1、BRITISH STANDARD BS ISO 5061:2002 Animal feeding stuffs Determination of castor oil seed husks Microscope method ICS 65.120 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBS ISO 5061:2002 This British Standard, having been prepared under the direction of the Consumer Products
2、and Services Sector Policy and Strategy Committee, was published under the authority of the Standards Policy and Strategy Committee on 18 June 2002 BSI 18 June 2002 ISBN 0 580 39806 4 National foreword This British Standard reproduces verbatim ISO 5061:2002 and implements it as the UK national stand
3、ard. It supersedes BS 5766:Part 9:1983 which is withdrawn. The UK participation in its preparation was entrusted to Technical Committee AW/10, Animal feeding stuffs, which has the responsibility to: A list of organizations represented on this subcommittee can be obtained on request to its secretary.
4、 Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or British St
5、andards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to t
6、he responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document comprises a front cover, an inside f
7、ront cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7, and a back cover. The BSI copyright date displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date CommentsINTERNATIONAL STANDARD ISO 5061 Second edition 2002
8、-04-15 Reference number ISO 5061:2002(E) Animal feeding stuffs Determination of castor oil seed husks Microscope method Aliments des animaux Dtermination des coques de graines de ricin Mthode au microscopeISO 5061:2002(E) ii FDP dicslaimre This FDP fam elitnoc yt deddebme niayfep.seca In roccatiw ec
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13、02 Te.l + 22 14 10 947 11 Fax + 14 47 2290 9 74 E-liam copyrightsio.ch Web wwwsi.oc.h Prni detni SztiwerldnaISO 5061:2002(E)iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing Internat
14、ional Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with I
15、SO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3. Draft International Standard
16、s adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this International Standard may be
17、 the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. International Standard ISO 5061 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 10, Animal feeding stuffs. This second edition cancels and replaces the first
18、edition (ISO 5061:1983), of which it constitutes a minor revision. The specification for magnification has been changed.INTERNATIONAL STANDARD ISO 5061:2002(E)1 Animal feeding stuffs Determination of castor oil seed husks Microscope method 1 Scope This International Standard specifies a method for t
19、he determination of castor oil seed (Ricinus communis) husks in single and compound animal feeding stuffs and, in particular, in oilseed residues. The limits of detection is . The method requires final microscopical identification of the isolated husks. This last phase therefore requires a specialis
20、t who has had sufficient experience in this type of identification and who is experienced in microscopic techniques. 2 Principle A test portion is boiled successively with nitric acid and sodium hydroxide solution. After washing, the residue is separated by decanting. The fragments of castor oil see
21、d husks are dried, identified using a microscope, then weighed. 3R e a g e n t s Use only reagents of recognized analytical grade and distilled or demineralized water or water of equivalent purity. 3.1 Nitric acid, (by volume) solution. 3.2 Sodium hydroxide, solution. 4A p p a r a t u s Usual labora
22、tory equipment and, in particular, the following. 4.1 Stereomicroscope or binocular lens, of total magnification to . 4.2 Microscope and accessories, of magnification to . 4.3 Oven, capable of being maintained at . 4.4 Nylon gauze, of mesh size , resistant to dilute acids and alkalis. 4.5 Sieve, of
23、aperture size . 4.6 Porcelain dish, of capacity to . 4.7 Measuring cylinder, of capacity at least . 4.8 Flat-bottomed dish, approximately . 4.9 Desiccator. 5 mg/kg 10 % 25 g/l 5 50 100 1 000 103 C 2 C 100 m 3mm 1 000 ml 2 000 ml 1 000 ml 140 mm 80 mmISO 5061:2002(E) 2 4.10 Analytical balance. 5 Samp
24、ling It is important the laboratory receive a sample which is truly representative and has not been damaged or changed during transport or storage. Sampling is not part of the method specified in this International Standard. A recommended sampling method is given in ISO 6497. 1) 6 Procedure 6.1 Prep
25、aration of test sample 6.1.1 Powdered feeding stuffs Thoroughly mix the laboratory sample. 6.1.2 Cakes or compressed feeding stuffs Grind the laboratory sample coarsely so that it passes completely through the sieve (4.5). Mix well after sieving. 6.2 Test portion Weigh, to the nearest , about of the
26、 test sample into the porcelain dish (4.6). 6.3 Determination 6.3.1 Add to of the nitric acid (3.1). Bring to the boil, stirring continuously with a glass rod, and allow to boil for half a minute. Filter through the nylon gauze (4.4). Wash the residue with hot water and transfer back to the porcelai
27、n dish. Add to of the sodium hydroxide solution (3.2). Bring to the boil, stirring continuously with a glass rod, and allow to boil for half a minute. Transfer the suspension to the measuring cylinder (4.7) and fill the measuring cylinder with water. 6.3.2 Pass a slight flow of water through the mea
28、suring cylinder by means of a glass tube immersed to a depth of two-thirds of the height of the measuring cylinder. Adjust the flow so that only the very finest particles remain in suspension and the husk fragments remain at the bottom. Continue this operation until the majority of the particles in
29、suspension have been removed. Decant two-thirds of the liquid and filter the remainder through the nylon gauze (4.4). 6.3.3 T ransfer the residue to the flat-bottomed dish (4.8). Examine under the stereomicroscope or the binocular lens (4.1) and isolate the husk fragments on a white background using
30、 tweezers. Dry for in the oven (4.3) set at . Allow to cool to ambient temperature in the desiccator and identify the fragments using the microscope (4.2) by comparing them with castor oil seed husks which have been subjected to the same treatment. 6.3.4 Castor oil seed husks have a particular struc
31、ture; the black or brown, acutely angled, husk fragments have a characteristic pitted surface which can be seen when examined under low magnification (see Figures 1 to 7). Collect the husks and weigh them to the nearest . 1) ISO 6497, Animal feeding stuffs Sampling. 0,1 g 100 g 500 ml 700 ml 500 ml
32、700 ml 4h 103 C 0,1 mgISO 5061:2002(E)3 6.4 Number of determinations Carry out three determinations on test portions taken from the same test sample. 7 Expression of results The castor oil seed husks content, , expressed in milligrams per kilogram of the product as received, is equal to where is the
33、 mass, in grams, of the test portion; is the mass, in milligrams, of dried castor oil seed husk fragments; 1,3 is a factor used to correct for the estimated loss of in the three steps of the analysis (6.3). Express the result to the nearest unit as the arithmetic mean of the three determinations. 8
34、Test report The test report shall specify: all information necessary for the complete identification of the sample; the sampling method used, if known; the test method used, with reference to this International Standard; all operating details not specified in this International Standard, or regarded
35、 as optional, together with details of any incidents which may have influenced the test result; the test result obtained. w w=m 1 1,3 1 000 m 0 m 0 m 1 30 %ISO 5061:2002(E) 4 Key 1E p i d e r m i s 2F i b r e s Figure 1 Ricinus communis Cross section of testa (magnification: ) Figure 2 Ricinus commu
36、nis Epidermal cells of testa (magnification: ) 400 300ISO 5061:2002(E)5 Figure 3 Ricinus communis Epidermal cells of testa (magnification: ) Figure 4 Ricinus communis Epidermal cells of testa (magnification: ) 500 800ISO 5061:2002(E) 6 Figure 5 Ricinus communis Testa fibres (magnification: ) Figure
37、6 Ricinus communis Testa fibres (magnification: ) 200 300ISO 5061:2002(E)7 Dimensions in millimetres Figure 7 Ricinus communis Testa fibresBS ISO 5061:2002 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing Britis
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