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside f

7、ront cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7, and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsINTERNATIONAL STANDARD ISO 5061 Second edition 2002

8、-04-15 Reference number ISO 5061:2002(E) Animal feeding stuffs Determination of castor oil seed husks Microscope method Aliments des animaux Dtermination des coques de graines de ricin Mthode au microscopeISO 5061:2002(E) ii FDP dicslaimre This FDP fam elitnoc yt deddebme niayfep.seca In roccatiw ec

9、nadA hycilop gnisnecil sebod, thf sim eliarp eb ytnieiv ro dewb detu sllah ton eb tidesselnu de tt ehyfepseca hwich rae meil era deddebct desneo tsni dnalat no delmoc ehtupre epfrromign tide ehti.gn In odaolnwding htis life, rapticca setpe trehiet neh serilibisnopty fo ton fnirisebodA gnign ilop gni

10、snecilcy. TI ehSO tneCraceS lrteraiat catpecon s illibayti in ihts aera. ebodA si t armedarak fo dAS ebotsysme Inroctarop.de teDailfo s tfos ehtware prtcudos t desuo crtaet ehFDP si ff eb nac elit ni dnuoG ehlarene Info talerive to tf eh;eli teh PDF-rctaeirap nomateers weer tpof dezimior rptnii.gn v

11、Erec yaeeb sah ert nakt neo rusnet etah tf ehi elis tiusbaf elob esu ry ISO merebm seidob. In the kilnuley evtne ttah a borpmel talerign to ti f si,dnuo saelpfni eorm teh tneCS laretercratai ta thserdda es igvne olebw. ISO 2002 Ar llieser sthgrvsselnU .de torehceps esiwfi,dei rap ont iht fos bupliac

12、tim noarper eb yro decudo tuzilina ni dey form b roy nasnaem y, tcelernahcem ro cinoicla, inclidung tohppocoying m dnaicrfomli, tiwtuoh permini noiss wrtif gnirmo ietI rehSO ta tserdda eheb slow or SIsO memreb ydob in toc ehtnury fo tr ehtseuqeer. ISO copyrithg fofiec tsop esaCael 65 G 1121-HCeneva

13、02 Te.l + 22 14 10 947 11 Fax + 14 47 2290 9 74 E-liam copyrightsio.ch Web wwwsi.oc.h Prni detni SztiwerldnaISO 5061:2002(E)iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing Internat

14、ional Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with I

15、SO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standard

16、s adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be

17、 the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 5061 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs. This second edition cancels and replaces the first

18、edition (ISO 5061:1983), of which it constitutes a minor revision. The specification for magnification has been changed.INTERNATIONAL STANDARD ISO 5061:2002(E)1 Animal feeding stuffs Determination of castor oil seed husks Microscope method 1 Scope This International Standard specifies a method for t

19、he determination of castor oil seed (Ricinus communis) husks in single and compound animal feeding stuffs and, in particular, in oilseed residues. The limits of detection is . The method requires final microscopical identification of the isolated husks. This last phase therefore requires a specialis

20、t who has had sufficient experience in this type of identification and who is experienced in microscopic techniques. 2 Principle A test portion is boiled successively with nitric acid and sodium hydroxide solution. After washing, the residue is separated by decanting. The fragments of castor oil see

21、d husks are dried, identified using a microscope, then weighed. 3R e a g e n t s Use only reagents of recognized analytical grade and distilled or demineralized water or water of equivalent purity. 3.1 Nitric acid, (by volume) solution. 3.2 Sodium hydroxide, solution. 4A p p a r a t u s Usual labora

22、tory equipment and, in particular, the following. 4.1 Stereomicroscope or binocular lens, of total magnification to . 4.2 Microscope and accessories, of magnification to . 4.3 Oven, capable of being maintained at . 4.4 Nylon gauze, of mesh size , resistant to dilute acids and alkalis. 4.5 Sieve, of

23、aperture size . 4.6 Porcelain dish, of capacity to . 4.7 Measuring cylinder, of capacity at least . 4.8 Flat-bottomed dish, approximately . 4.9 Desiccator. 5 mg/kg 10 % 25 g/l 5 50 100 1 000 103 C 2 C 100 m 3mm 1 000 ml 2 000 ml 1 000 ml 140 mm 80 mmISO 5061:2002(E) 2 4.10 Analytical balance. 5 Samp

24、ling It is important the laboratory receive a sample which is truly representative and has not been damaged or changed during transport or storage. Sampling is not part of the method specified in this International Standard. A recommended sampling method is given in ISO 6497. 1) 6 Procedure 6.1 Prep

25、aration of test sample 6.1.1 Powdered feeding stuffs Thoroughly mix the laboratory sample. 6.1.2 Cakes or compressed feeding stuffs Grind the laboratory sample coarsely so that it passes completely through the sieve (4.5). Mix well after sieving. 6.2 Test portion Weigh, to the nearest , about of the

