1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS J ISO 4833 30 KS J ISO 4833 :2007 (2012 ) 2007 10 31 http:/www.kats.go.krKS J ISO 4833:2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) : :2002 10 31 :2007 10 31 :2012 12 28 : 20120814 : ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 48
2、33:2007 (2012 ) 30 Microbiology of food and animal feeding stuffsHorizontal method for the enumeration of microorganismsColony-count technique at 30 2003 3 ISO 4833, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganismsColony-count technique at 30 , .
3、 ISO 4833 ISO/TC 34 (SC 9) . 3 ISO 4833 ISO 4833:1991 2 . 1 30 . . . 2 . . ( ) . KS H ISO 6887( ), , 10 KS H ISO 7218, ISO 8261, Milk and milk productsGeneral guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination ISO/TS 111331, Microb
4、iology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 1:General guidelines on quality assurance for the preparation of culture media in the laboratory 3 . KS J ISO 4833:2007 3.1 , 4 2 . 10 . 4.1 30 72 . 4.2 g mL (10. ). 5 KS H ISO 7218 ISO/TS 111331 .
5、5.1 KS H ISO 6887 . 5.2 (plate count agar) 5.2.1 (enzymatic digestion of casein) 5.0 g (yeast extract) 2.5 g (anhydrous glucose) 1.0 g (agar) a9 18 g 1 000 mL a . mL 1.0 g (skimmed milk) . (skimmed milk) . 5.2.2 5.2.2.1 (skimmed milk) . pH 25 7.00.2 . 5.2.2.2 2 KS J ISO 4833:2007 , , (skimmed milk)
6、. . . pH 25 7.00.2 5.2.2.3 , 1215 mL (6.8 ) , 500 mL (6.8 ) . 121 15 . 4447 (6.5 ) . (32) 3 . , 4447 (6.5 ) . 15 g/L . . 5.2.3 ISO/TS 111331 . 5.3 (overlay medium) ( 9.2.7 ) 5.3.1 (agar) a1218 g 1 000 mL a . 5.3.2 30 . pH 25 7.00.2 . 5.3.3 , 4 mL(6.8 ) , (6.8 ) . 121 15 . 3 KS J ISO 4833:2007 , 4447
7、 (6.5 ) . (32) 3 . 4447 (6.5 ) . 6 . . 6.1 () ( ) KS H ISO 7218 . 6.2 (301) . 6.3 90100 mm . 6.4 1 mL 6.5 4447 . 6.6 . 6.7 pH 25 pH 0.1 . 6.8 , , 500 mL . 7 . . . . 8 4 KS J ISO 4833:2007 KS H ISO 6887 ISO 8261 . . 9 9.1 , KS H ISO 6887 . 9.2 9.2.1 2 (6.3 ) . (6.4 ) 1 mL, 1 mL (10 1). 9.2.2 2 (6.3 )
8、 . (6.4 ) 10 (10 1 ) 1 mL, 10 (10 2 ) 1 mL . 9.2.3 10 . 9.2.4 ( 2 10 ) 15300 . 9.2.5 4447 (5.2 ) 1215 mL . ( 10 1) (5.2 ) 45 . 9.2.6 . 9.2.7 4447 (overlay medium)(5.3 ) 4 mL . . 9.2.8 (723) (301) (6.2 ) . 6 . . 9.3 9.3.1 (9.2.8 ) (10.1 ) (6.6 ) . . 10 10.1 5 KS J ISO 4833:2007 ISO 7218:2002 Amendmen
9、t 1 . 10.2 10.2.1 15300 . . ( 1, 2 3 ) , (pasteurized milk) . . 10.2.2 , mL log 10 r0.25( mL 1.8 ) . ( 1, 2 3 ) . 10.2.3 , mL log 10 R 0.45( mL 2.8 ) . ( 1, 2 3 ) . 10.3 , 95 % , . . mL . a) :10 5 100 000 0.25 log 10 . :log10 4.75 56 000 log10 5.25 178 000 56 000 178 000 . b) ( ):10 5 100 000 6 KS J
10、 ISO 4833:2007 0.45 log 10 . :log10 4.55 36 000 log10 5.45 280 000 36 000 280 000 . A critical difference(CD) . 10.4 KS H ISO 7218 . 11 . 7 KS J ISO 4833:2007 A () critical difference(CD) , 95 % , . . mL . a) ( ):10 5 100 000 ( n : n2) log 10 critical difference(CD) . 41 . 0 2 25 . 0 45 . 0 2 1 1 2
11、2 2 2 r R n r R CD r : R : 10 4.59 39 000 10 5.41 257 000 . b) (one-sided test) :10 5 100 000 ( n : n2) critical difference limit(CDL) . 24 . 0 2 2 84 . 0 1 1 2 84 . 0 2 2 2 2 r R n r R CDL 10 5.24 174 000 . 8 KS J ISO 4833:2007 9 1 PITON, C., GRAPPIN, R. A model for statistical evaluation of precis
12、ion parameters of microbiological methods:Application to dry rehydratable film methods and IDG reference methods for enumeration of total aerobic mesophilic flora and coliforms in raw milk. J. AOAC, 74, 1991, pp. 92103 2 SCOTTER, S., ALDRIGE, M., BACK, J., Wood, R. Validation of European Community m
13、ethods for microbiological and chemical analysis of raw and heat-treated milk. J. Assoc. Publ. Analyst., 29, 1993, pp.132 3 DAHMS. S., WEISS, H. Estimation of precision values for microbiological reference methods: Standardized pour plate technique. Milchwiss., 53, 1988, pp 555559 30 153787 1 145 3(
14、16) (02)26240114 (02)26240148 http:/ KS J ISO 4833 :2007 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganisms Colony-count technique at 30 ICS 07.100.30 Korean Agency for Technology and Standards http:/www.kats.go.kr
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