1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS J ISO 4833 30 KS J ISO 4833 ：2007 (2012 ) 2007 10 31 http:/www.kats.go.krKS J ISO 4833：2007 : ( ) ( ) () () () ( ) : (http:/www.standard.go.kr) ： ：2002 10 31 ：2007 10 31 ：2012 12 28 ： 20120814 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS J ISO 48
2、33：2007 (2012 ) 30 Microbiology of food and animal feeding stuffsHorizontal method for the enumeration of microorganismsColony-count technique at 30 2003 3 ISO 4833, Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganismsColony-count technique at 30 , .
3、 ISO 4833 ISO/TC 34 (SC 9) . 3 ISO 4833 ISO 4833：1991 2 . 1 30 . . . 2 . . ( ) . KS H ISO 6887( ), , 10 KS H ISO 7218, ISO 8261, Milk and milk productsGeneral guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination ISO/TS 111331, Microb
4、iology of food and animal feeding stuffsGuidelines on preparation and production of culture mediaPart 1：General guidelines on quality assurance for the preparation of culture media in the laboratory 3 . KS J ISO 4833：2007 3.1 , 4 2 . 10 . 4.1 30 72 . 4.2 g mL (10. ). 5 KS H ISO 7218 ISO/TS 111331 .
5、5.1 KS H ISO 6887 . 5.2 (plate count agar) 5.2.1 (enzymatic digestion of casein) 5.0 g (yeast extract) 2.5 g (anhydrous glucose) 1.0 g (agar) a9 18 g 1 000 mL a . mL 1.0 g (skimmed milk) . (skimmed milk) . 5.2.2 5.2.2.1 (skimmed milk) . pH 25 7.00.2 . 5.2.2.2 2 KS J ISO 4833：2007 , , (skimmed milk)
6、. . . pH 25 7.00.2 5.2.2.3 , 1215 mL (6.8 ) , 500 mL (6.8 ) . 121 15 . 4447 (6.5 ) . (32) 3 . , 4447 (6.5 ) . 15 g/L . . 5.2.3 ISO/TS 111331 . 5.3 (overlay medium) ( 9.2.7 ) 5.3.1 (agar) a1218 g 1 000 mL a . 5.3.2 30 . pH 25 7.00.2 . 5.3.3 , 4 mL(6.8 ) , (6.8 ) . 121 15 . 3 KS J ISO 4833：2007 , 4447
7、 (6.5 ) . (32) 3 . 4447 (6.5 ) . 6 . . 6.1 () ( ) KS H ISO 7218 . 6.2 (301) . 6.3 90100 mm . 6.4 1 mL 6.5 4447 . 6.6 . 6.7 pH 25 pH 0.1 . 6.8 , , 500 mL . 7 . . . . 8 4 KS J ISO 4833：2007 KS H ISO 6887 ISO 8261 . . 9 9.1 , KS H ISO 6887 . 9.2 9.2.1 2 (6.3 ) . (6.4 ) 1 mL, 1 mL (10 1). 9.2.2 2 (6.3 )
8、 . (6.4 ) 10 (10 1 ) 1 mL, 10 (10 2 ) 1 mL . 9.2.3 10 . 9.2.4 ( 2 10 ) 15300 . 9.2.5 4447 (5.2 ) 1215 mL . ( 10 1) (5.2 ) 45 . 9.2.6 . 9.2.7 4447 (overlay medium)(5.3 ) 4 mL . . 9.2.8 (723) (301) (6.2 ) . 6 . . 9.3 9.3.1 (9.2.8 ) (10.1 ) (6.6 ) . . 10 10.1 5 KS J ISO 4833：2007 ISO 7218：2002 Amendmen
9、t 1 . 10.2 10.2.1 15300 . . ( 1, 2 3 ) , (pasteurized milk) . . 10.2.2 , mL log 10 r0.25( mL 1.8 ) . ( 1, 2 3 ) . 10.2.3 , mL log 10 R 0.45( mL 2.8 ) . ( 1, 2 3 ) . 10.3 , 95 % , . . mL . a) ：10 5 100 000 0.25 log 10 . ：log10 4.75 56 000 log10 5.25 178 000 56 000 178 000 . b) ( )：10 5 100 000 6 KS J
10、 ISO 4833：2007 0.45 log 10 . ：log10 4.55 36 000 log10 5.45 280 000 36 000 280 000 . A critical difference(CD) . 10.4 KS H ISO 7218 . 11 . 7 KS J ISO 4833：2007 A () critical difference(CD) , 95 % , . . mL . a) ( )：10 5 100 000 ( n ： n2) log 10 critical difference(CD) . 41 . 0 2 25 . 0 45 . 0 2 1 1 2
11、2 2 2 r R n r R CD r ： R ： 10 4.59 39 000 10 5.41 257 000 . b) (one-sided test) ：10 5 100 000 ( n ： n2) critical difference limit(CDL) . 24 . 0 2 2 84 . 0 1 1 2 84 . 0 2 2 2 2 r R n r R CDL 10 5.24 174 000 . 8 KS J ISO 4833：2007 9 1 PITON, C., GRAPPIN, R. A model for statistical evaluation of precis
12、ion parameters of microbiological methods：Application to dry rehydratable film methods and IDG reference methods for enumeration of total aerobic mesophilic flora and coliforms in raw milk. J. AOAC, 74, 1991, pp. 92103 2 SCOTTER, S., ALDRIGE, M., BACK, J., Wood, R. Validation of European Community m
13、ethods for microbiological and chemical analysis of raw and heat-treated milk. J. Assoc. Publ. Analyst., 29, 1993, pp.132 3 DAHMS. S., WEISS, H. Estimation of precision values for microbiological reference methods： Standardized pour plate technique. Milchwiss., 53, 1988, pp 555559 30 153787 1 145 3(
14、16) (02)26240114 (02)26240148 http:/ KS J ISO 4833 ：2007 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of microorganisms Colony-count technique at 30 ICS 07.100.30 Korean Agency for Technology and Standards http:/www.kats.go.kr