26、 test sample into the porcelain dish (4.6). 6.3 Determination 6.3.1 Add to of the nitric acid (3.1). Bring to the boil, stirring continuously with a glass rod, and allow to boil for half a minute. Filter through the nylon gauze (4.4). Wash the residue with hot water and transfer back to the porcelai

27、n dish. Add to of the sodium hydroxide solution (3.2). Bring to the boil, stirring continuously with a glass rod, and allow to boil for half a minute. Transfer the suspension to the measuring cylinder (4.7) and fill the measuring cylinder with water. 6.3.2 Pass a slight flow of water through the mea

28、suring cylinder by means of a glass tube immersed to a depth of two-thirds of the height of the measuring cylinder. Adjust the flow so that only the very finest particles remain in suspension and the husk fragments remain at the bottom. Continue this operation until the majority of the particles in

29、suspension have been removed. Decant two-thirds of the liquid and filter the remainder through the nylon gauze (4.4). 6.3.3 T ransfer the residue to the flat-bottomed dish (4.8). Examine under the stereomicroscope or the binocular lens (4.1) and isolate the husk fragments on a white background using

30、 tweezers. Dry for in the oven (4.3) set at . Allow to cool to ambient temperature in the desiccator and identify the fragments using the microscope (4.2) by comparing them with castor oil seed husks which have been subjected to the same treatment. 6.3.4 Castor oil seed husks have a particular struc

31、ture; the black or brown, acutely angled, husk fragments have a characteristic pitted surface which can be seen when examined under low magnification (see Figures 1 to 7). Collect the husks and weigh them to the nearest . 1) ISO 6497, Animal feeding stuffs Sampling. 0,1 g 100 g 500 ml 700 ml 500 ml

32、700 ml 4h 103 C 0,1 mgISO 5061:2002(E)3 6.4 Number of determinations Carry out three determinations on test portions taken from the same test sample. 7 Expression of results The castor oil seed husks content, , expressed in milligrams per kilogram of the product as received, is equal to where is the

33、 mass, in grams, of the test portion; is the mass, in milligrams, of dried castor oil seed husk fragments; 1,3 is a factor used to correct for the estimated loss of in the three steps of the analysis (6.3). Express the result to the nearest unit as the arithmetic mean of the three determinations. 8

34、Test report The test report shall specify: all information necessary for the complete identification of the sample; the sampling method used, if known; the test method used, with reference to this International Standard; all operating details not specified in this International Standard, or regarded

35、 as optional, together with details of any incidents which may have influenced the test result; the test result obtained. w w=m 1 1,3 1 000 m 0 m 0 m 1 30 %ISO 5061:2002(E) 4 Key 1E p i d e r m i s 2F i b r e s Figure 1 Ricinus communis Cross section of testa (magnification: ) Figure 2 Ricinus commu

36、nis Epidermal cells of testa (magnification: ) 400 300ISO 5061:2002(E)5 Figure 3 Ricinus communis Epidermal cells of testa (magnification: ) Figure 4 Ricinus communis Epidermal cells of testa (magnification: ) 500 800ISO 5061:2002(E) 6 Figure 5 Ricinus communis Testa fibres (magnification: ) Figure

37、6 Ricinus communis Testa fibres (magnification: ) 200 300ISO 5061:2002(E)7 Dimensions in millimetres Figure 7 Ricinus communis Testa fibresBS ISO 5061:2002 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing Britis

38、h Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It is th

39、e constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cov

40、er. Tel: +44 (0)20 8996 9000. Fax: +44 (0)20 8996 7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be

41、 addressed to Customer Services. Tel: +44 (0)20 8996 9001. Fax: +44 (0)20 8996 7001. Email: ordersbsi-. Standards are also available from the BSI website at http:/www.bsi-. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been pu

42、blished as British Standards, unless otherwise requested. Information on standards BSI provides a wide range of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also availabl

43、e which give details on all its products and services. Contact the Information Centre. Tel: +44 (0)20 8996 7111. Fax: +44 (0)20 8996 7048. Email: infobsi-. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards.

44、 For details of these and other benefits contact Membership Administration. Tel: +44 (0)20 8996 7002. Fax: +44 (0)20 8996 7001. Email: membershipbsi-. Information regarding online access to British Standards via British Standards Online can be found at http:/www.bsi- Further information about BSI is

45、 available on the BSI website at http:/www.bsi-. Copyright Copyright subsists in all BSI publications. BSI also holds the copyright, in the UK, of the publications of the international standardization bodies. Except as permitted under the Copyright, Designs and Patents Act 1988 no extract may be rep

46、roduced, stored in a retrieval system or transmitted in any form or by any means electronic, photocopying, recording or otherwise without prior written permission from BSI. This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size

47、, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained. Details and advice can be obtained from the Copyright & Licensing Manager. Tel: +44 (0)20 8996 7070. Fax: +44 (0)20 8996 7553. Email: copyrightbsi-.