KS I ISO 10253-2008 Water quality－Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum《水质 用有中脉的骨条藻属和有三突的褐指藻属进行海洋藻类生长抑制试验》.pdf

1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 10253 KS I ISO 10253:2008 2008 11 10 http:/www.kats.go.krKS I ISO 10253:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：1998 3 26 ：2008 11 10 2008-0751 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 10253:2008 Water quality Marine a

2、lgal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum 2006 2 ISO 10253, Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum , . . . . . 1 Skeletonema costatum Phaeodactylum tricornutum . . KS M ISO 14442 KS M ISO 566

3、7 16 , , , , . 2 . . ( ) . KS M ISO 5667 16, 16： KS M ISO 14442, , , 3 , . 3.1 KS I ISO 10253:2008 2 (cell density) (x cells/mL) 3.2 (specific growth rate) )day/1(dd1txx= 3.3 (growth medium) ( ) 3.4 (test medium) , (3.3 ) 3.5 (test batch) , (3.4 ) . 3.6 (control) , (3.3 ) 3.7 xr )(EC x % 4 , , . (72

4、2) , (242) . ( ) . 5 5.1 . a) Skeletonema costatum(Greville) Cleve (CCAP 1077/1C, NIVA BAC 1) b) Phaeodactylum tricornutum Bohlin(CCAP 1052/1A, SAG 1090-1a, NIVA BAC 2) (phylum Bacillariophyta) KS I ISO 10253:2008 3 . . NIVA Norwegian Institute for Water Research P.O Box 173 Kjelss N-0411 Oslo Norwa

5、y CCAP Dunstaffnage Marine Laboratory P.O Box 3 Oban Argyll PA37 1QA United Kingdom SAG Collection of Algal Cultures University of Gttingen Albrecht-von-Haller Institute for Plant Science Untere Karsple 2 37073 Gttingen Germany 7.1 . Skeletonema , Phaeodactylum 2 3 . . 7.1 , 7.2 . (diatom) Phaeodact

6、ylum tricornutum .1 .1)5.2 , . . . 5.3 Phaeodactylum , (7.1) = (305) g/kg ( = 33 g/kg) . Skeletonema , . , 1) MICROBIOTESTS Inc., Venecoweg 19, 9810 Nazareth, Belgium, Tel (32) 9 380 8545, Fax (32) 9 380 8546, Email microbiotestsskynet.be . ISO . . KS I ISO 10253:2008 4 . 1 ( = 33 g/kg). . 1 g/L (Na

7、Cl) 22 6 (MgCl2 6H2O) 9.7 ( )(Na2SO4) 3.7 ( )(CaCl2) 1.0 (KCl) 0.65 (NaHCO3)0.20 H3BO3 0.023 0.45 m . 5.4 2 3 . 2 (1) FeCl3 6H2O 48 mg/L 149 g/L(Fe) MnCl2 4H2O 144 mg/L 605 g/L(Mn) ZnSO4 7H2O 45 mg/L 150 g/L(Zn) CuSO4 5H2O 0.157 mg/L 0.6 g/L(Cu) CoCl2 6H2O 0.404 mg/L 1.5 g/L(Co) H3BO3 1 140 mg/L 3.0

8、 mg/L(B) Na2EDTA 1 000 mg/L 15.0 mg/L (2) Thiamin hydrochloride 50 mg/L 25 g/L Biotin 0.01 mg/L 0.005 g/L Vitamin B12(cyanocobalamin) 0.10 mg/L 0.05 g/L (3) K3PO4 3.0 g/L 3.0 mg/L； 0.438 mg/L P NaNO3 50.0 g/L 50.0 mg/L； 8.24 mg/L N Na2SiO3 5H2O 14.9 g/L 14.9 mg/L； 1.97 mg/L Si (7.1 A ) . . 0.2 m . 1

9、 3 KS I ISO 10253:2008 5 120 15 . 4 , . 6 . . 6.1 7.6 . 6.2 . , (fluorimeter)(： in vitro 2) . (inoculum) . (10 cm ) , 104cells/mL . . 6.3 (250 mL) 6.4 0.2 m 0.45 m . 6.5 6.6 pH 7 7.1 900 mL (5.3) 15 mL (1), 0.5 mL (2) 1 mL (3)( 2 ) 1 L . pH 8.00.2 . EDTA KS I ISO 10253:2008 6 . KS M ISO 14442 . 7.2

