1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 10253 KS I ISO 10253:2008 2008 11 10 http:/www.kats.go.krKS I ISO 10253:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：1998 3 26 ：2008 11 10 2008-0751 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 10253:2008 Water quality Marine a
2、lgal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum 2006 2 ISO 10253, Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum , . . . . . 1 Skeletonema costatum Phaeodactylum tricornutum . . KS M ISO 14442 KS M ISO 566
3、7 16 , , , , . 2 . . ( ) . KS M ISO 5667 16, 16： KS M ISO 14442, , , 3 , . 3.1 KS I ISO 10253:2008 2 (cell density) (x cells/mL) 3.2 (specific growth rate) )day/1(dd1txx= 3.3 (growth medium) ( ) 3.4 (test medium) , (3.3 ) 3.5 (test batch) , (3.4 ) . 3.6 (control) , (3.3 ) 3.7 xr )(EC x % 4 , , . (72
4、2) , (242) . ( ) . 5 5.1 . a) Skeletonema costatum(Greville) Cleve (CCAP 1077/1C, NIVA BAC 1) b) Phaeodactylum tricornutum Bohlin(CCAP 1052/1A, SAG 1090-1a, NIVA BAC 2) (phylum Bacillariophyta) KS I ISO 10253:2008 3 . . NIVA Norwegian Institute for Water Research P.O Box 173 Kjelss N-0411 Oslo Norwa
5、y CCAP Dunstaffnage Marine Laboratory P.O Box 3 Oban Argyll PA37 1QA United Kingdom SAG Collection of Algal Cultures University of Gttingen Albrecht-von-Haller Institute for Plant Science Untere Karsple 2 37073 Gttingen Germany 7.1 . Skeletonema , Phaeodactylum 2 3 . . 7.1 , 7.2 . (diatom) Phaeodact
6、ylum tricornutum .1 .1)5.2 , . . . 5.3 Phaeodactylum , (7.1) = (305) g/kg ( = 33 g/kg) . Skeletonema , . , 1) MICROBIOTESTS Inc., Venecoweg 19, 9810 Nazareth, Belgium, Tel (32) 9 380 8545, Fax (32) 9 380 8546, Email microbiotestsskynet.be . ISO . . KS I ISO 10253:2008 4 . 1 ( = 33 g/kg). . 1 g/L (Na
7、Cl) 22 6 (MgCl2 6H2O) 9.7 ( )(Na2SO4) 3.7 ( )(CaCl2) 1.0 (KCl) 0.65 (NaHCO3)0.20 H3BO3 0.023 0.45 m . 5.4 2 3 . 2 (1) FeCl3 6H2O 48 mg/L 149 g/L(Fe) MnCl2 4H2O 144 mg/L 605 g/L(Mn) ZnSO4 7H2O 45 mg/L 150 g/L(Zn) CuSO4 5H2O 0.157 mg/L 0.6 g/L(Cu) CoCl2 6H2O 0.404 mg/L 1.5 g/L(Co) H3BO3 1 140 mg/L 3.0
8、 mg/L(B) Na2EDTA 1 000 mg/L 15.0 mg/L (2) Thiamin hydrochloride 50 mg/L 25 g/L Biotin 0.01 mg/L 0.005 g/L Vitamin B12(cyanocobalamin) 0.10 mg/L 0.05 g/L (3) K3PO4 3.0 g/L 3.0 mg/L； 0.438 mg/L P NaNO3 50.0 g/L 50.0 mg/L； 8.24 mg/L N Na2SiO3 5H2O 14.9 g/L 14.9 mg/L； 1.97 mg/L Si (7.1 A ) . . 0.2 m . 1
9、 3 KS I ISO 10253:2008 5 120 15 . 4 , . 6 . . 6.1 7.6 . 6.2 . , (fluorimeter)(： in vitro 2) . (inoculum) . (10 cm ) , 104cells/mL . . 6.3 (250 mL) 6.4 0.2 m 0.45 m . 6.5 6.6 pH 7 7.1 900 mL (5.3) 15 mL (1), 0.5 mL (2) 1 mL (3)( 2 ) 1 L . pH 8.00.2 . EDTA KS I ISO 10253:2008 6 . KS M ISO 14442 . 7.2
