GOST 31709-2012 Milk and milk powder Determination of aflatoxin M1 content Clean-up by immunoaffinity chromatography and determination by thin-layer chromatography《奶和奶粉 黄曲霉毒素M1含量测定.pdf

1、 1. (ISO 14674:2005, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)317092012(ISO 14674:2005), - 1.092 . - 1.22009 . , . , , -, 1 - ( ) , - 42 3 , (- 1 2012 . 51) : ( 3166) 00497 ( 3166) 00497 BYKZKGMDRUTJ -4 29 2012.1772-317092012(ISO14674:2005) 1 2013 .5 ISO14674:

2、2005 Milk and milk powder. Determination of aflatoxin M1content. Clean-up by immunoaffinitychromatography and determination by thin-layer chromatography ( . 1. ). , , , - , -. - . (MOD). 5283120076 ( ) - . , - . - , 2013 -, II 3170920121 12 .13 24 .25 26 37 .48 48.1 .48.2 .48.3 59 .59.1 .59.2 510 61

3、0.1 .610.2 711 711.1 .711.2 712 .7 () .8 () 9 10III 317092012 1. Milk and milk powder. Determination of aflatoxin M1content. Clean-up by immunoaffinity chromatographyand determination by thin-layer chromatography 201307011 1 - . 1 2 , - 0,10 /3 .2 : IS 5725-12003 ( ) . 1. IS 5725-22003 ( ) . 2. - 17

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5、 M1, , . , 1()-. - . M1 -/. M1 . , M1 .5 - . 6709. - .5.1 5.1.1 20015.5.1.2 .5.1.3 22300.5.1.4 6995.5.1.5 5789.5.1.6 2603.5.1.7 .5.2 /, 3:2,303 (. 5.1.2) 20 3 (. 5.1.4), .5.3 /, 9:1,93 (. 5.1.5) 1 3 (. 5.1.2), -. - 1(. 5.5) .2 3170920125.4 5.4.1 100 3 4 3 (.5.1.4) 1 395 3 (.5.1.3) ( 95:4:1).5.4.2 10

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7、1 1 /3( ). - M1 0,50 /3.5.5.2.2 1003.9003/(.5.3), M1 0,1 /3( ). M1 0,10 /3.6 , :6.1 29227.6.2 .6.3 : - 25336.6.4 200 3 1770.6.5 100 3 1770.6.6 10 100 3.3 3170920126.7 53 - 1770.6.8 , , .6.9 , 200 400 , 10 .6.10 , 35 37 .6.11 ,2000g4.6.12 12026.6.13 . 1.- 100 1( 1 /310 /, 100 3 ). M1 80 % 4 ( 0,04 /3 0,40 / 100 3 ). , - .6.14 0,1 24104.6.15 .6.16 .6.17 .6.18 (), 50 .6.19 .6.20 , 10c18010 20c180 20 , - 60, .6.21 , 365 .6.22 .7 7.1 26809 2. , - .8 8.1 (. 6.10) (35 c1772) , , . (. 6.11) 2000 g 15 . -. - (. 6.12). 100 3 .8.2 20,0 0,1 250