KS I ISO 11268-3-2005 Soil quality－Effects of pollutants on earthworms－Part 3：Guidance on the determination of effects in field situations《土壤质量 污染物对蚯蚓的影响 第3部分 野外影响的测定指南》.pdf

1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2005 12 28 KS I ISO 112683 3： KS I ISO 112683：2005I ISO 112683：2005 ( ) ( ) KIST ( ) ( ) ： ： 2001 1 6 ： 2005 12 28 2005 1014 ： ： ( ) ( 02 509 72625) . 7 5 , . ICS 13.080.30 KS I ISO 3： 11268 3： 2005Soil quality Effects of pollutants on earthworms Part 3： Gu

2、idance on the determination of effects in field situations 1999 1 ISO 11268 3 Soil quality Effects of pollutants on earthworms Part 3： Guidance on the determination of effects in field situations . 1. , (chemicals) . 2. . . KS M ISO 10390 pH KS M ISO 10694 ( ) KS M ISO 11274 ( ) KS M ISO 11277 ( , )

3、 3. (kg/ha) (L/ha) . . 4. . 1 . . , . 4 . . , (residue analysis) . 5. 5.1 (screen flotation) . I ISO 112683：2005 2 . (Raw 1959) (Oktett method, Thielemann 1986, Cuendet et al. 1991) . . (Lee 1985). (individual samples) . 5.2 510 L/0.25 m2 0.2 % . 23 . 30 . (5 % formol 70 % , 8.1 ) . (30 ) ( , Aporre

4、ctodea rosea ) . 5.3 1 60 g . 9 L . 5.2 . 5.4 . , , , . . 30 . (30 ) . 6. 6.1 (test site) 6.1.1 (記述) . , . . 1 m2 20 . 1 m2 100 . 6.1.3 . (type of environment) . , Lumbricus terrestris Aporrectodea caliginosa ( 10 % ) . . . , , . , . (KS M ISO 11277) (KS M ISO 10694) I ISO 112683：2005 3 pH (KS M ISO

5、 10390) (A ) . (micro-climate) ( , , , ) , . ( , , ). 3 . 6.1.2 (test field) (1 26 ) . 12 . . , . , . . ( ) . . ( ) . . . . . 12 . 6.1.3 (design of experiment) . . ( ) 100 m2(10 m10 m) . . 1 m 2 m ( 1 ). I ISO 112683：2005 4 2 1 1 2 (edge strip) 1 2 m . . (Daniel & Bieri 1988). ( , , ) 4 , 4 ( , 16 .

6、). 0.25 m2 . 1015 cm, 56 cm . 1 m2 . . . 6.2 . 3050 cm( .) . . (diapause) . 60 % . 3 . , , . 7. 7.1 (application of the test substance) . , . ( ) , , . , I ISO 112683：2005 5 (application equipment) ( , .). . 200300 L/ha . 400800 L/ha . . ( ) , . . . . . 7.2 3 , . ： 1 ： 46 ： 12 . . 7.3 ( ) . (Niklas

7、& Kennel 1978, Edwards & Brown 1982, Heimbach 1990). (4080 % ) 24 kg/ha . . 8. 8.1 (Graff 1953, Sims&Gerard, 1985) . Easton(1983) . 5 % . 70 % (fixation) (preservation liquid) . , . , . . Tanylobes Epilobes . Epilobous Tanylobous . peristomium (prostomium) . . Lumbricus spp I ISO 112683：2005 6 ( ) .

8、 Zygolobous peristomium ( 1 ) , Prolobous posterior Epilobous , Epilobous Tanylobous . 2() . 8.2 . . 9. . . (test) (inference) . Kolmogoroff Smirnovs(1) Bartletts . Dunnetts Williams ( = 0.05, one-sided) t (multiple t-test) . U (multiple U-test), Holm(1979) Bonferroni U . (parametric test procedures

9、) ( , ) pairwise Students t- Mann Whitney U . (1) Kolmogoroff Smirnovs 2 ( ) . (activity-related) , . (Cuendet et al. 1991) Lumbricus terrestris . (endogeneous species) ( ) (Raw 1959). 10. . a) b) 9. c) , d) (6.1.1 ) e) f) ( , , ) g) h) i) , j) k) l) m) n) I ISO 112683：2005 7 1() I ISO 112683：2005 8

10、 2() KolmogoroffSmirnov KolmogoroffSmirnov (degree of agreement) , x2 . . ： . 1. . “H0： .” . D . D = maximum )()(N0XSXF F0(X)： , x SN(X)： , SN(X ) =K/N K： x N： KolmogoroffSmirnov , . a) . b) . c) . 1. . “H0= .” “ .” . . D D = maximum )()(21XSXSnn Sn1(X)： , Sn1(X ) =K/n1Sn2(X)： , Sn2(X ) =K/n2K： x n1

11、,n2： n1,n2 40 D K kd kd . 40 n1 n2 , kd . I ISO 112683：2005 9 2. 40 20 . 40 n1 n2 . D x2 . x2 x2 = 4D22121nnnn+ . ventrral(low surface) view (dorsal cross -section upper surface) I ISO 112683：2005 10 (L, terrestris) (dorsal cross -section upper surface) 3： 2006 1 23 135 513 701 7 (02)6009 4567 (02)6

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14、 7 Fax. (062)953 1438 641 210 298 7 ( 6 ) (055)212 1212 Fax. (055)212 1213 683 804 758 2( 2 ) (052)289 6601 3 Fax. (052)289 6604 425 020 541 3( 4 ) (031)487 6191 4 Fax. (031)487 6195 730 350 92 30( 8 ) (054)473 6954 Fax. (054)473 6955 480 848 2 487 6( 3 ) (031)82981824 Fax. (031)8298185 KOREAN STANDARDS ASSOCIATION KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKS I ISO 112683： 2005 Soil qualityEffects of pollutants on earthwormsPart 3：Guidance on the determination of effects in field situations ICS 13.080.30