10、2 4 (5.1 ). , 2103 cells/mL 104cells/mL 3 (7.1) . . . 7.3 3.2 ( 1.0 mg/L, 1.8 mg/L, 3.2 mg/L, 5.6 mg/L 10 mg/L) . EC(r )x . , 10 % 90 % . . . 7.4 (7.1) . KS M ISO 14442 KS M ISO 5667 16 , . ( , ) (5.4) , (5.3) . A . , pH . . pH ( ) pH 8.00.2 . . 7.5 (7.4), (7.1) (7.2) . , . 1.0 pH 64 ( ) (8. ). 104c
11、ells/mL . KS I ISO 10253:2008 7 Skeletonema (3 5 ) . Skeletonema . 3 . 6 ( ) . . . pH . 7.6 CO2 . 20 . 2 . 400700 nm 60 mol/m2 s 120 mol/m2 s . ( ) . ( .) “cosine” ( .) 1 . 1 0.35 m (30 W), KS C IEC 600813 2( 4 300 K) 47 . 2 lx 6 000 lx 10 000 lx . CO2 pH . 7.7 (242) ( ) . . (722) ( ) pH . 8 . a) (
12、) 72 16 . 0.9 d 1 (9.2) . KS I ISO 10253:2008 8 1 . 1.0 d 1 . b) ( ) 7 % . c) ( ) pH 1.0 . 2 pH 1.0 . . 9 9.1 . . . , . , . 9.2 (1) , . 0L0LInInttNN= (1) t0 ： tL ： ( )(9.1) N0 ： NL ： tL , (9.1) . ( ) . (2) . 100cc=iiI (2) Ii ： i ( ) KS I ISO 10253:2008 9 i ： i c ： ( ) 9.3 EC(r )x . , . EC(r )x ( , 4
13、, 5 6 ) . , ( ) . , EC(r )x . 10 EC(r )10 EC(r )50 EC10 EC50 . . , EC(r )50(0 72 ). EC(r )10 EC(r )50 (mg/L) (mL/L) . 11 EC10 EC50 . . EC10 EC50 , . ( ). 12 10 1989/1990 , (K2Cr2O7) 3,5 (Cl2C6H3OH) ISTPM/BAC/CCAP (1077/1C 1052/1B) 3 . 3 EC( r )50mg/L mg/L % Skeletonema costatum Potassium dichromate
14、9 2 2.5 (n=7) 1.1 44 3,5-dicholorophenol 7 2 1.6 (n=5) 0.3 18 Phaeodactylum tricornutum Potassium dichromate 10 3 20.1 (n=7) 5.3 26 3,5-dicholorophenol 10 3 2.7 (n=7) 0.2 8.6 . . KS I ISO 10253:2008 10 13 . a) (KS M ISO 10253： 2008) b) ： c) ： , , d) pH , e) ( ) ( ) ( ) , . EC(r )10 EC(r )50 , , KS I
15、 ISO 10253:2008 11 A ( ) ( ) A.1 ( ) (5.3 ) , 5.4 . , . A.2 135 mL (1), 4.5 mL (2) 9 mL (3) 700 mL (5.3) 1 L . 8.00.2 pH . A.3 A.1 (A.2), (7.2), ( ) ( 5.3) . . 7.5 . A.1 D (7.2) mL (7.4) mL (5.3) mL (A.2) mL mL 1/1.25 1 10 80 10 100 1/2 2 10 50 30 10 100 1/3 3 10 33.33 46.67 10 100 1/4 4 10 25 55 10
16、 100 1/5 5 10 20 60 10 100 1/8 8 10 12.5 67.5 10 100 1/12 12 10 8.33 71.67 10 100 1/16 16 10 6.25 73.75 10 100 1/24 24 10 4.17 75.83 10 100 1/32 32 10 3.125 76.875 10 100 10 80 10 100 KS I ISO 10253:2008 12 1 HERTZBERG, S. and JENSEN, A. Studies of alginate-immobilized marine microalgae. Botanica Ma
17、rina, 32, 1989, pp. 267273 2 MAYER, P., CUHEL, R.L. and NYHOLM, N. A simple in vitro fluorescence method for biomass measurements in algal growth inhibition tests. Water Research, 31, 1997, pp. 2 5252 531 3 KS C IEC 60081, 4 KOOIJMAN, S.A.L.M., HANSTVEIT, A.O. and OLDERSMA, H. Parametric analysis of
18、 population growth in bioassays. Water Research, 17, 1983, pp. 527538 5 NYHOLM, N., SRENSON, P.S., KUSK, K.O. and CHRISTENSEN, E.R. Statistical treatment of data from microbial toxicity tests, Environmental Tox. Chem., 11, 1992, pp. 157167 6 ISO/TS 202812), Water Quality Guidance on statistical interpretation of ecotoxicity data 2) 135513 7017 (02)60094114 (02)600948878 http:/
