1、December 2014 Translation by DIN-Sprachendienst.English price group 16No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).I
2、CS 13.040.20!%Zp“2275577www.din.deDDIN EN ISO 16000-19Indoor air Part 19: Sampling strategy for moulds (ISO 16000-19:2012);English version EN ISO 16000-19:2014,English translation of DIN EN ISO 16000-19:2014-12Innenraumluftverunreinigungen Teil 19: Probenahmestrategie fr Schimmelpilze (ISO 16000-19:
3、2012);Englische Fassung EN ISO 16000-19:2014,Englische bersetzung von DIN EN ISO 16000-19:2014-12Air intrieur Partie 19: Stratgie dchantillonnage des moisissures (ISO 16000-19:2012);Version anglaise EN ISO 16000-19:2014,Traduction anglaise de DIN EN ISO 16000-19:2014-12SupersedesDIN ISO 16000-19:201
4、2-12www.beuth.deIn case of doubt, the German-language original shall be considered authoritative.Document comprises 40 pages12.14This standard has been included in the VDI/DIN Handbook on air quality, volumes 1a and 5.DIN EN ISO 16000-19:2014-12 2 A comma is used as the decimal marker. National fore
5、word International technical regulation pertaining to indoor air This standard has been prepared by Technical Committee ISO/TC 146 “Air quality”, Subcommittee SC 6 “Indoor air”, Working Group WG 10 “Fungi” (Convenership and Secretariat: DIN, Germany). The responsible German body involved in its prep
6、aration was the Kommission Reinhaltung der Luft (KRdL) im VDI und DIN Normenausschuss (Commission on Air Pollution Prevention of VDI and DIN Standards Committee), Joint Committee NA 134-03-07 AA Bioaerosole und biologische Agenzien. The sampling strategy described in this standard is based on VDI 43
7、00 Part 10. Thus, this strategy is not only widely used in Germany, but throughout the world. This means that those using this strategy do not need to modify their procedures and previous results do not need to be converted, which could be difficult and would lead to errors. The measurement strategy
8、 described in VDI 4300 Part 10 is identical to that described here, except for references to German laws and regulations. Sampling, which together with cultivation forms part of the overall measurement procedure, is described in DIN ISO 16000-16 (filtration method) and in DIN ISO 16000-18 (impaction
9、 method). The cultivation method is specified in ISO 16000-17. These three methods are also based on VDI Guidelines, namely VDI 4252 Part 2 and VDI 4253 Part 2, which deal with air measurement and were validated in extensive comparative studies, and also VDI 4300 Part 10. General and substance-speci
10、fic aspects which should be taken into account before or during measurements, as well as methods for determining individual substances or substance groups, and microorganisms in indoor air are described in the various parts of ISO 16000. Attention is drawn to the possibility that some of the element
11、s of this document may be the subject of patent rights. DIN shall not be held responsible for identifying any or all such patent rights. The DIN Standards corresponding to the International Standards referred to in Clause 2 of this standard are as follows: ISO 16000-16 DIN ISO 16000-16 ISO 16000-18
12、DIN ISO 16000-18 National technical regulation pertaining to indoor air The Robert Koch Institute (RKI) has published recommendations for carrying the health risk assessment of mould growth in indoor environments (see 1 National Annex NA “Bibliography”). General conditions and other aspects to be ta
13、ken into consideration when planning indoor air measurements are described for individual substances and substance groups in the VDI 4300 series (see also “National Annex NA”). Some of these served as the sole basis for parts of the ISO 16000 series (e.g. ISO 16000-1, ISO 16000-2, ISO 16000-5, ISO 1
14、6000-7, ISO 16000-12, ISO 16000-15, ISO 16000-16, ISO 16000-17, ISO 16000-18, ISO 16000-26 and ISO 16000-32). The specific procedures (sampling, analysis) are described in the VDI 4301 series for individual substances and substance groups. Methods which can be used for both indoor and ambient air me
15、asurements are described in further VDI Guidelines. DIN EN ISO 16000-19:2014-12 3 The DIN ISO 16000 series and DIN EN ISO 16000 series consist of the following parts, under the general title Indoor air: DIN EN ISO 16000-1, General aspects of sampling strategy DIN EN ISO 16000-2, Sampling strategy fo
16、r formaldehyde DIN ISO 16000-3, Determination of formaldehyde and other carbonyl compounds in indoor air and test chamber air Active sampling method DIN ISO 16000-4, Determination of formaldehyde Diffusive sampling method DIN EN ISO 16000-5, Sampling strategy for volatile organic compounds (VOCs) DI
