1、 1. (ISO 14674:2005, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)317092012(ISO 14674:2005), - 1.092 . - 1.22009 . , . , , -, 1 - ( ) , - 42 3 , (- 1 2012 . 51) : ( 3166) 00497 ( 3166) 00497 BYKZKGMDRUTJ -4 29 2012.1772-317092012(ISO14674:2005) 1 2013 .5 ISO14674:
2、2005 Milk and milk powder. Determination of aflatoxin M1content. Clean-up by immunoaffinitychromatography and determination by thin-layer chromatography ( . 1. ). , , , - , -. - . (MOD). 5283120076 ( ) - . , - . - , 2013 -, II 3170920121 12 .13 24 .25 26 37 .48 48.1 .48.2 .48.3 59 .59.1 .59.2 510 61
3、0.1 .610.2 711 711.1 .711.2 712 .7 () .8 () 9 10III 317092012 1. Milk and milk powder. Determination of aflatoxin M1content. Clean-up by immunoaffinity chromatographyand determination by thin-layer chromatography 201307011 1 - . 1 2 , - 0,10 /3 .2 : IS 5725-12003 ( ) . 1. IS 5725-22003 ( ) . 2. - 17
4、7074 (IS 104283, IS 478880) . -, , , . 260379 . . 578978 . . 670972 . 699577 . -. 1202676 . 2001588 . 2230076 . . 241042001 . 2533682 . , - 2680986., 2922791 (IS 835-181) . . 1. - ( ) (1 317092012(ISO 14674:2005) ), 1 , - , . (), - () . , , , , - .3 -:3.1 1: 1, /3,1,/,- , .3.2 (recovery): , - , , .4
5、 M1, , . , 1()-. - . M1 -/. M1 . , M1 .5 - . 6709. - .5.1 5.1.1 20015.5.1.2 .5.1.3 22300.5.1.4 6995.5.1.5 5789.5.1.6 2603.5.1.7 .5.2 /, 3:2,303 (. 5.1.2) 20 3 (. 5.1.4), .5.3 /, 9:1,93 (. 5.1.5) 1 3 (. 5.1.2), -. - 1(. 5.5) .2 3170920125.4 5.4.1 100 3 4 3 (.5.1.4) 1 395 3 (.5.1.3) ( 95:4:1).5.4.2 10
6、03-10 3(.5.1.6) 33(.5.1.7) 873(.5.1.1) - ( 87:10:3).5.5 M15.5.1 M1 M1 M110 /3(. 5.1.1) 10 M113. 1 M1 200400 (. 5.1.1), . M1, 200400 . ()(c108),365.- , /3, c=c215100/c101, (1) , c108; M1,/( = 328 /);c101 M1 ,2/ (c101 = 1995 2/). M1 , , 0 . M1.5.5.2 M15.5.2.1 (.6.2) 50 3 -M1(.5.5.1). .- 5003/(.5.3), M
7、1 1 /3( ). - M1 0,50 /3.5.5.2.2 1003.9003/(.5.3), M1 0,1 /3( ). M1 0,10 /3.6 , :6.1 29227.6.2 .6.3 : - 25336.6.4 200 3 1770.6.5 100 3 1770.6.6 10 100 3.3 3170920126.7 53 - 1770.6.8 , , .6.9 , 200 400 , 10 .6.10 , 35 37 .6.11 ,2000g4.6.12 12026.6.13 . 1.- 100 1( 1 /310 /, 100 3 ). M1 80 % 4 ( 0,04 /3 0,40 / 100 3 ). , - .6.14 0,1 24104.6.15 .6.16 .6.17 .6.18 (), 50 .6.19 .6.20 , 10c18010 20c180 20 , - 60, .6.21 , 365 .6.22 .7 7.1 26809 2. , - .8 8.1 (. 6.10) (35 c1772) , , . (. 6.11) 2000 g 15 . -. - (. 6.12). 100 3 .8.2 20,0 0,1 250