10、2 4 (5.1 ). , 2103 cells/mL 104cells/mL 3 (7.1) . . . 7.3 3.2 ( 1.0 mg/L, 1.8 mg/L, 3.2 mg/L, 5.6 mg/L 10 mg/L) . EC(r )x . , 10 % 90 % . . . 7.4 (7.1) . KS M ISO 14442 KS M ISO 5667 16 , . ( , ) (5.4) , (5.3) . A . , pH . . pH ( ) pH 8.00.2 . . 7.5 (7.4), (7.1) (7.2) . , . 1.0 pH 64 ( ) (8. ). 104c

11、ells/mL . KS I ISO 10253:2008 7 Skeletonema (3 5 ) . Skeletonema . 3 . 6 ( ) . . . pH . 7.6 CO2 . 20 . 2 . 400700 nm 60 mol/m2 s 120 mol/m2 s . ( ) . ( .) “cosine” ( .) 1 . 1 0.35 m (30 W), KS C IEC 600813 2( 4 300 K) 47 . 2 lx 6 000 lx 10 000 lx . CO2 pH . 7.7 (242) ( ) . . (722) ( ) pH . 8 . a) (

12、) 72 16 . 0.9 d 1 (9.2) . KS I ISO 10253:2008 8 1 . 1.0 d 1 . b) ( ) 7 % . c) ( ) pH 1.0 . 2 pH 1.0 . . 9 9.1 . . . , . , . 9.2 (1) , . 0L0LInInttNN= (1) t0 ： tL ： ( )(9.1) N0 ： NL ： tL , (9.1) . ( ) . (2) . 100cc=iiI (2) Ii ： i ( ) KS I ISO 10253:2008 9 i ： i c ： ( ) 9.3 EC(r )x . , . EC(r )x ( , 4

13、, 5 6 ) . , ( ) . , EC(r )x . 10 EC(r )10 EC(r )50 EC10 EC50 . . , EC(r )50(0 72 ). EC(r )10 EC(r )50 (mg/L) (mL/L) . 11 EC10 EC50 . . EC10 EC50 , . ( ). 12 10 1989/1990 , (K2Cr2O7) 3,5 (Cl2C6H3OH) ISTPM/BAC/CCAP (1077/1C 1052/1B) 3 . 3 EC( r )50mg/L mg/L % Skeletonema costatum Potassium dichromate

14、9 2 2.5 (n=7) 1.1 44 3,5-dicholorophenol 7 2 1.6 (n=5) 0.3 18 Phaeodactylum tricornutum Potassium dichromate 10 3 20.1 (n=7) 5.3 26 3,5-dicholorophenol 10 3 2.7 (n=7) 0.2 8.6 . . KS I ISO 10253:2008 10 13 . a) (KS M ISO 10253： 2008) b) ： c) ： , , d) pH , e) ( ) ( ) ( ) , . EC(r )10 EC(r )50 , , KS I

15、 ISO 10253:2008 11 A ( ) ( ) A.1 ( ) (5.3 ) , 5.4 . , . A.2 135 mL (1), 4.5 mL (2) 9 mL (3) 700 mL (5.3) 1 L . 8.00.2 pH . A.3 A.1 (A.2), (7.2), ( ) ( 5.3) . . 7.5 . A.1 D (7.2) mL (7.4) mL (5.3) mL (A.2) mL mL 1/1.25 1 10 80 10 100 1/2 2 10 50 30 10 100 1/3 3 10 33.33 46.67 10 100 1/4 4 10 25 55 10

16、 100 1/5 5 10 20 60 10 100 1/8 8 10 12.5 67.5 10 100 1/12 12 10 8.33 71.67 10 100 1/16 16 10 6.25 73.75 10 100 1/24 24 10 4.17 75.83 10 100 1/32 32 10 3.125 76.875 10 100 10 80 10 100 KS I ISO 10253:2008 12 1 HERTZBERG, S. and JENSEN, A. Studies of alginate-immobilized marine microalgae. Botanica Ma

17、rina, 32, 1989, pp. 267273 2 MAYER, P., CUHEL, R.L. and NYHOLM, N. A simple in vitro fluorescence method for biomass measurements in algal growth inhibition tests. Water Research, 31, 1997, pp. 2 5252 531 3 KS C IEC 60081, 4 KOOIJMAN, S.A.L.M., HANSTVEIT, A.O. and OLDERSMA, H. Parametric analysis of

18、 population growth in bioassays. Water Research, 17, 1983, pp. 527538 5 NYHOLM, N., SRENSON, P.S., KUSK, K.O. and CHRISTENSEN, E.R. Statistical treatment of data from microbial toxicity tests, Environmental Tox. Chem., 11, 1992, pp. 157167 6 ISO/TS 202812), Water Quality Guidance on statistical interpretation of ecotoxicity data 2) 135513 7017 (02)60094114 (02)600948878 http:/