17、N ISO 16000-6, Determination of volatile organic compounds in indoor and test chamber air by active sampling on Tenax TA sorbent, thermal desorption and gas chromatography using MS/FID DIN EN ISO 16000-7, Sampling strategy for determination of airborne asbestos fibre concentrations DIN ISO 16000-8,
18、Determination of local mean ages of air in buildings for characterizing ventilation condi-tions DIN EN ISO 16000-9, Determination of the emission of volatile organic compounds from building products and furnishing Emission test chamber method DIN EN ISO 16000-10, Determination of the emission of vol
19、atile organic compounds from building pro-ducts and furnishing Emission test cell method DIN EN ISO 16000-11, Determination of the emission of volatile organic compounds from building pro-ducts and furnishing Sampling, storage of samples and preparation of test specimens DIN EN ISO 16000-12, Samplin
20、g strategy for polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polycyclic aromatic hydrocarbons (PAHs) DIN ISO 16000-13, Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorin
21、ated dibenzo-p- dioxins/dibenzofurans (PCDDs/PCDFs) Collection on sorbent-backed filters DIN ISO 16000-14, Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/PCDFs) Extraction, clean-up and analysis
22、by high-resolution gas chromatography/mass spectrometry DIN EN ISO 16000-15, Sampling strategy for nitrogen dioxide (NO2) DIN ISO 16000-16, Detection and enumeration of moulds Sampling by filtration DIN ISO 16000-17, Detection and enumeration of moulds Culture-based method DIN ISO 16000-18, Detectio
23、n and enumeration of moulds Sampling by impaction DIN EN ISO 16000-19, Sampling strategy for moulds DIN ISO 16000-21, Detection and enumeration of moulds Sampling from materials DIN ISO 16000-23, Performance test for evaluating the reduction of formaldehyde concentrations by sorptive building materi
24、als DIN EN ISO 16000-19:2014-12 4 DIN ISO 16000-24, Performance test for evaluating the reduction of volatile organic compounds and carbonyl compounds without formaldehyde concentrations by sorptive building materials DIN ISO 16000-25, Determination of the emission of semi-volatile organic compounds
25、 by building pro-ducts Micro-chamber method DIN EN ISO 16000-26,Sampling strategy for carbon dioxide (CO2) DIN ISO 16000-27, Standard method for the quantitative analysis of asbestos fibres in settled dust DIN ISO 16000-28, Determination of odour emissions from building products using test chambers
26、DIN ISO 16000-29, Test methods for VOC detectors DIN ISO 16000-30, Sensory testing of indoor air DIN ISO 16000-31, Measurement of flame retardants and plasticizers based on organophosphorus compounds Phosphoric acid ester DIN ISO 16000-32, Investigation of constructions on pollutants and other injur
27、ious factors Inspections The following part is under preparation: DIN ISO 16000-20, Detection and enumeration of moulds Determination of total spore count The following part is planned: DIN ISO 16000-22, Detection and enumeration of moulds Molecular methods Amendments This standard differs from DIN
28、ISO 16000-19:2012-12 as follows: a) the text of ISO 16000-19:2012 has been adopted by CEN as EN ISO 16000-19 without any modifications. Previous editions DIN ISO 16000-19: 2012-12 DIN EN ISO 16000-19:2014-12 5 National Annex NA (informative) Bibliography Schimmelpilzbelastung in Innenrumen Befunderh
29、ebung, gesundheitliche Bewertung und Manahmen; Mit-teilung der Kommission “Methoden und Qualittssicherung in der Umweltmedizin” des Robert Koch Instituts (2007). Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 50:1308-1323. DIN EN ISO 5492, Sensory analysis Vocabulary DIN EN ISO 8589,
30、Sensory analysis General guidance for the design of test rooms DIN 50014, Climates and their technical application Standard atmospheres DIN EN ISO 8199, Water quality General guidance on the enumeration of microorganisms by culture DIN EN ISO 9169, Air quality Definition and determination of perform
31、ance characteristics of an automatic measuring system DIN ISO 12219-1, Indoor air of road vehicles Part 1: Whole vehicle test chamber Specification and method for the determination of volatile organic compounds in cabin interiors DIN ISO 12219-2, Indoor air of road vehicles Part 2: Screening method
32、for the determination of the emis-sions of volatile organic compounds from vehicle interior parts and materials Bag method DIN ISO 12219-3, Indoor air of road vehicles Part 3: Screening method for the determination of the emis-sions of volatile organic compounds from vehicle interior parts and mater
33、ials Micro-scale chamber method DIN ISO 12219-4, Indoor air of road vehicles Part 4: Screening method for the determination of the emis-sions of volatile organic compounds from vehicle interior parts and materials Small chamber method DIN ISO 12219-5, Indoor air of road vehicles Part 5: Screening me
34、thod for the determination of the emis-sions of volatile organic compounds from vehicle interior parts and materials Static chamber method DIN ISO 12884, Ambient air Determination of total (gas and particle phase) polycyclic aromatic hydrocar-bons Collection on sorbent-backed filters with gas chroma
35、tographic/mass spectrometric analysis DIN V ENV 13005, Guide to the expression of uncertainty in measurement DIN EN 14412, Indoor air quality Diffusive samplers for the determination of concentrations of gases and vapours Guide for selection, use and maintenance DIN EN ISO 16017-1, Indoor, ambient a
36、nd workplace air Sampling and analysis of volatile organic com-pounds by sorbent tube/thermal desorption/capillary gas chromatography Part 1: Pumped sampling DIN EN ISO 16017-2, Indoor, ambient and workplace air Sampling and analysis of volatile organic compounds by sorbent tube/thermal desorption/c
37、apillary gas chromatography Part 2: Diffusive sampling DIN ISO 16362, Ambient air Determination of particle-phase polycyclic aromatic hydrocarbons by high per-formance liquid chromatography DIN EN ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories DIN EN I
38、SO 16000-19:2014-12 6 VDI 2464 Part 1, Ambient air measurement Indoor air measurement Measurement of polychlorinated biphenyls (PCBs) GC/MS method for PCB 28, 52, 101, 138, 153, 180 VDI 2464 Part 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated biphenyls (PCBs) HR-GC/
39、HR-MS method for coplanar PCBs VDI 2464 Part 3, Ambient air measurement Indoor air measurement Measurement of polybrominated diphenylether, hexabromocyclododecane and hexabromobenzene by GC/MS VDI 3484 Part 1, Gaseous ambient air measurements Indoor-air pollution measurements Test gases measurements
40、 Measurement of the formaldehyde concentration with the sulfite pararosaniline method VDI 3484 Part 2, Gaseous ambient air measurements Indoor-air pollution measurements Measurement of the formaldehyde concentration with the acetylacetone method VDI 3498 Part 1, Ambient air measurement Indoor air me
41、asurement Measurement of polychlorinated dibenzo-p-dioxins and dibenzofurans Method using large filters VDI 3498 Part 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated dibenzo-p-dioxins and dibenzofurans Method using small filters VDI 4252 Part 2, Measurement of airbor
42、ne microorganisms and viruses in ambient air Active sampling of bioaerosols Separation of airborne mould on gelatine/polycarbonate filters VDI 4253 Part 2, Measurement of airborne microorganisms and viruses in ambient air Culture based method for the determination of the concentration of mould in ai
43、r Indirect method after sampling with gelatine/polycarbonate filters VDI 4300 Part 1, Indoor-air pollution measurement General aspects of measurement strategy VDI 4300 Part 2, Indoor-air pollution measurement Measurement strategy for polycyclic aromatic hydro-carbons (PAHs), polychlorinated dibenzo-
44、p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) VDI 4300 Part 4, Indoor-air pollution measurement Measurement strategy for pentachlorophenol (PCP) and -hexachlorocyclohexane (lindane) in indoor air VDI 4300 Part 5, Indoor-air pollution measurement Measur
45、ement strategy for nitrogen dioxide (NO2) VDI 4300 Part 6, Measurement of indoor air pollution Measurement strategy for volatile organic compounds (VOCs) VDI 4300 Part 7, Indoor-air pollution measurement Measurement of indoor air change rate VDI 4300 Part 8, Measurement of indoor air pollution Sampl
46、ing of house dust VDI 4300 Part 9, Measurement of indoor air pollution Measurement strategy for carbon dioxide (CO2) VDI 4300 Part 10, Measurement of indoor air pollution Measurement strategies for determination of mould fungi in indoor environment VDI 4300 Part 11, Measurement of indoor air polluti
47、on Measurement strategies for determination of air-borne particles in indoor environment Particles with equivalent diameter 30 min) are appropriate methods. In practice, sampling by impaction is the preferred procedure for short-term sampling. This sampling method requires a prior estimate of the ex
48、pected mould concentration. Different air volumes are sampled at each sampling location to be able to cover a broader concentration range. This is accomplished by collecting impaction samples over different sampling durations. The detection of indoor-relevant moulds presupposes that particles with a
49、 diameter greater than 2 m can be quantitatively collected on the culture medium or adhesive-coated slide (for the determination of the total spore count). This presupposes that the impactors are designed for a cut-off d50 2 m (see Table B.2). All short-term measurements should be conducted over a minimum period of 1 min. The sample volume should not be les
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